WANG Tian,WANG Zhi-jie,ZHANG Yun-he,et al.Study on Relationship Between Soybean Leghemoglobin Content and Yield[J].Soybean Science,2020,39(01):45-51.[doi:10.11861/j.issn.1000-9841.2020.01.0045]
大豆根瘤豆血红蛋白含量与产量关系研究
- Title:
- Study on Relationship Between Soybean Leghemoglobin Content and Yield
- Keywords:
- Soybean; Root nodule; Leghemoglobin; Yield
- 文献标志码:
- A
- 摘要:
- 为明确大豆豆血红蛋白与产量的关系，以12个吉林省审定推广的吉农系列大豆品种为材料，在盛花期（R2）、结荚期（R4）、鼓粒期（R6）和成熟期（R8）测定根结瘤特性和根瘤中豆血红蛋白的含量测量产量，并分析不同品种根瘤数量、干重和豆血红蛋白含量变化规律及其与产量的相关关系。结果表明：吉农51、吉农39和吉农41产量处于较高水平，显著高于吉农38、吉农43和吉农45，并且在R6和R8期吉农51、吉农41和吉农39根瘤豆血红蛋白含量比其它品种高12.4%~95.7%和14.6%~88.1%。R6和R8期根瘤豆血红蛋白的含量与根瘤数量和干重呈显著正相关，且R6期相关性更大。R6和R8期根瘤干重和根瘤数量与产量均呈显著正相关，大豆根瘤豆血红蛋白含量与产量呈极显著正相关。结果说明豆血红蛋白与产量的关系密切，豆血红蛋白含量可以作为筛选高固氮结瘤能力大豆品种的指标。
- Abstract:
- In order to determine the relationship between soybean leghemoglobin content and yield, 12 Jinong varieties approved and popularized by Jilin province were taken as testing samples to determine characteristics of root nodules and leghemoglobin content in root nodules of R2, R4, R6 and R8 stages and yield. The changes of nodule number, nodule dry weight and leghemoglobin content and their relationship with yield were analyzed. The results showed that the yield of Jinong 51, Jinong 39 and Jinong 41 remained significantly higher than that of Jinong 38, Jinong 43 and Jinnong 45. Also leghemoglobin content of Jinong 51, Jinong 39 and Jinong 41 in R6 and R8 periods were respectively 12.4%-95.7% and 14.6%-88.1% higher than other varieties. The leghemoglobin content in R6 and R8 periods has a significant positive correlation with the number and dry weight of nodule, and the correlation is closer in R6 period. Both the nodule dry weight and nodule number have a significant positive correlation with yield, and leghemoglobin content have an extremely significant positive correlation in R6 and R8 periods. It indicates that leghemoglobin content is closely related to yield. This research provides a basis for studying feasibility of using leghemoglobin content as an index to screen out soybeans of strong nitrogen fixation ability.
