[1]胡蕴珠　智海剑 沈文飚　徐朗莱.对SMV 不同抗性的大豆品种体内过氧化物酶活性变化的研究[J].大豆科学,1997,16(01):28-33.[doi:10.11861/j.issn.1000-9841.1997.01.0028]
　Hu YunzhuZhi Hai jia nShen WenbiaoXu La ng lai.STUDIES ON THE CHANGES OF PEROXIDASE ACTIVITY INDIFFERENT RESISTANT SOYBEAN CULTIVARS TO SMV[J].Soybean Science,1997,16(01):28-33.[doi:10.11861/j.issn.1000-9841.1997.01.0028]
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对SMV 不同抗性的大豆品种体内过氧化物酶活性变化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第16卷 期数: 1997年01 页码: 28-33 栏目: 出版日期: 1997-02-20
Title:
STUDIES ON THE CHANGES OF PEROXIDASE ACTIVITY INDIFFERENT RESISTANT SOYBEAN CULTIVARS TO SMV
作者:
胡蕴珠　智海剑 沈文飚　徐朗莱
(南京农业大学大豆研究所,南京210095)
(南京农业大学生化教研组210095)
Author(s):
Hu Yunzhu　Zhi Hai jia n　Shen Wenbiao　Xu La ng lai
( Nanjing Agricultural University , N anj ing , 210095)
关键词:
大豆;  大豆花叶病毒; 过氧化物酶
Keywords:
Soy bean mo saic vi rus;  Peroxidase activi ty
DOI:
10.11861/j.issn.1000-9841.1997.01.0028
文献标志码:
A
摘要:
采用SMV- a株系为毒源接种3个不同抗性类型的大豆品种(科丰1号,1138- 2和徐豆1号) ,在接种后8, 24, 48, 72小时及在3, 6, 9, 12, 15, 18天共11次连续取样与相应CK一并测定过氧化物酶的活性后发现: 未经接种的抗感品种体内PO 酶活性差异较大,感病品种中PO酶活性水平高于抗性品种2倍以上; 接种后3天内抗性品种体内PO酶活性提高快,高峰值出现早,以接种后24小时最为明显。接种后感病品种体内PO酶活性亦迅速提高,在接种后9天及15天分别出现明显高峰。同时,叶片产生典型症状。结果认为SMV侵染大豆后(尤其在接种后3天内)测定体内PO酶活性的变化,可以作为鉴定大豆对SMV抗感性的一项生化指标。
Abstract:
Peroxidase activi ty w ere tested w ith leaves of resi stant and susceptible soy bean cultivars at 3 day s interv al up to 18 days and at 24 hours interval up to 72 hours. The results show ed tha t the perox idase activi ty in suscepti le cul tiv ars was higher tha n resistantw ithout inocula tion of SMV ( CK) . The resi stant cul tiva rs af ter inoculated wi th SMV- ashow ed a n increase in peroxidase activ ity, the peak of pero xidase activi ty w as a t 24hours af ter inocuila tion wi th SMV. But the peroxidase activity increased g rea tly af ter 9days and 15 days af ter inoculated wi th SMV - a. On the susceptible cultiv ars Thi s results w ere coincided wi th symptoms development. The infected plant s w ere symptomlesso n the resi stant cultiva r.

参考文献/References:

[1 ]　庄炳昌、徐豹、廖林, 1993,接种大豆花叶病毒后,大豆叶片超氧化物歧化酶,过氧化物酶和蛋白组份的变化,植物病理学报, 23( 3): 261- 265

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[7 ]　Yan Wenzhao, Ch iu Wei fan, 1985. Ch anges of peroxidase activit y and isop eroxidases in TOM ATO plant sof di f feren t resi st ant e infected by Tobacco Mosaic Virus ( TMV) AC TA phyt opath ol ogi ca sini ca 15( 4): 193- 198
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更新日期/Last Update: 2017-01-13