[1]杨柳,于翠梅,刘铭,等.农杆菌介导大豆遗传转化影响因素研究进展[J].大豆科学,2018,37(05):803-806.[doi:10.11861/j.issn.1000-9841.2018.05.0803]
 YANG Liu,YU Cui-mei,LIU Ming,et al.Research Advances of Agrobacterium-mediated Transformation Affecting Factors in Soybean[J].Soybean Science,2018,37(05):803-806.[doi:10.11861/j.issn.1000-9841.2018.05.0803]
点击复制

农杆菌介导大豆遗传转化影响因素研究进展

参考文献/References:

[1]Hartman G L, West E D, Herman T K. Crops that feed the world 2.Soybean-worldwide production, use, and constraints caused by pathogens and pests[J]. Food Security, 2011, 3(1): 5-17.
[2]Lam H M, Xu X, Liu X, et al. Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection[J]. Nature Genetics, 2010, 42(12): 1053-1059.
[3]Olhoft P M, Donovan C M, Somers D A. Soybean (Glycine max) transformation using mature cotyledonary node explants[J]. Methods in Molecular Biology, 2006, 343: 385-396.
[4]Yamada T, Takagi K, Ishimoto M. Recent advances in soybean transformation and their application to molecular breeding and genomic analysis[J]. Breeding Science, 2012, 61(5): 480-494.
[5]侯文胜, 林抗雪, 陈普, 等. 大豆规模化转基因技术体系的构建及其应用[J]. 中国农业科学, 2014, 47(21): 4198-4210. (Hou W S, Lin K X, Chen P, et al. Establishment and prospect of efficient transformation systems for soybean[J]. Scientia Agricultura Sinica, 2014, 47(21): 4198-4210.)
[6]Olhoft P M, Flagel L E, Donovan C M, et al. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method[J]. Planta, 2003, 216(5): 723-735.
[7]Paz M M, Shou H, Guo Z, et al. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant[J]. Euphytica, 2004, 136(2): 167-179.
[8]Paz M, Martinez J A, Fonger T, et al. Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation[J]. Plant Cell Reports, 2006, 25(3): 206-213.
[9]Yamada T, Watanabe S, Arai M, et al. Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean[J]. Plant Tissue Culture Letters, 2010, 27(2): 217-220.
[10]Zhang F, Chen C, Ge H, et al. Efficient soybean regeneration and Agrobacterium-mediated transformation using a whole cotyledonary node as an explant[J]. Biotechnology and Applied Biochemistry, 2014, 61(5): 620-625.
[11]Arun M, Subramanyam K, Mariashibu T S, et al. Application of sonication in combination with vacuum infiltration enhances the Agrobacterium-mediated genetic transformation in Indian soybean cultivars[J]. Applied Biochemistry and Biotechnology, 2015, 175(4): 2266-2287.
[12]Yang X F, Yu X Q, Zhou Z, et al. A high-efficiency Agrobacterium tumefaciens, mediated transformation system using cotyledonary node as explants in soybean (Glycine max L.)[J]. Acta Physiologiae Plantarum, 2016, 38(3): 1-10.
[13]Gelvin S B.Agrobacterium-mediated plant transformation: The biology behind the ‘gene-jockeying’ tool[J]. Microbiology and Molecular Biology Reviews Mmbr, 2003, 67(1): 16-37.
[14]Hinchee M A W, Connorward D V, Newell C A, et al. Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer[J]. Nature Biotechnology, 1988, 6(6): 915-922.
[15]Meurer C A, Dinkins R D, Collins G B. Factors affecting soybean cotyledonary node transformation[J]. Plant Cell Reports, 1998, 18(3-4): 180-186.
[16]王凤敏, 李涛, 王运杰, 等. 影响农杆菌介导大豆子叶节遗传转化因素的研究[J]. 大豆科学, 2011, 30(4): 557-562. (Wang F M, Li T, Wang Y J, et al. Assessment of factors affecting soybean cotyledonary-node Agrobacterium-mediated genetic transformation[J]. Soybean Science, 2011, 30(4): 557-562.)
[17]薄路花, 曹越平. 不同大豆品种对农杆菌EHA105和GV3101敏感性及共培养条件的优化[J]. 上海交通大学学报(农业科学版), 2015, 33(1): 26-31. (Bo L H, Cao Y P. Sensitivity of different soybean genotype to Agrobacterium EHA105 and GV3101 and optimization of cocultivation conditions[J]. Journal of Shanghai Jiaotong University(Agricultural Science), 2015, 33(1): 26-31.)
[18]Lacroix B, Tzfira T, Vainstein A, et al. A case of promiscuity: Agrobacterium′s endless hunt for new partners[J]. Trends in Genetics Tig, 2006, 22(1): 29-37.
