ZHANG Xiong,XU Lu-rong,LIU Si-cen,et al.Transcriptome Analysis of Soybean Leaves Under the Stress of Pseudomonas syringae[J].Soybean Science,2019,38(06):879-888.[doi:10.11861/j.issn.1000-9841.2019.06.0879]
丁香假单胞菌变种Pto（AvrB）胁迫下大豆叶片转录组分析
- Title:
- Transcriptome Analysis of Soybean Leaves Under the Stress of Pseudomonas syringae
- Keywords:
- Soybean; Pseudomonas syringae; Pto（AvrB）; RNA-seq; Differential expressed genes
- 摘要:
- AvrB是丁香假单胞杆菌大豆致病变种分泌的III型效应蛋白因子之一，为研究携带异源效应蛋白AvrB的丁香假单胞菌番茄致病变种Pto（AvrB）侵染大豆后的基因转录变化情况，以大豆Williams 82为试验材料，采用RNA-seq技术对不同菌株处理的大豆叶片进行基因表达谱差异分析，探索大豆非寄主病原菌Pseudomonas syringae pv. tomato携带的异源效应蛋白AvrB是否可以增加非寄主病原菌的致病性。结果显示：共得到43 422个序列信息，不同处理间共有序列为37 611个，其中Pto（AvrB）处理组的序列信息最多。根据GO功能分析可以将基因根据功能分为分子功能、细胞成分和生物学过程3类，其差异表达基因涉及信息传递、免疫、次生代谢等过程。KEGG 通路富集分析表明，差异表达基因主要富集在植物激素信号转导、异黄酮生物合成、苯丙氨酸代谢、植物-病原体相互作用等通路中。在RT-PCR检测中发现4个随机挑选的差异表达基因的表达量与RNA-Seq的变化趋势一致，说明RNA-Seq的结果可靠。研究结果有助于阐明III型效应蛋白因子促进病原菌致病效应的机理，从而为分析AvrB在病原菌与大豆互作过程中的毒性功能研究和培育抗病大豆品种提供依据。
- Abstract:
- AvrB is one of the type III effector proteins secreted by Pseudomonas syringae pv. Glycinea, in order to study the changes of gene expression profile of soybean under biotic stress, we analyzed the difference of gene expression profiles of soybean leaves treated by different strains of Pto and Pto(AvrB)with RNA-seq technologyinsoybean Williams 82. And we explored whether the heterologous effector protein AvrB carried by the non-host pathogen Pseudomonas syringae pv. tomato can increase the pathogenicity of non-host pathogens.A total of 43 422 unigenes were screened out,37 611 common sequences between different treatments were coincident, and the AvrB treat group has the most sequence information. According to GO function analysis, genes can be divided into three major categories: Molecular function, cellular composition and biological process. The differentially expressed genes(DEGs) are widely involved in processes such as information transmission, immunity, and secondary metabolism. The KEGG pathway enrichment analysis indicated that the differentially expressed genes were mainly enriched in pathways such as plant hormone signal transduction, isoflavone biosynthesis, phenylalanine metabolism, and plant-pathogen interaction. The expression levels of four randomly selected DEGs in the RT-PCR were in conformity with the RNA-Seq data, confirming the reliability of the RNA-Seq results.The research promotes us to understand the pathogenic mechanism of type III effector protein factor promoting pathogenic bacteria，andprovides a basisforcultivating disease-resistant soybean varieties.
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备注/Memo
基金项目：上海市国际科技合作基金（17310742000）；上海交通大学“AgriX”基金（AgriX 2016007）。第一作者简介：〖HTSS〗〖ZK（〗张雄（1995），男，硕士，主要从事植物生物胁迫下抗性机制研究。Email：ZHANGXIONG233333@sjtueducn。〖ZK）〗〖HT6H〗通讯作者：〖HTSS〗〖ZK（〗耿雪青（1977），女，博士，副研究员，主要从事植物生物胁迫下抗性机制研究。Email：xqgeng@sjtueducn。