LUO Jian,FENG Lei,LI Dongmei,et al.Comparative Analysis of Vitamin E Content Accumulation in Different Soybean Varieties[J].Soybean Science,2017,36(02):250-256.[doi:10.11861/j.issn.1000-98412017020250]
不同大豆品种籽粒维生素E含量积累比较分析
- Title:
- Comparative Analysis of Vitamin E Content Accumulation in Different Soybean Varieties
- Keywords:
- Soybean; Vitamin E; Tocopherol; High performance liquid chromatography
- 文献标志码:
- A
- 摘要:
- 采用高效液相色谱技术法(HPLC)以东北三省及内蒙古大豆产区的180份种质资源为研究材料,检测其籽粒总维生素E含量及各组分含量,进而分析维生素E总含量及各组分含量间的相关性,选用代表性大豆品种在不同生育时期维生素E积累动态进行分析。结果表明:大豆种质资源籽粒维生素E积累是从R5期开始,初期积累量呈上升趋势,R6期积累量开始大幅度减少,但从R6至R8期积累量又呈现大幅度上升趋势,在R8期积累量趋于稳定。经筛选合丰50和L21为高维生素E含量品种,黑秣食豆和茶秣食豆为低维生素E含量品种,代表性大豆品种籽粒维生素E含量差异主要体现在R6~R7时期,该时期最终决定代表性大豆品种最终维生素E含量,这为研究高维生素E大豆新品种选育提供了理论基础,同时为高维生素E相关基因位点和候选基因的挖掘奠定了表型鉴定基础。
- Abstract:
- In this study, high performance liquid chromatography(HPLC) was used to study the content of total vitamin E and the content of each component in 180 soybean germplasm resources which from the three provinces of Northeast China and Inner Mongolia The content of total vitamin E and the content of each component were analyzed The dynamic analysis of vitamin E accumulation in representative soybean cultivars at different growth stages was analyzed The results showed that the accumulation of Vitamia E began to increase from the beginning of R5, the accumulation at R6 began to decrease, but the accumulation showed a significant upward trend from R6 to R8, The accumulation trends to be stable at R8 Hefeng 50 and L 21 were high content of vitamin E cultivars, and the content of vitamin E in the Heimoshidou and Chamoshidou were low content of vitamin E cultivars The difference of vitamin E content in grains was mainly reflected in R6-R7 period, which ultimately determined the final vitamin E content, which provided a theoretical basis for the research of high vitamin E soybean variety breeding and also laid the basis of the high Vitamin E related gene site and candidate genes for phenotypic identification
参考文献/References:
[1]宋晓燕,杨天奎天然维生素E的功能及应用[J].中国油脂,2000,25(6):45-47(Song X Y, Yang T K Function and application of natural vitamin E[J] China Oil,2000, 25(6):45-47)?
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备注/Memo
基金项目: 国家自然基金(31201227,31301339,31671717);国家"十二五"科技支撑计划(2011BAD35B061);国家现代农业产业技术体系(CAR-04-PS04);黑龙江省教育厅科学研究项目(12521008)。