[1]穆筱梅,钟振声,杨梅.脂质体用大豆卵磷脂的制备及质量评价[J].大豆科学,2007,26(02):250-253.[doi:10.3969/j.issn.1000-9841.2007.02.027]
　MU Xiao-mei,ZHONG Zhen-sheng,YANG Mei.PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL[J].Soybean Science,2007,26(02):250-253.[doi:10.3969/j.issn.1000-9841.2007.02.027]
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脂质体用大豆卵磷脂的制备及质量评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年02期 页码: 250-253 栏目: 出版日期: 2007-04-25
Title:
PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL
文章编号:
1000-9841(2007)02-0250-04
作者:
穆筱梅1; 钟振声2; 杨梅2
1.仲恺农业技术学院化学与化工系，广东 510325；
2.华南理工大学化学学院，广州 510641
Author(s):
MU Xiao-mei1; ZHONG Zhen-sheng2; YANG Mei1
1.Department of Chemistry & Chemical Engineering,Zhongkai University of Agriculture and Technology,Guangzhou 510225；
2.College of Chemistry,South China University of Technology,Guangzhou,510641
关键词:
大豆卵磷脂; 溶剂萃取; 超临界CO2萃取; 脂质体
Keywords:
Soybean phosphatidylcholines; Solvent extraction; Supercritical carbon dioxide extraction; and liposome
分类号:
TS224.4
DOI:
10.3969/j.issn.1000-9841.2007.02.027
文献标志码:
A
摘要:
以大豆磷脂为原料，用溶剂－超临界CO2萃取法制备脂质体用高纯度大豆卵磷脂，并对其质量进行评价。结果表明大豆卵磷脂的含量是94.98%，丙酮不溶物的含量是99.0%，酸值是20.6 KOH mg/g，干燥失重是0.63%，己烷不溶物的含量0.18%，砷盐的含量是2.1×10-6%，重金属的含量是2.0×10-5%。以精制的大豆磷脂为原料制备的脂质体，在透射电镜下呈圆球状。粒径为164 nm，Zeta电位为-28.50 mv，包封率是32.43%。
Abstract:
High-purity soybean phosphatidylcholines(SPC) as liposomal material was prepared from soybean phospholipids with a method combined super critical carbon dioxide extraction and solvent extraction. The results of the quality evaluation showed that the SPC was separated with94.98% purity,the content of aceton-insoluble matter,hexane-insoluble matter,heavy metals and arsenic were respectively 99%,0.18%,2.0 10-5% and 2.1 10-6%,the acid value was 20.6 mg of potassium hydroxide,loss on drying of SPC was 0.63%.The liposome made with SPC showed spherical shape,its main particles was164 nm ,Zeta potential was -28.50 mv,and the entrapment efficiency was 32.43%.

参考文献/References:

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［3］张灵芝.脂质体制备及其在生物医学中的应用[M].北京:北京医科大学、北京协和医科大学联合出版社,1998：1-53.
［4］董晓渭,冯学伟,李桂贞.高效液相法测定大豆磷脂中的磷脂酰胆碱[J].华东理工大学学报,2000,(26):315-317.
［5］Soka F C,Papahadjopoulos D. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse evaporation[J].Process of National Science,1978,75:4194-4198.
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相似文献/References:

[1]贾乃堃 袁其朋.高纯度大豆黄苷及大豆黄素的制备[J].大豆科学,2004,23(01):11.[doi:10.11861/j.issn.1000-9841.2004.01.0011]
　Jia Naikun Yuan Qipeng.PREPARATION OF THE HIGH PURITY DAIDZIN AND SAIDZEIN[J].Soybean Science,2004,23(02):11.[doi:10.11861/j.issn.1000-9841.2004.01.0011]

备注/Memo

作者简介：穆筱梅（1973—），女，讲师，主要从事大豆磷脂的精制以及脂质体的研究。

更新日期/Last Update: 2014-10-22