[1]张慧,于仁敬,邹佳男,等.大豆细菌性病害病原菌分离鉴定与致病性评价[J].大豆科学,2016,35(01):112-117,123.[doi:10.11861/j.issn.1000-9841.2016.01.0112]
　ZHANG Hui,YU Ren-jing,ZOU Jia-nan,et al.Isolation, Identification and Pathogenicity Evaluation of Soybean Bacterial Disease Pathogens[J].Soybean Science,2016,35(01):112-117,123.[doi:10.11861/j.issn.1000-9841.2016.01.0112]
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大豆细菌性病害病原菌分离鉴定与致病性评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 112-117,123 栏目: 出版日期: 2016-01-20
Title:
Isolation, Identification and Pathogenicity Evaluation of Soybean Bacterial Disease Pathogens
作者:
张慧1; 于仁敬1; 邹佳男1; 辛大伟1; 胡振帮1; 张勇3; 刘春燕2; 陈庆山1
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
?2.黑龙江省农垦科研育种中心，黑龙江 哈尔滨 150090；
?3.黑龙江省农业科学院 克山分院，黑龙江 克山 161606
Author(s):
ZHANG Hui1;  YU Ren-jing1;  ZOU Jia-nan1;  XIN Da-wei1;  HU Zhen-bang1;  ZHANG Yong3;  LIU Chun-yan2;  CHEN Qing-shan1
1.College of Agricultural Northeast Agricultural University, Harbin 150030, China ;
2. Land Reclamation Research and Breeding Center of Heilongjiang, Harbin 150090, China；
3. Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161606，China
关键词:
大豆; 细菌性病原菌; 分离; 鉴定; 致病性
Keywords:
Soybean;  Bacterial pathogen;  Isolation;  Identification;  Pathogenicity
DOI:
10.11861/j.issn.1000-9841.2016.01.0112
文献标志码:
A
摘要:
对在黑龙江哈尔滨闫家岗农场采集的绥农14大豆细菌性斑点病叶片进行病原菌分离，将分离得到的大豆细菌性斑点病病原菌进行抗生素耐性鉴定、分子鉴定并对大豆品种回接试验，分离得到1个细菌菌株。经过革兰氏染色、病原菌回接试验、16S rDNA分子鉴定方法确定分离的菌株为丁香假单胞杆菌属，命名为Psgneau 2。分离的菌株对终浓度为25，50，100 μg·mL-1的氨苄青霉素和羧苄青霉素都具有耐性，对壮观青霉素、利福平、卡那霉素、庆大霉素、四环素和氯霉素不具有抗性。采用高压喷雾和菌落滴度的方法将分离的Psgneau 2菌株接种10个大豆品种，进行致病性鉴定，结果表明绥农14极易感病，合丰35抗病性高于其它品种
Abstract:
In order to research soybean bacterial disease in Heilongjiang province, one bacterial pathogen was isolated from Suinong 14 diseased leaves which were collected from Harbin Yanjiagang farm. The pathogen has been carried out antibiotic resistance identification, molecular identification, re-inoculated identification and pathogenicity identification. By Gram staining, pathogen-back test, 16S rDNA molecular identification, the pathogen was Pseudomonas syringae and named Psgneau 2. The pathogen exhibited resistance against two antibiotics, ampicillin and carbenicillin, as three concentration 25，50，100 μg·mL-1, but susceptible to spectinomycin, rifampicin, kanamycin, gentamicin, tetracycline and chloramphenicol. Ten soybean varieties were inoculated with Psgneau 2, of which Suinong 14 was more susceptible than others and Hefeng 35 was the most resistant to Psgneau 2.

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备注/Memo

基金项目：国家自然科学基金（31471516，31271747）；黑龙江省高校长江后备支持计划（2014CJHB004）。

第一作者简介：张慧（1989-），女，硕士，主要从事大豆抗病机制研究。E-mail：dnnxzhanghui@163.com。
通讯作者：陈庆山（1973-），男，博士，教授，主要从事大豆生物技术研究。E-mail：qshchen@126.com；刘春燕（1981-），女，博士，副研究员，主要从事大豆抗病机制研究。E-mail：cyliucn@126.com。

更新日期/Last Update: 2016-02-05