两个杂交饲用大豆的根瘤菌生物功能与共生匹配性
- Title:
- Biological Function and Symbiotic Matching of Rhizobia in Two Hybrid Forage Soybean
- 摘要:
- 杂交饲用大豆根瘤菌具有潜在的生物功能和共生匹配性，为综合评价并筛选出最具匹配性的菌株，以野生大豆与栽培大豆的杂交后代品种“蒙农S006饲用大豆”（简称S006）和品系“8-4-1棕”（简称8-4-1）为材料，在呼和浩特市和通辽市试验种植区，于分枝期对根瘤菌进行分离纯化，经BOX-PCR分类与16S rDNA扩增测序鉴定，共得到14株根瘤菌，分别予以命名，归属于中华根瘤菌属（Sinorhizobium）、根菌属（Rhizobium）和新根瘤菌属（Neorhizobium）。采用溶磷圈法、钼锑抗比色法和Salkowski比色法对所得菌株进行溶磷能力和生长素分泌能力测定，表明14株根瘤菌均具有溶磷能力和生长素分泌能力。其中，溶磷能力最强的菌株是Z8，溶磷量为4.55 mg·L-1；生长素分泌最高的菌株是S133，分泌量为224.16 mg·L-1。在室内盆栽条件下，将所得菌株分别与试验材料进行接种，观测其共生匹配状况，以不接菌为对照。接种根瘤菌25 d后，对杂交饲用大豆地上鲜重、地上干重、株高、根瘤数、根瘤鲜重、叶片数、固氮酶活性和植株全氮含量进行测定。通过隶属函数进行综合评价，筛选出S006共生匹配效果排序前三的菌株是Z8、Z2和S134；8-4-1排序前三的菌株是Z75、S134和Z2。本研究筛选出与2个杂交饲用大豆共生匹配效果优良的菌株。后期研究中可制成根瘤菌接种菌剂，在内蒙古地区推广种植2个杂交饲用大豆时进行接种，以充分发挥优良根瘤菌的固氮作用，减少化肥施用量，为进一步深入研究提供菌种资源。
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