CHEN Jin-ling,XU Yuan,CHEN Yu-mei,et al.Sequencing Analysis of Transcriptome During the Different Developmental Stages in Soybean Seed[J].Soybean Science,2019,38(04):533-541.[doi:10.11861/j.issn.1000-9841.2019.04.0533]
大豆籽粒不同发育时期的转录组分析
- Title:
- Sequencing Analysis of Transcriptome During the Different Developmental Stages in Soybean Seed
- 文献标志码:
- A
- 摘要:
- 为了从分子水平上研究大豆籽粒不同发育时期的油脂合成与累积机理,对大豆开花20 d(DD_20)、30 d(DD_30)、40 d(DD_40)、50 d(DD_50)的籽粒进行了转录组测序。通过对转录组测序数据的分析,共得到原始数据461 566 988条,经过滤获得开花后20,30,40和50 d的clean reads,分别为107 548 920,111 670 776,109 339 672和108 884 270条。DD_20/DD_30、DD_30/DD_40、DD_40/DD_50比较组中的差异表达基因分别为4 759,6 245和13 763个,其中上调基因分别为1 801,2 941和5 695个。差异表达基因的KEGG pathway分析中,分别得到134,133和136条代谢通路,筛选到8个与油脂合成相关的差异表达基因,ACC、FATB、GPAT、DGAT1、G3PDH、KASI、SAD和FAD2。研究结果对深入研究大豆籽粒脂质合成的调控机理及大豆高油育种具有重要参考价值。
- Abstract:
- In order to study the mechanism of oil synthesis and accumulation in soybean seeds at different developmental stages at the molecular level, the soybean seeds of 20 d (DD_20), 30 d (DD_30), 40 d (DD_40) and 50 d (DD_50) were used as test materials in transcriptome sequencing. Through the analysis of transcriptome sequencing data, 461 566 988 raw reads were obtained. After filtration, the clean reads of DD_20, DD_30, DD_40 and DD_50 were obtained respectively, as 107 548 920, 111 670 776, 109 339 672 and 108 884 270. The total differential expression genes of DD_20/DD_30, DD_30/DD_40, DD_40/DD_50 were 4 759, 6 245 and 13 763 respectively, of which up-regulated genes were 1 801, 2 941 and 5 695 respectively. KEGG pathway analysis of differential expression genes obtained 134, 133 and 136 KEGG pathways, screened 8 differential expression genes related to lipid synthesis, as ACC, FATB, GPAT, DGAT1, G3PDH, KASI, SAD and FAD2. The results provide an important reference for further study on the regulation mechanism of soybean seed lipid synthesis, as well as for the breeding of high oil content soybean varieties.
参考文献/References:
[1]吕慧颖, 王道文, 葛毅强, 等. 大豆育种行业创新动态[J]. 植物遗传资源学报, 2018, 19(3): 464-467. (Lyu H Y, Wang D W, Ge Y Q, et al. Innovation of soybean breeding industry[J]. Journal of Plant Genetic Resources, 2018, 19(3): 464-467.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]姚敏磊,张璟曜,周汐,等.大豆响应低磷胁迫的数字基因表达谱分析[J].大豆科学,2016,35(02):213.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
YAO Min-lei,ZHANG Jing-yao,ZHOU Xi,et al.The Digital Gene Expression Profiling Analysis of Genes in Response to Low Phosphorus Stress in Soybean[J].Soybean Science,2016,35(04):213.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
[12]张雄,徐鲁荣,刘思岑,等.丁香假单胞菌变种Pto(AvrB)胁迫下大豆叶片转录组分析[J].大豆科学,2019,38(06):879.[doi:10.11861/j.issn.1000-9841.2019.06.0879]
ZHANG Xiong,XU Lu-rong,LIU Si-cen,et al.Transcriptome Analysis of Soybean Leaves Under the Stress of Pseudomonas syringae[J].Soybean Science,2019,38(04):879.[doi:10.11861/j.issn.1000-9841.2019.06.0879]
备注/Memo
收稿日期:2019-01-21