[19]Gelvin S B. Traversing the cell:Agrobacterium T-DNA′s journey to the host genome[J]. Frontiers in Plant Science, 2012, 4(1): 27-32.
[20]邹智.农杆菌vir基因诱导因子研究进展[J].中国生物工程杂志,2011,31(7):126-132.(Zou Z.Advances on factors influencing induction of Agrobacterium tumefaciens virulence genes[J].China Biotechnology,2011,31(7):126-132.)
[21]Zhang Y M, Zhang H M, Liu Z H, et al. Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction[J]. Plant Cell Tissue and Organ Culture, 2015, 121(1): 1-11.
[22]Zhang Y M, Liu Z H, Yang R J, et al. Improvement of soybean transformation via Agrobacterium tumefaciens, methods involving α-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis[J]. Plant Cell Reports, 2016, 35(6): 1259-1271.
[23]Arun M, Chinnathambi A, Subramanyam K, et al. Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean[J]. Biotech, 2016 6(2): 148:1-12.
[24]赵晓雯,吴芳芳,狄少康,等. 农杆菌介导的大豆子叶节遗传转化技术流程及操作要点[J].大豆科学, 2011, 30(3): 362-368. (Zhao X W,Wu F F,Di S K, et al. Technique flow and key operation points of Agrobacterium- mediated genetic transformation of soybean cotyledonary node[J].Soybean Science,2011, 30(3): 362-368.)
[25]吴国栋, 修宇,王华芳. 优化子叶节转化法培育大豆Mt DREB2A转基因植株.植物学报[J], 2018, 53 (1): 59-71.(Wu G D, Xiu Y, Wang H F. Breeding of Mt DREB2A transgenic soybean by an optimized cotyledonary-node method[J]. Chinese Bulletin of Botany,2018, 53 (1): 59-71.)
[26]Li S X, Cong Y H, Liu Y P,et al. Optimization of Agrobacterium-mediated transformation in soybean[J]. Frontiers in Plant Science,2017,8:1-15.
[27]贾钰莹, 蒋滢, 赵强, 等. 农杆菌介导超高产大豆子叶节遗传转化研究[J]. 大豆科学, 2014, 33(5): 634-637. (Jia Y Y, Jiang Y, Zhao Q, et al. Study on Agrobacterium-mediated transformation system of super-high-yielding soybean cotyledon node[J]. Soybean Science, 2014, 33(5): 634-637.)
[28]Brencic A, Winans S C. Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria[J]. Microbiology and Molecular Biology Reviews, 2005, 69(1): 155-194.
[29]Gelvin S B. Integration of Agrobacterium T-DNA into the plant genome[J]. Annual Review of Genetics, 2017, 40(1): 195-217.
[30]Ziemienowicz A.Agrobacterium-mediated plant transformation: Factors, applications and recent advances[J]. Biocatalysis and Agricultural Biotechnology, 2014, 3(4): 95-102.
[31]Huang F C, Fu B J, Liu Y T, et al. Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 participate in Agrobacterium-mediated plant transformation[J]. International Journal of Molecular Sciences, 2018, 19(3): 638-659.
[32]Bourras S, Rouxel T, Meyer M.Agrobacterium tumefaciens gene transfer: How a plant pathogen hacks the nuclei of plant and nonplant organisms[J]. Phytopathology, 2015, 105(10): 1288-1301.
[33]Garcíacano E, Hak H, Magori S, et al. The Agrobacterium F-box protein effector VirF destabilizes the Arabidopsis GLABROUS1 enhancer/binding protein-like transcription factor VFP4, a transcriptional activator of defense response genes[J]. Molecular Plant-Microbe Interactions, 2018, DOI: 10.1094/MPMI-07-17-0188-FI.
[34]Donaldson P A, Simmonds D H. Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean[J]. Plant Cell Reports, 2000, 19(5): 478-484.
[35]王罡, 王萍, 蔺宇, 等. 大豆基因型对根癌农杆菌菌株敏感性的研究[J]. 遗传, 2002, 24(3): 297-300. (Wang G, Wang P, Lin Y, et al. The studies of sensitivity of genotype in soybean to lines of Agrobacterium tumefaciens[J]. Hereditas, 2002, 24(3): 297-300.)
[36]李文霞, 宁海龙, 吕文河, 等. 农杆菌介导大豆子叶节转化系统的优化[J]. 中国农业科学, 2008, 41(4): 971-977. (Li W X, Ning H L, Lyu W H, et al. Optimization of the Agrobacterium-mediated transformation systems of soybean cotyledonary node[J]. Scientia Agricultura Sinica, 2008, 41(4): 971-977.)
[37]Song Z Y, Tian J L, Fu W Z, et al. Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability[J]. Journal of Zhejiang University-Science B(Biomedicine and Biotechnology), 2013, 14: 289-298.
