[1]吴迪,刘斌,侯文胜,等.转座子标签法及其在大豆功能基因组研究中的应用前景[J].大豆科学,2007,26(02):254-258.[doi:10.3969/j.issn.1000-9841.2007.02.028]
　WU Di,LIU Bin,HOU Wen-sheng,et al.TRANSPOSON TAGGING AND ITS POTENTIAL USE IN FUNCTIONAL GENOMICS STUDY OF SOYBEAN[J].Soybean Science,2007,26(02):254-258.[doi:10.3969/j.issn.1000-9841.2007.02.028]
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转座子标签法及其在大豆功能基因组研究中的应用前景

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年02期 页码: 254-258 栏目: 出版日期: 2007-04-25
Title:
TRANSPOSON TAGGING AND ITS POTENTIAL USE IN FUNCTIONAL GENOMICS STUDY OF SOYBEAN
文章编号:
1000-9841(2007)02-025405
作者:
吴迪1; 2; 刘斌1; 3; 侯文胜1; 3; 朱延明2; 韩天富1
1.中国农业科学院作物科学研究所，北京 100081；
2.东北农业大学生命科学学院，哈尔滨 150030；
3.西北农林科技大学,杨凌 712100
Author(s):
WU Di1; 2; LIU Bin1; 3; HOU Wen-sheng1; 3; ZHU Yan-ming2; HAN Tian-fu1
1.Institute of Crop Science,The Chinese Academy of Agricultural Sciences,Beijing 100081;
2.College of Life Science,Northeast Agricultural University,Harbin 150030;?
3 Northwest Agricultural and Forestry Sci-tech University,Yangling 712100
关键词:
转座子标签法; 大豆; 基因克隆; 双因子标签法
Keywords:
Transposon tagging; Soybean; Gene cloning; Two element transposon tagging system
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.02.028
文献标志码:
A
摘要:
转座子标签法是克隆未知基因的有效方法之一。本文介绍了转座子标签法的基本原理、步骤、方法及其在植物基因克隆中的应用情况，并对该方法在大豆突变体库构建和功能基因组研究中的应用前景进行了讨论。
Abstract:
Transposon tagging is a powerful molecular tool for isolating genes with unknown functions. This paper reviewed the fundamental,procedure and method of transposon tagging,as well as the recent advances of transposon uses in gene cloning. The application of transposon tagging in the mutant pool establishment and functional genomics study of soybean was also discussed and prospected.

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备注/Memo

基金项目：国家重点基础研究发展计划（973）项目（2002CB111301-3）；北京市自然科学基金（5042019）和农业部作物遗传育种重点开放实验室资助。

作者简介：吴迪（1979－），女，博士，从事大豆分子生物学研究。刘斌与第一作者有同等贡献。

更新日期/Last Update: 2014-10-22