ZHI Ya-jing,WANG Meng,ZHEN Ya-qin,et al.Determination of Active Ingredients of Sojae Semen Praeparatum by Multi-Components with Single Marker[J].Soybean Science,2021,40(05):696-702.[doi:10.11861/j.issn.1000-9841.2021.05.0696]
淡豆豉活性成分一测多评检测方法建立
- Title:
- Determination of Active Ingredients of Sojae Semen Praeparatum by Multi-Components with Single Marker
- Keywords:
- QAMS; Sojae Semen; Relative correction factors; Quality control; External standard method; Genistin
- 文献标志码:
- A
- 摘要:
- 为快速测定淡豆豉中的主要活性成分含量，对其进行质量评价，本研究以28批不同产地淡豆豉为供试材料，建立对淡豆豉中大豆苷、黄豆黄苷、染料木苷、大豆苷元、黄豆黄素和染料木素6种活性成分含量同时测定的一测多评方法，并验证该方法在淡豆豉质量分析中的准确性与可行性。结果显示：以70%甲醇作为提取溶剂，加热回流提取60 min进行前处理，采用高效液相色谱法，以染料木苷为内参物，建立大豆苷、黄豆黄苷、大豆苷元、黄豆黄素和染料木素的一测多评相对校正因子分别为0.666，1.089，0.926，0.978和1.894。相对校正因子重现性良好，一测多评法和外标法测量结果间无明显差异。本研究建立的方法准确可行，可用于淡豆豉的质量控制。
- Abstract:
- In order to quickly determine the content of the main active ingredients in the Sojae Semen and evaluate its quality，this study used 28 batches of Sojae Semen Praeparatum from different origins as the test materials.A quantitative analysis of multi-components with single marker(QAMS) was established for the simultaneous determination of daidzin，glycitin，genistin，daidzein，glycitein，genistein in crude and processed Sojae Semen Praeparatum，which was proved to be a accurate and feasible method in the quality analysis in Sojae Semen Praeparatum.The results showed that the established relative correction factors of daidzin，glycitin，daidzein，glycitein，genistein were 0.666，1.089，0.926，0.978 and 1.894，with 70% methanol as the extraction solvent，heating and refluxing extraction for 60 min for pretreatment，and using genistin as the internal reference by using HPLC.The reproducibility of the relative correction factor was good，and there was no significant difference between the QAMS method and external standard measurement value.This method is accurate and feasible，and can be used as quality control of Sojae Semen.
参考文献/References:
［1］国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社，2020:342.(Chinese Pharmacopoeia Commission.Chinese pharmacopoeia：The first part[M].Beijing：China Medical Science Press，2020:342.)[2]陶弘景.名医别录[M].北京：中国中医药出版社，2013.(Tao H J.Famous doctors[M].Beijing：China Traditional Chinese Medicine Publishing House，2013.)[3]陈衍.宝庆本草折衷[M].北京:人民卫生出版社，1991.(Chen Y.Baoqing materia medica eclectic[M].Beijing：People’s Medical Publishing House，1991.)[4]林王敏，翁倩倩，邓爱平，等.基于文献的淡豆豉发酵过程成分转化分析[J/OL].中国中药杂志，2021:1-18.(Lin W M，Weng Q Q，Deng A P，et al.Literature-based analysis of the conversion of components in the fermentation process of Sojae Semen Praeparatum[J/OL].China Journal of Chinese Materia Medica，2021:1-18.)〖HJ1.53mm〗[5]李娜，黄庆柏.淡豆豉中的异黄酮成分及药理作用与临床应用[J].中国现代中药，2008，10(7):18.(Li N，Huang Q B.Pharmacological effects and clinical application of Sojae Semen praeparatum extract[J].Modern Chinese Medicine，2008，10(7):18.)[6]黄文娟，张艳丽，刘晶，等.淡豆豉、大豆黄卷中总异黄酮含量差异及退热作用研究[J].新疆医科大学学报，2019，42(9):1198-1201.(Huang W J，Zhang Y L，Liu J，et al.Study on the relationship between the total isoflavone content of Sojae Semen Praeparatum and Sojae Semen germinatum and antipyretic effect of rats[J].Journal of Xinjiang Medical University，2019，42(9):1198-1201.)[7]李鸷，曹冬英，许文，等.基于发酵过程的淡豆豉6种黄酮类成分质量控制研究[J].药学研究，2019，38(10):563-566，573.(Li Z，Cao D Y，Xu W，et al.Quality control of six flavonoids in Sojae Semen Praeparatum based on fermentation process[J].Journal of Pharmaceutical Research，2019，38(10)：563-566，573.)[8]张景，冯亭亭，张明柱.UPLC同时测定淡豆豉中6种异黄酮的含量[J].中药材，2016，39(11)：2563-2565.(Zhang J，Feng T T，Zhang M Z.Simultaneous determination of six isoflavones content in Sojae Semen by UPLC[J].Journal of Chinese Medicinal Materials，2016，39(11)：2563-2565.)[9]王智民，高慧敏，付雪涛，等.“一测多评”法中药质量评价模式方法学研究[J].中国中药杂志，2006(23)：1925-1928.(Wang Z M，Gao H M，Fu X T，et al.Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine[J].China Journal of Chinese Materia Medica，2006(23)：1925-1928.)[10]周园，董秋菊，冯薇，等.一测多评法测定南方菟丝子中7种活性成分的含量[J].中草药，2018，49(1)：227-232.(Zhou Y，Dong Q J，Feng W，et al.Determination of seven effective constituents in crude and processed Cuscuta australis by quantitative analysis of multi-components with single marker[J].Chinese Traditional and Herbal Drugs，2018，49(1)：227-232.)[11]张敏，吴运莉，印酬，等.“一测多评”法测定淡豆豉药材中4种黄酮类成分[J].中国药学杂志，2014，49(19)：1740-1743.(Zhang M，Wu Y L，Yin C，et al.Determination of soybean isoflavones in Semen Sojae Praeparatum by quantitative analysis of multi-components with a single-marker[J].Chinese Pharm-aceutical Journal，2014，49(19)：1740-1743.)[12]田伟，甄亚钦，王鑫国，等.一测多评法用于忍冬藤多成分含量测定的适用性研究[J].中国中医药信息杂志，2018，25(11):77-82.(Tian W，Zhen Y Q，Wang X G，et al.Applicability study on QAMS for multi-component content determination of Lonicerae Japonicae Caulis[J].Chinese Journal of Information on Traditional Chinese Medicine，2018，25(11)：77-82.)[13]匡艳辉，王德勤，徐科一，等.一测多评法测定白芍中7种成分的含量[J].中药材，2020，48(10)：2499-2530.(Kuang Y H，Wang D Q，Xu K Y，et al.Determination of seven constituents in crude and processed paeoniae alba radix by quantitative analysis of multi-components with single marker[J].Journal of Chinese Medicinal Materials，2020，48(10)：2499-2530.)[14]徐文武，谢涛，吕东峰，等.一测多评法同时测定红参中11种人参皂苷的含量[J].中草药，2021，52(7)：2099-2105.(Xu W W，Xie T，Lyu D F，et al.Simultaneous determination of 11 saponins components in red ginseng by QAMS method[J].Chinese Traditional and Herbal Drugs，2021，52(7)：2099-2105.)
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备注/Memo
收稿日期：2021-02-28