[1]赵晋铭,孟庆长,张玉梅,等.菜用大豆百粒鲜重QTL定位[J].大豆科学,2007,26(06):853-856,878.[doi:10.3969/j.issn.1000-9841.2007.06.009]
　ZHAO Jin-ming,MENG Qing-chang,ZHANG Yu-mei,et al.QTL MAPPING FOR 100-SEED FRESH WEIGHT IN VEGETABLE SOYBEAN[J].Soybean Science,2007,26(06):853-856,878.[doi:10.3969/j.issn.1000-9841.2007.06.009]
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菜用大豆百粒鲜重QTL定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 853-856,878 栏目: 出版日期: 2007-12-25
Title:
QTL MAPPING FOR 100-SEED FRESH WEIGHT IN VEGETABLE SOYBEAN
文章编号:
1000-9841(2007)06-0853-04
作者:
赵晋铭; 孟庆长; 张玉梅; 张祎囡; 盖钧镒; 喻德跃; 邢邯
国家大豆改良中心，南京农业大学农学院，作物遗传与种质创新国家重点实验室，南京 210095
Author(s):
ZHAO Jin-ming; MENG Qing-chang; ZHANG Yu-mei; ZHANG Yi-nan; GAI Jun-yi; YU De-yue; XING Han
National Center of Soybean Improvement/College of Agriculture，Nanjing Agricultral University/National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nan jing 210095
关键词:
菜用大豆; 百粒鲜重; QTL定位
Keywords:
Vegetable soybean; 100-seed fresh weight; QTL Mapping
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.009
文献标志码:
A
摘要:
百粒鲜重是衡量菜用大豆品质的重要指标之一，以NJ(SP)BN（BOGAO×NG94-156）的RIL群体158个家系为研究材料，于2005年和2006年测定该RIL群体的百粒鲜重并构建遗传图谱进行QTL定位。采用Win QTL Cart V2.5中的复合区间作图法进行QTL分析，结果表明，两年资料对百粒鲜重QTL的定位结果基本一致，其中位于G连锁群上的一个QTL在两年中均被检测到，可解释7.64％～12.74％的表型变异。此外利用QTL Mapper 1.6对以上定位结果进行验证并估计与环境的互作效应，结果表明，定位到一个加性QTL（qss-5）与环境存在显著的互作效应，效应值为1.18％。本研究筛选到的与百粒鲜重紧密连锁的SSR标记，为菜用大豆分子标记辅助选择育种提供了理论基础。
Abstract:
100-seed fresh weight is an important quality trait of vegetable soybean. The objective of the present study was to map QTL for 100-seed fresh weight of vegetable soybean. The Recombinant Inbred Lines(RIL)，including 158 lines，from the cross BOGAO×NG94-156，were used as experimental materials in this study. Based on the linkage map constructed mainly with Single Sequence Repeat (SSR）markers using this RIL population，the software WinQTL Cart V2-5 and the composite interval mapping were employed to identify quantitative traits loci(QTL）associated with 100-seed fresh weight in 2005 and 2006 years. It was found that the results of mapping QTL for 100-seed fresh weight were similar for these two years. Two QTLs mapped on linkage group G could be detected in two years，and they could be explained 7.64％～12.74％ of the total variation of the 100-seed fresh weight，respectively. In addition，the software QTL Mapper 1.6 was applied to detect QTLs and estimate additive environment interaction effects of QTLs. The result indicated that the results of mapping QTL for 100-seed fresh weight were similar for these two methods. There was one QTL with additive -environment interaction effect，and the value is 1.18%.The SSR marker acquired by QTL mapping for 100-seed fresh weight in this study，was served as a solid platform for the molecular marker assisted selection.

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备注/Memo

基金项目：江苏省科学技术厅高技术项目（G2006308）； 教育部长江学者和创新团队发展计划项目（IRT0432）

作者简介：赵晋铭(1978－)，男，博士研究生, 主要从事分子数量遗传与作物遗传育种研究。 E-mail: jmz3000@126.com
通讯作者：邢邯, 教授。E-mail: hanx@njau.edu.cn

更新日期/Last Update: 2014-10-19