[1]乔李锦,姚莉,徐冲,等.皖豫高效抗逆大豆根瘤菌Y2-4的选育[J].大豆科学,2024,43(01):45-53.[doi:10.11861/j.issn.10009841.2024.01.0045]
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皖豫高效抗逆大豆根瘤菌Y2-4的选育

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第43卷 期数: 2024年01期 页码: 45-53 栏目: 出版日期: 2024-01-20
Title:
Screening of Highly Efficient and Stress-tolerant Soybean Rhizobium Y2-4 Used in Anhui and Henan Provinces
作者:
乔李锦1; 姚莉2; 徐冲3; 4; 卢一飞3; 4; 孙安心3; 4; 秦梅3; 4; 陈文峰1
1.中国农业大学 生物学院，北京 100193；2.亳州市农业科学院，安徽 亳州 236814；3.安徽新熙盟生物科技有限公司，安徽 宿州 234000；4.安徽微壤生物科技有限公司，安徽 宿州 234000
关键词:
大豆; 根瘤菌; 共生; 高效固氮; 胁迫; 产量
DOI:
10.11861/j.issn.10009841.2024.01.0045
摘要:
为筛选适合安徽、河南两地的大豆高效抗逆根瘤菌，从安徽和河南两地选取 7 个采样点采集大豆根瘤并分离根瘤菌，通过系统发育分子鉴定、回接大豆筛选、抗逆境胁迫能力测定筛选高效抗逆大豆根瘤菌，并通过初步田间接种试验对其促生效果进行了评估。结果显示：共分离得到621株大豆根瘤细菌，其中Sinorhizobium属菌株418株（67.3%），是该地区的优势大豆根瘤菌，Bradyrhizobium属菌株198株（31.9%），Rhizobium属菌株5株（0.8%，回接大豆不能结瘤）。筛选得到1株具有应用潜力的高效抗逆根瘤菌S.fredii Y2-4，与参比菌株CCBAU 45436相比，Y2-4菌株接种的大豆叶绿素含量、根瘤数、地上干重分别提高了1.8%、15.5%和9.8%，且在3% NaCl盐胁迫及38 ℃高温胁迫条件下仍具有生长能力。田间接种试验结果显示，在全氮（225 kg·hm-2复合肥，氮磷钾比例15∶15∶15）、减氮26.6%（225 kg·hm-2复合肥，氮磷钾比例11∶15∶15）、不施氮（225 kg·hm-2复合肥，氮磷钾比例0∶15∶15）3种氮肥水平下接种Y2-4菌株均可以提高大豆产量，“减氮26.6%+接种Y2-4”处理较“全氮+不接种”处理条件下，大豆产量增加了25.5%。综上所述，Y2-4菌株可以作为皖豫两省部分地区的大豆高效抗逆根瘤菌接种菌株进行推广应用。

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更新日期/Last Update: 1900-01-01