NIU Li-ying,CAI Guang-hua,WANG Xin-guo.Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins[J].Soybean Science,2010,29(01):164-167.[doi:10.11861/j.issn.1000-9841.2010.01.0164]
利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮
- Title:
- Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins
- 文章编号:
- 1000-9841(2010)01-0164-04
- Keywords:
- Semen Sojea Praeparatum; Soybean Isoflavone; Macroporous Adsorptive Resins; Adsorption; Separation
- 分类号:
- R284.2
- 文献标志码:
- A
- 摘要:
- 以染料木素为指标,采用紫外分光光度法测定异黄酮的含量,并利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮。结果表明:该树脂分离淡豆豉异黄酮的工艺参数为:上样液浓度0.225 ·mL-1,pH值4.5,最大上样量150 mL,以4 B·h-1的流速吸附,以8倍柱床体积的70%乙醇作为洗脱剂,洗脱流速 4 B·h-1,树脂可重复使用2次。经初步吸附分离,异黄酮得率达40%以上。
- Abstract:
- Use ultraviolet spectrophotometry to detect the content of soybean isoflavone,with the content of genistein as the index,to investigate the ADS-7 macroporous adsorptive resins for separation and purification of soybean isoflavone in the Semen Sojea Praeparatum. The optimized technological parameters of the ADS-7 macroporous adsorptive resins were as following:loading sample density 0.225 ·mL-1,pH 4.5,the maximum sample volume 150 mL,4 B·h-1 flow rate to adsorption and the volume of 70%(v/v)ethanol as 8 BV with desorption-flow 4 B·h-1. With these conditions,more than 40% of soybean isoflavone could be obtained from primary adsorption and purification,and the ADS-7 macroporous adsorptive resins can be used twice.
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备注/Memo
基金项目:河北省自然基金资助项目(C2009001061)。