CUI li-jian,SHI Su-qin,LIU Min-yan,et al.Determination Method of Multi-target Components in Semen Sojae Praeparatum[J].Soybean Science,2012,31(01):127-130.[doi:10.3969/j.issn.1000-9841.2012.01.029]
淡豆豉中多指标成分的含量测定方法研究
- Title:
- Determination Method of Multi-target Components in Semen Sojae Praeparatum
- 文章编号:
- 1000-9841（2012）01-0127-04
- Keywords:
- Semen Sojae Praeparatum; HPLC; UV; Total isoflavones; Daidzein; Genistein
- 分类号:
- TS201.2
- 文献标志码:
- A
- 摘要:
- 建立淡豆豉中多指标成分含量测定方法，采用HPLC法同时测定淡豆豉中大豆苷元和染料木素含量，UV法测定总异黄酮含量。色谱条件：Agilent HC-C18色谱柱(250×4.6 mm，5.0 μm)；甲醇-0.1％冰醋酸(50∶50)为流动相；流速1 mL·min-1；检测波长261 nm；柱温30℃；进样量10 μL。紫外分光光度计采用261 nm波长测定淡豆豉提取液的吸光度值。结果表明：大豆苷元和染料木素色谱峰的分离度良好，淡豆豉中大豆苷元、染料木素的保留时间分别为9.79和14.87 min，峰面积积分值与进样量呈良好的线性(r＞0.9999)，平均回收率分别为98.90%和100.53%。总异黄酮含量测定线性范围为1.44~7.20 μg·mL-1，平均回收率为102.26%。该法简便易行，能够用于评价淡豆豉质量。
- Abstract:
- To establish methods for the determination of Daidzein and Genistein in Semen Sojae Praeparatum by high performance liquid chromatography and total isoflavones by ultraviolet spectrophotometry.The established HPLC conditions were:an Agilent HC-C18 column(250×4.6 mm，5.0 μm)was applied;the mobile phase was MeOH∶0.1％HAc=50∶50;the temperature of column was 30℃;the flow rate was 1.0 mL·min-1;detective wavelength was set at 261 nm. Wavelength at 261 nm of ultraviolet spectrophotometry was adopted to determine the absorbance of samples solution.The results showed that the retention time of Daidzein and Genistein was 9.79 and 14.87 min，with the average recovery 98.90% and 100.53%，respectively.The integral value of peak area has linear relationships with sample amount of Daidzein or Genistein(r＞0.9999).Total isoflavones from 1.44 to 7.20 μg·mL-1 showed a linear relationship and the average recovery was 102.26%.The methods are simple and easy to evaluate the quality of Semen Sojae Praeparatum.
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备注/Memo
基金项目：河北省自然科学基金资助项目(C2009001061)。