[38]Jia Y Y, Yao X D, Zhao M Z, et al. Comparison of soybean transformation efficiency and plant factors affecting transformation during the Agrobacterium infection process[J]. International Journal of Molecular Sciences, 2015, 16(8): 18522-18543.
[39]Parrott W A, Williams E G, Hildebrand D F, et al. Effect of genotype on somatic embyrogenesis from immature cotyledons of soybean[J]. Plant Cell Tissue and Organ Culture, 1989, 16(1): 15-21.
[40]Trick H N, Finer J J. Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue[J]. Plant Cell Reports, 1998, 17(6-7): 482-488.
[41]Liu H K, Yang C, Wei Z M. Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system[J]. Planta, 2004, 219(6): 1042-1049.
[42]Hong H P, Zhang H, Olhoft P, et al. Organogenic callus, as the target for plant regeneration and transformation via Agrobacterium, in soybean (Glycine max, (L.) Merr.)[J]. In Vitro Cellular and Developmental Biology-Plant, 2007, 43(6): 558-568.
[43]王爽, 郭兵福, 张丽娟, 等. 农杆菌介导大豆子叶节转化法外植体选择新方法[J]. 大豆科学, 2016, 35(5): 723-729. (Wang S, Guo B F, Zhang L J, et al. New method for the selection of explants in the Agrobacterium mediated cotyledon nodes transformation in soybean[J]. Soybean Science, 2016, 35(5): 723-729.)
[44]龚一富, 高峰. 根癌农杆菌感染对甘薯外植体生理生化特性的影响[J]. 中国农业科学, 2008, 41(6): 1649-1654. (Gong Y F, Gao F. Effects of Agrobacterium tumefaciens on the physiological and biochemical characters in sweet potato explants[J]. Scientia Agricultura Sinica, 2008, 41(6): 1649-1654.)
[45]Olhoft P M, Somers D A. L-cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells[J]. Plant Cell Reports, 2001, 20, 706-711.
[46]Olhoft P M, Lin K, Galbraith J, et al. The role of thiol compounds in increasing Agrobacterium-mediated transformation of soybean cotyledonary-node cells[J]. Plant Cell Reports, 2001, 20, 731-737.
[47]Ditt R F, Nester E W, Comai L. Plant gene expression response to Agrobacterium tumefaciens[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(19): 10954-10959.
[48]Tie W, Zhou F, Wang L, et al. Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: Lessons from transformation assays and genome-wide expression profiling[J]. Plant Molecular Biology, 2012, 78(1-2): 1-18.
[49]Zhou X, Wang K,Lyu D, et al. Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens[J]. Plos One, 2013, 8(11): e79390.
[50]Creelman R A, Mullet J E. Jasmonic acid distribution and action in plants:Regulation during development and response to biotic and abiotic stress[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92(10): 4114-4119.
[51]Mariashibu T S, Subramanyam K, Arun M, et al. Vacuum infiltration enhances the Agrobacterium-mediated genetic transformation in Indian soybean cultivars[J]. Acta Physiologiae Plantarum, 2013, 35(1): 41-54.
[52]Guo B F, Yong G, Wang J, et al. Co-treatment with surfactant and sonication signifi cantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean[J]. Journal of Integrative Agriculture, 2015, 14(7): 1242-1250.
[53]Wang G L, Xu Y N. Hypocotyl based Agrobacterium mediated transformation of soybean(Glycine max) and application for RNA interference[J]. Plant Cell Reports, 2008, 27(7): 1177-1184.
[54]李艳超, 赵青松, 王凤敏, 等. 大豆遗传转化技术研究进展[J]. 大豆科学, 2015, 34(1): 155-162. (Li Y C, Zhao Q S, Wang F M, et al. Recent advances of soybean transformation[J]. Soybean Science, 2015, 34(1): 155-162.)
[55]李桂兰, 刘晨光, 乔潇, 等. 共培养条件对农杆菌转化大豆子叶节的影响[J]. 核农学报, 2014, 28(9): 1567-1575. (Li G L, Liu C G, Qiao X, et al. Conditions of co-culture affecting on the efficiency of Agrobacterium-mediated transformation of cotyledonary node of soybean[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(9): 1567-1575.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
 LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
 LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
 WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
 ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
 LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
 DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
 ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
 GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
 WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
 LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]胡倩倩,方 星,王建国,等.硼营养对大豆组织再生及农杆菌介导的遗传转化效率的影响[J].大豆科学,2013,32(04):445.[doi:10.11861/j.issn.1000-9841.2013.04.0445]
 HU Qian-qian,FANG Xing,WANG Jian-guo,et al.Effect of Boron on Explant Regeneration and Frequency of Agrobacterium-mediated Transformation in Soybean[J].Soybean Science,2013,32(05):445.[doi:10.11861/j.issn.1000-9841.2013.04.0445]
[12]张庆林,赵艳,张艳,等.不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究[J].大豆科学,2011,30(04):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
 ZHANG Qing-lin,ZHAO Yan,ZHANG Yan,et al.Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105[J].Soybean Science,2011,30(05):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
[13]李永光,李冬梅,李维娜,等.基于不定芽原位诱导的大豆转基因方法[J].大豆科学,2012,31(02):163.[doi:10.3969/j.issn.1000-9841.2012.02.001]
 LI Yong-guang,LI Dong-mei,LI Wei-na,et al.A New Soybean Transformation Method Based on Adventitious Shoots Induction[J].Soybean Science,2012,31(05):163.[doi:10.3969/j.issn.1000-9841.2012.02.001]
[14]郝荣华,邵 群,杨素欣,等.根癌农杆菌介导的大豆子叶节转化体系的优化[J].大豆科学,2012,31(02):167.[doi:10.3969/j.issn.1000-9841.2012.02.002]
 HAO Rong-hua,SHAO Qun,YANG Su-xin,et al.Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node[J].Soybean Science,2012,31(05):167.[doi:10.3969/j.issn.1000-9841.2012.02.002]
[15]邱波,王志坤,孟凡立,等.不同大豆基因型再生性及对农杆菌敏感性的研究[J].大豆科学,2011,30(05):752.[doi:10.11861/j.issn.1000-9841.2011.05.0752]
 QIU Bo,WANG Zhing-kun,MENG Fan-li,et al.Regeneration and Sensitivity to Agrobacterium of Different Soybean Genotypes[J].Soybean Science,2011,30(05):752.[doi:10.11861/j.issn.1000-9841.2011.05.0752]
[16]李海芬,刘庆梅,杨光宇,等.硼酸诱导的大豆子叶节转化新方法[J].大豆科学,2011,30(02):194.[doi:10.11861/j.issn.1000-9841.2011.02.0194]
 LI Hai-fen,LIU Qing-mei,YNAG Guang-yu,et al.Boric Acid Mediated Transformation of Soybean (Glycine max) Cotyledonary Node[J].Soybean Science,2011,30(05):194.[doi:10.11861/j.issn.1000-9841.2011.02.0194]
[17]王萍,高世庆,郭永来,等.利用农杆菌介导将抗逆相关基因GmDREB导入大豆的研究[J].大豆科学,2008,27(01):47.[doi:10.11861/j.issn.1000-9841.2008.01.0047]
 WANG Ping,GAO Shi-qing,GUO Yong-lai,et al.Transformation of Stress Resistance Related Gene GmDREB into Soybean via Agrobacterium-mediation[J].Soybean Science,2008,27(05):47.[doi:10.11861/j.issn.1000-9841.2008.01.0047]
[18]王全伟,张海玲,白晶,等.农杆菌介导的大豆植株整体转化[J].大豆科学,2008,27(02):190.[doi:10.11861/j.issn.1000-9841.2008.02.0190]
 WANG Quan-wei,ZHANG Hai-ling,BAI Jing,et al.Agrobacterium-Mediated Plant Transformation of Soybean[J].Soybean Science,2008,27(05):190.[doi:10.11861/j.issn.1000-9841.2008.02.0190]
[19]李卉,武天龙.CaCl2诱导大豆花粉管通道农杆菌转基因研究[J].大豆科学,2007,26(01):55.[doi:10.3969/j.issn.1000-9841.2007.01.013]
 LI Hui,WU Tian-long.TRANSFOMING AGROBACTERIUM INTO SOYBEAN BY MEANS OF POLLEN TUBE PATHWAY INDUCED BY CaCl2[J].Soybean Science,2007,26(05):55.[doi:10.3969/j.issn.1000-9841.2007.01.013]
[20]方星,胡倩倩,王建国,等.大豆悬浮细胞培养及作为外源基因转化受体的研究[J].大豆科学,2015,34(01):36.[doi:10.11861/j.issn.1000-9841.2015.01.0036]
 FANG Xing,HU Qian-qian,WANG Jian-guo,et al.Culture and Exogenous Gene Transformation of Suspension Cells in Soybean[J].Soybean Science,2015,34(05):36.[doi:10.11861/j.issn.1000-9841.2015.01.0036]

备注/Memo

收稿日期:2018-04-19

基金项目:辽宁省教育厅项目(L2015490);辽宁省科技厅项目(2015020762)。
第一作者简介:杨柳(1992-),女,硕士,主要从事大豆转基因研究。E-mail:948753955@qq.com。
通讯作者:于翠梅(1974-),女,博士,副教授,主要从事大豆高产育种及转基因研究。E-mail:yucuimei@163.com。

更新日期/Last Update: 2018-10-08