参考文献/References:
［1］陈伟, 朱俊峰,田国强.中美贸易摩擦对中国大豆的影响及对策分析［J］. 大豆科学, 2019, 38(1): 118-123. (Cheng W, Zhu J F, Tian G Q. The impact and countermeasures analysis of Sino-US trade friction on China′s soybean［J］. Soybean Science, 2019, 38(1): 118-123.)［2］ 唐海龙. 有机肥与化肥配施对土壤环境质量影响的研究［D］. 泰安: 山东农业大学, 2012. (Tang H L. Research on the efficiency of organic and chemical fertilizers to soil environmental quality［D］. Taian: Shandong Agricultural University, 2012.)［3］ 张武, 杨琳, 王紫娟. 生物固氮的研究进展及发展趋势［J］. 云南农业大学学报(自然科学), 2015, 30(5): 810-821. (Zhang W, Yang L, Wang Z J. Advance and development trend of biological nitrogen fixation research［J］. Journal of Yunnan Agricultural University (Natural Science), 2015,30(5): 810-821.)［4］ 关大伟,李力,岳现录, 等.我国大豆的生物固氮潜力研究［J］.植物营养与肥料学报,2014,20(6):1497-1504. (Guan D W, Li L, Yue X L, et al. Study on potential of biological nitrogen fixation of soybean in China［J］. Plant Nutrition and Fertilizer Science, 2014,20(6):1497-1504.)［5］ Gabriel S, Lucas B, Fernando S, et al. Relative importance of biological nitrogen fixation and mineral uptake in high yielding soybean cultivars［J］. Plant and Soil, 2017, 418: 191-203.［6］ 喻定文,任晓伟,高凡,等.StNHX1基因过表达对盐胁迫下菜用大豆结瘤固氮的影响［J］.河北农业大学学报, 2019,42(1):22-27. (Yu D W, Ren X W, Gao F, et al. Effects of overexpression of StNHX1 gene on nodulation and nitrogen fixation in vegetable soybean under salt stress［J］. Journal of Hebei Agricultural University,2019,42(1):22-27.)［7］ 柯丹霞, 彭昆鹏. 利用酵母双杂交系统筛选大豆结瘤因子受体NFR1α的互作蛋白［J］.作物学报，2020，46（1）：31-39.(Ke D X, Peng K P. Screening of NFR1α-interactive proteins in soybean using yeast two hybrid system［J］. Acta Agronomica Sinica, 2020,46(1):31-39.)［8］ 赵亚兰,尉亚辉. 豆血红蛋白的研究进展［J］.西北植物学报,2000, 30(4): 684-689.（Zhao Y L, Wei Y H. Progress in the study of leghemoglobin［J］. Acta Botanica Boreali-Occidentalia Sinica,2000, 30(4):684-689.）［9］ 王树起,韩晓增,乔云发,等.缺磷条件下低分子量有机酸对大豆氮积累和结瘤固氮的影响［J］.应用生态学报,2009,20(5):1079-1084. (Wang S Q, Han X Z, Qiao Y F, et al. Effects of low molecular organic acids on nitrogen accumulation, nodulation, and nitrogen fixation of soybean (Glycine max L.) under phosphorus deficiency stress［J］. Chinese Journal of Applied Ecology,2009,20(5):1079-1084.)［10］左元梅,刘永秀,张福锁.与玉米混作改善花生铁营养对其根瘤形态结构及豆血红蛋白含量的影响［J］.植物生理与分子生物学学报,2003, 29(1):33-38. (Zuo Y M, Liu Y X, Zhang F S. Effects of improvement of iron nutrition by mixed cropping with maize on nodule microstructure and leghaemoglobin content of 〖HJ1.88mm〗peanut［J］. Journal of Plant Physiology and Molecular Biology, 2003, 29(1):33-38.)［11］Ji Y M, Luo R P, Zhao Z G, et al. Effects of different nitrogen application rates and methods on root nodule growth and yield of soybean［J］. Agricultural Science and Technology,2018,19(2): 67-72.)［12］赵宏伟,李秋祝,魏永霞.不同生育时期干旱对大豆主要生理参数及产量的影响［J］.