[1]韩英鹏,赵雪,高赛男,等.大豆花叶病毒病种粒斑驳抗性基因定位[J].大豆科学,2016,35(04):696-698.[doi:10.11861/j.issn.1000-9841.2016.04.0696]
　HAN Ying-peng,ZHAO Xue,GAO Sai-nan,et al.Mapping of Gene with Ｒesistance to Seed Coat Mottle in Soybean[J].Soybean Science,2016,35(04):696-698.[doi:10.11861/j.issn.1000-9841.2016.04.0696]
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大豆花叶病毒病种粒斑驳抗性基因定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 696-698 栏目: 出版日期: 2016-07-20
Title:
Mapping of Gene with Ｒesistance to Seed Coat Mottle in Soybean
作者:
韩英鹏; 赵雪; 高赛男; 李海燕; 李文滨
( 东北农业大学大豆生物学教育部重点实验室/农业部北方大豆生物学与遗传育种区域重点实验室，黑龙江哈尔滨150030)
Author(s):
HAN Ying-peng; ZHAO Xue; GAO Sai-nan; LI Hai-yan; LI Wen-bin
( Northeast Agricultural University，Chinese Education Ministry’s Key Laboratory of Soybean Biology / Key Laboratory of Northeastern Soybean Biologyand Breeding ( Genetics) of Chinese Agriculture Ministry，Harbin 150030，China)
关键词:
大豆;  花叶病毒病;  抗性基因定位;  分子辅助选择;  种粒斑驳
Keywords:
Soybean;  Soybean mosaic virus;  Mapping of resistance gene;  Molecular assistant selection;  Seed coat mottle
分类号:
S511
DOI:
10.11861/j.issn.1000-9841.2016.04.0696
文献标志码:
A
摘要:
大豆种粒斑驳严重影响大豆商品性，选育抗病品种可有效控制大豆种粒斑驳。利用抗源东农93-046 与品1246 所衍生的F8∶9代群体，对其进行种粒斑驳鉴定，同时利用分子标记对其抗性基因进行初步分析，结果表明: 以斑驳率5%为界限划分抗感株系，F8∶9代抗病株系数与感病株系数基本符合1∶ 1 的比例，这表明东农93-046 的种粒斑驳抗性受一对等位基因控制。根据前人构建的一个包括13 个分子标记F 连锁群的遗传连锁图谱，结合单标记和复合区间作图法将东农93-046 抗性基因位点定位于SSＲ 分子标记Satt114 附近。
Abstract:
Soybean seed coat mottle seriously affects on soybean commodity． The selection of resistant varieties could efficientlycontrol this disease． The aim of this study is to map the resistant gene of resisance cultivar Dongnong 93-046 through a F8∶9populations from a cross between Dongnong 93-046 and Pin 1246． The results showed the ratio ( resistance line number: susceptibleline number) was 1∶1 in the F8∶9 generation，which suggested that the resistance of Donong 93-046 was controlled bya pair of alleles． A total of 13 SSＲ markers in linkage group F were used to construct a genetic map． Ｒesistance gene ofDongnong 93-046 was located near the SSＲ marker Satt114 through single marker analysis and composite interval analysis． Itoffered a good foundation for molecular marker assisted selection．

参考文献/References:

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备注/Memo

基金项目: 黑龙江省普通高等学校新世纪优秀人才培养计划( 1253-NCET-005) 。第一作者简介: 韩英鹏( 1978-) ，男，教授，博导，主要从事大豆遗传育种研究。E-mail: hyp234286@ aliyun． com。通讯作者: 李文滨( 1958-) ，男，教授，博导，主要从事大豆遗传育种研究和分子生物技术研究。E-mail: wenbinli@ yahoo． com。

更新日期/Last Update: 2016-08-23