HUANG Jian-li,DENG Su-shuang,SHEN Jia-cheng,et al.Identification and Response to Phytophthora sojae Infections of JAZ Gene Family in Soybean[J].Soybean Science,2019,38(06):868-878.[doi:10.11861/j.issn.1000-9841.2019.06.0868]
大豆JAZ基因家族的鉴定及其对疫霉胁迫的响应
- Title:
- Identification and Response to Phytophthora sojae Infections of JAZ Gene Family in Soybean
- Keywords:
- Soybean; JAZ gene family; Bioinformatics; Phytophthora sojae; qRT-PCR
- 摘要:
- JAZ（Jasmonate ZIM-domain）蛋白是茉莉酸信号途径中重要的负调控因子，对植物的防御反应具有重要意义。然而，鲜有关于大豆JAZ基因的报道，为了揭示大豆中JAZ基因家族的特征和潜在功能，本研究利用生物信息学方法从大豆基因组中鉴定到24个JAZ基因，分别命名为GmJAZ1~GmJAZ24，并对这24个基因进行基因结构分析、保守基序分析、系统进化树构建、染色体定位、启动子顺式作用元件分析以及大豆疫霉菌胁迫下的响应分析。结果表明：（1）24个GmJAZs基因不均匀的分布在大豆的14条染色体上，其中9号染色体上分布的数目最多，为4个；（2）大豆、拟南芥和水稻的JAZ基因家族可分成5个亚组（C1~C5），其中大豆与拟南芥的JAZ基因亲缘关系最近；（3）该家族成员大多含有响应茉莉酸和脱落酸等激素的顺式作用元件，少数含有响应逆境胁迫的顺式作用元件；（4）大豆疫霉菌处理后，C4亚组成员显著上调表达，C1亚组成员微弱上调，而C2、C3和C5亚组成员下调表达，表明大豆JAZ基因家族成员对大豆疫霉菌的胁迫具有不同的响应模式。
- Abstract:
- JAZ (Jasmonate ZIM-domain) protein, a key transcriptional repressor in JA signaling pathway, plays an important role in plant defense. However, there is limited information about this family. In order to study the characteristic and potential function of the JAZ gene family in soybean, this study identified a total of 24 JAZ genes in the soybean genome by bioinformatics, named as GmJAZ1to GmJAZ24. We extensively analyzed intron-exon distribution and protein motif organization, built phylogenetic tree, and analyzed chromosome localization, cis-acting regulatory elements and response to Phytophthora sojae infections for JAZ family in soybean. The results showed that a total of 24 GmJAZs was unevenly distributed on the fourteen chromosomes with maximum number of genes (four JAZs) on chromosome 9. The JAZ family divided into five clusters (C1~C5) including soybean, Arabidopsis and rice in the phylogenetic tree, The JAZ genes of soybean were the most closely related to Arabidopsis. Promoter analysis revealed that most of soybean JAZ genes contained jasmonic acid (JA) and abscisic acid (ABA) responsive cis-acting elements, and some with defense and stress related cis-acting elements. After Phytophthora sojae infections, C4 subgroup members were significantly upregulated, C1 subgroup members were weakly upregulated, while the C2, C3 and C5 subgroup members were downregulated, which indicated that the soybean JAZ genes exhibited divergent expression patterns in response to Phytophthora sojae treatment. The results of the present study provide valuable information for functional characterization of GmJAZ in response to Phytophthora sojae in soybean.
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备注/Memo
基金项目：国家重点研发计划（2017YFD0101500）；转基因生物新品种培育重大专项（2016ZX08004002）；现代农业产业技术体系建设专项（CARS-004-PS10）；中央高校基本科研业务费专项资金（KYT201801，Y0201700146）；长江学者和创新团队发展计划（PCSIRT_17R55）；江苏省现代作物生产协同创新中心。第一作者简介：黄建丽（1994-），女，硕士，主要从事大豆分子遗传育种研究。E-mail: 2017101136@njau.edu.cn。通讯作者：郭娜（1984-），女，博士，副教授，主要从事大豆分子遗传研究。E-mail: guona@njau.edu.cn；邢邯（1963-），男，博士，教授，主要从事菜用大豆育种与大豆分子遗传研究。E-mail: hanx@njau.edu.cn。