大豆科学,2006, 25(3): 329-332. (Zhao H W, Li Q Z, Wei Y X. Effect of drought at different growth stages on main physiological parameters and yield in soybean［J］. Soybean Science, 2006, 25(3): 329-332.)［13］白子裕, 刘冰, 徐晨, 等. 不同类型大豆根瘤氨同化关键酶活性比较［J］.分子植物育种,2019,17(18):6097-6103. (Bai Z Y, Liu B, Xu C, et al. Comparison of key enzyme activities of ammonia assimilation in different types of soybean root nodules［J］. Molecular Plant Breeding,2019,17(18):6097-6103.)［14］郭荫杰. 大豆豆血红蛋白还原酶（GmFLbR）基因的图位克隆和功能初步分析［D］.哈尔滨: 中国科学院大学(中国科学院东北地理与农业生态研究所), 2018. (Guo Y J. The ferric hemoglobin reductase (GmFLbR) gene of soybean mapping and preliminary function analysis［D］. Harbin: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 2018.)［15］严君,韩晓增,王守宇,等.不同施氮量及供氮方式对大豆根瘤生长及固氮的影响［J］.江苏农业学报,2010,26(1):75-79. (Yan J, Han X Z, Wang S Y, et al. Effects of different n supply levels and methods on nodule growth and nitrogen fixation in soybean (Glycine max L.) ［J］. Jiangsu Journal of Agricultural Sciences,2010,26(1):75-79.)［16］杨美英,王乾钦,赵洪锟,等.不同蛋白质含量大豆结瘤特性与根瘤氮代谢物含量的比较分析［J］.中国油料作物学报,2011,33(5): 492-498. (Yang M Y, Wang Q Q, Zhao H K, et al. Comparison and analysis of nodular growth characteristics and nitrogenous compounds in nodule among soybean species with different protein content［J］. Chinese Journal of Oil Crop Sciences,2011,33(5): 492-498.)［17］姜妍,王清泉,李远明,等.施氮水平对7S亚基缺失大豆根系形态和结瘤固氮的影响［J］.大豆科学, 2017,36(2):267-273.(Jiang Y, Wang Q Q, Li Y M, et al. Effect of different nitrogen application levels on the root morphology, nodulation and nitrogen fixation in 7S subunit lacked soybean［J］. Soybean Science,2017,36(2):267-273.）［18］姚琳,徐克章,张治安,等.吉林省不同年代育成大豆品种根瘤数量、鲜重和体积的变化［J］.中国油料作物学报,2009,31(2):196-201. (Yao L, Xu K Z, Zhang Z A, et al. Nodule number fresh weight and volume of soybean cultivars over the years in Jilin province［J］. Chinese Journal of Oil Crop Sciences,2009,31(2):196-201.)［19］郑永美,杜连涛,王春晓,等.不同花生品种根瘤固氮特点及其与产量的关系［J］.应用生态学报,2019,30(3):961-968. (Zheng Y M, Du L T, Wang C X, et al. Nitrogen fixation characteristics of root nodules in different peanut varieties and their relationship with yield［J］. Chinese Journal of Applied Ecology,2019,30(3):961-968.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]张立军,孙旭刚,王昌陵,等.盆栽条件下水肥调控对大豆生长和产量的影响[J].大豆科学,2014,33(03):398.[doi:10.11861/j.issn.1000-9841.2014.03.0398]
ZHANG Li-jun,SUN Xu-gang,WANG Chang-ling,et al.Regulation Effect of Water and Fertilizer on Growth and Yield in Soybean under Pot Experiment Condition[J].Soybean Science,2014,33(01):398.[doi:10.11861/j.issn.1000-9841.2014.03.0398]
[12]闫春娟,王文斌,孙旭刚,等.水肥互作对大豆生理特性、根瘤固氮及产量的影响[J].大豆科学,2011,30(02):229.[doi:10.11861/j.issn.1000-9841.2011.02.0229]
YAN Chun-juan,WANG Wen-bin,SUN Xu-gang,et al.Effect of Water-Fertilizer Interaction on Physiological Characteristics，Nitrogen Fixation and Yield of Soybean[J].Soybean Science,2011,30(01):229.[doi:10.11861/j.issn.1000-9841.2011.02.0229]
