[1]李凯,智海剑.大豆对大豆花叶病毒病抗性的研究进展[J].大豆科学,2016,35(04):525-530.[doi:10.11861/j.issn.1000-9841.2016.04.0525]
　LI Kai,ZHI Hai-jian.Advances in Ｒesistance to Soybean Mosaic Virus Disease in Soybean[J].Soybean Science,2016,35(04):525-530.[doi:10.11861/j.issn.1000-9841.2016.04.0525]
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大豆对大豆花叶病毒病抗性的研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 525-530 栏目: 出版日期: 2016-07-20
Title:
Advances in Ｒesistance to Soybean Mosaic Virus Disease in Soybean
作者:
李凯； 智海剑；
Author(s):
LI Kai; ZHI Hai-jian
( Soybean Ｒesearch Institute of Nanjing Agricultural University /Key Laboratory of Biology and Genetic Improvement of Soybean，Ministry of Agriculture /National Center for Soybean Improvement /National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing 210095，China)
关键词:
大豆;  大豆花叶病毒;  株系划分;  抗病基因;  转基因
Keywords:
Soybean;  Soybean mosaic virus( SMV) ;  Classification of SMV strains;  Ｒesistance gene;  Transgenic
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0525
文献标志码:
A
摘要:
大豆花叶病毒病是我国大豆生产上的最主要病害之一,在我国东北、黄淮及南方大豆产区普遍发生,严重降低大豆产量和品质。我国不同学者曾将江苏、东北、湖北及山东等地的SMV株系做过局部划分。为促进大豆抗病育种研究成果的交流和种质的交换,南京农业大学国家大豆改良中心从不同类型种质资源和国内外鉴别系统中筛选出10个鉴别能力强、反应稳定的鉴别寄主,将我国大豆产区25个省(市)的SMV划分为22个株系;通过不同病毒分离物的全基因组序列分析,发现大豆花叶病毒和菜豆普通花叶病毒基因组重组形成的SMV新类型;从种质资源中发掘出广谱抗源科丰1号和齐黄1号等优异抗源;发现其对15个株系的抗侵染作用分别由单显性基因控制;将15个抗侵染基因定位在大豆2、6、13和14号染色体上,完成Rsc3、Rsc4、Rsc6、Rsc7、Rsc8、Rsc13、Rsc14、Rsc15、Rsc17、Rsc18等抗性基因的精细定位,发现抗性基因成簇存在;证明抗病、系统坏死、系统花叶3类症状由一组复等位基因控制;发现大豆对SMV存在数量抗性(抗扩展),它由一对加性主基因+加性-显性多基因共同控制;利用分子标记辅助选择,聚合3个抗源品种、3条染色体上的多个抗性基因,创造了兼抗20个株系的新种质;利用基因干扰原理,完成了SMV基因HC-Pro和CP及隐性大豆抗病基因eIF4E的遗传转化,在阳性后代中获得一批优异抗性材料。
Abstract:
Soybean mosaic virus( SMV) disease is one of the most common and destructive viral diseases in soybean productionin China，such as in Northeastern China，Huanghuai valleys and Southern China． It declines the soybean yield and degradesthe soybean seed quality. Earlier，several SMV strain differential systems have been established in Jiangsu，Hubei，Shandong provinces and Northeastern China. National center for soybean improvement( NCSI) ，Nanjing Agricultural University，China has been actively working on SMV research for the past decades. To facilitate exchange of information of resistancesources and nation wide utilization of the resistant soybean cultivars，researchers from NCSI established the nationwide uniformSMV strain classification system using 10 different resistant soybean cultivars. Based on this strain classification system，twenty-two SMV strains have been identified from 25 provinces of China. Several new SMV types，for instance recombination withSMV and bean common mosaic virus( BCMV) were also identified and confirmed by complete genome sequencing of variousSMV isolates. We collected the soybean cultivars，landraces from China and other countries and screened against SMV strainsas well as identified resistance sources such as Kefeng 1，Qihuang 1 and Dabaima，etc. Our further investigation showed thatsingle dominant gene confers the resistance to 15 SMV strains and they were mapped on Chromosomes 2，6，13 and 14. Amongthem，we fine mapped the Ｒsc3，Ｒsc4，Ｒsc6，Ｒsc7，Ｒsc8，Ｒsc13，Ｒsc14，Ｒsc15，Ｒsc17 and Ｒsc18 genes and found resistantgenes are always clusters. And we confirmed that 3 types of symptoms i. e. resistance，necrosis and mosaic to SMV in soybean，were conditioned by a set of multiple alleles. The studies have demonstrated that the mechanisms of resistance in infectionand in development to SMV are different. The resistance in development was controlled by one additive major gene plusadditive and dominant polygenes. New broad spectrum resistant soybean germplasms，which can resistant to 20 SMV strains，were developed by marker assisted selection( MAS) using 3 soybean cultivars containing multiple resistant genes. In additionwe completed the genetic transformation of SMV genes such as HC-Pro，CP and a recessive soybean resistance genes eIF4Ebased on gene interference principle and a set of excellent resistant material were obtained in the progeny of positive transgenicsoybean materials.

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备注/Memo

基金项目: 国家自然科学基金( 31371646，31571690) ; 中央高校基本科研业务费( Y0201600115) ; 国家现代农业产业技术体系( CAＲS-04) ;转基因生物新品种培育科技重大专项( 2016ZX08004-004) ; 江苏省现代作物生产协同创新中心( JCIC-MCP) 。第一作者简介: 李凯( 1979-) ，男，博士，副教授，主要从事大豆抗病遗传育种研究。E-mail: kail@ njau. edu. cn。通讯作者: 智海剑( 1957-) ，男，教授，博导，主要从事大豆抗病遗传育种研究。E-mail: zhj@ njau. edu. cn。

更新日期/Last Update: 2016-08-19