[13]乔云发,韩晓增.长期定量施肥对大豆根系形态和根瘤性状的影响[J].大豆科学,2011,30(01):119.[doi:10.11861/j.issn.1000-9841.2011.01.0119]
QIAO Yun-fa,HAN Xiao-zeng.Effects of Long-term Fertilization on Root Phenotype and Nodulation of Soybean[J].Soybean Science,2011,30(01):119.[doi:10.11861/j.issn.1000-9841.2011.01.0119]
[14]于晓波,向达兵,杨文钰,等.结瘤性状不同基因型大豆对接种花生根瘤菌Spr2-9的响应[J].大豆科学,2010,29(01):41.[doi:10.11861/j.issn.1000-9841.2010.01.0041]
YU Xiao-bo,XIANG Da-bing,YANG Wen-yu,et al.Response of Soybean Genotypes with Different Nodulation Traits to Peanut Rhizobia Spr2-9 Inoculation[J].Soybean Science,2010,29(01):41.[doi:10.11861/j.issn.1000-9841.2010.01.0041]
[15]严君,韩晓增,王守宇,等.不同形态氮对大豆根瘤生长及固氮的影响[J].大豆科学,2009,28(04):674.[doi:10.11861/j.issn.1000-9841.2009.04.0674]
YAN Jun,HAN Xiao-zeng,WANG Shou-yu,et al.Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean (Glycine max L.)[J].Soybean Science,2009,28(01):674.[doi:10.11861/j.issn.1000-9841.2009.04.0674]
[16]蒋跃林 张庆国 张仕定 岳 伟 陈庭甫 樊丽莉.大气 CO 2 浓度升高对大豆根瘤量及其固氮活性的影响＊[J].大豆科学,2006,25(01):53.[doi:10.11861/j.issn.1000-9841.2006.01.0053]
Jiang Yuelin Zhang Qingguo Zhang Shiding Yue Wei Chen Tinfu Fan Lili.EFFECTOF ELEVATED ATMOSPHERIC CO 2 CONCENTRATION ONROOT NODULES AND NITROGENASE ACTIVITY IN SOYBEAN[J].Soybean Science,2006,25(01):53.[doi:10.11861/j.issn.1000-9841.2006.01.0053]
[17]於丙军 刘友良.大豆耐盐性研究进展[J].大豆科学,2000,19(02):154.[doi:10.11861/j.issn.1000-9841.2000.02.0154]
Yu BingjunLiu Yo uliang.RECENT PROGRESS IN STUDY ON SALT TOLERANCE IN SOYBEAN[J].Soybean Science,2000,19(01):154.[doi:10.11861/j.issn.1000-9841.2000.02.0154]
[18]张明才 何钟佩 田晓莉 王保民 段留生 翟志席 李召虎.植物生长调节剂BR 和SHK -6 对大豆生物产量和根瘤固氮活性的激素调控研究1[J].大豆科学,2004,23(02):96.[doi:10.11861/j.issn.1000-9841.2004.02.0096]
Zhang Ming cai He Zhongpei Tian Xiaoli Wang Baomin Duan liusheng Zhai zhixi Li Zhaohu.HORMONAL REGULATION OF PLANT GROWTH REGULATOR BR ANDSHK-6 ON SOYBEAN BIOMASS AND NITROGENASE ACTIVITY[J].Soybean Science,2004,23(01):96.[doi:10.11861/j.issn.1000-9841.2004.02.0096]
[19]甘银波 陈静 Ineke Stulen.大豆不同生长阶段施用氮肥对生长、结瘤及产量的影响[J].大豆科学,1997,16(02):125.[doi:10.11861/j.issn.1000-9841.1997.02.0125]
Gan Yinbo Chen Jing Ineke Stulen.EFFECTS OF N APPLTCATION AT DTFFERENT GROWTH STAGES ON GROWTHNODULATIONAND YTELD OF SOYBEANS[J].Soybean Science,1997,16(01):125.[doi:10.11861/j.issn.1000-9841.1997.02.0125]
[20]姬月梅,罗瑞萍,赵志刚,等.不同施氮量及施氮方式对大豆根瘤生长及产量的影响[J].大豆科学,2017,36(06):887.[doi:10.11861/j.issn.1000-9841.2017.06.0887]
JI Yue-mei,LUO Rui-ping,ZHAO Zhi-gang,et al.Effects of Different Nitrogen Application and Methods on Nodule Growth and Yield of Soybean (Glycine max L.)[J].Soybean Science,2017,36(01):887.[doi:10.11861/j.issn.1000-9841.2017.06.0887]
备注/Memo
收稿日期：2019-07-25