[1]陆亮,贺建波,苗龙,等.大豆GmDGK7和GmTPR基因与油脂相关性状的关联分析[J].大豆科学,2015,34(06):938-944.[doi:10.11861/j.issn.1000-9841.2015.06.0938]
　LU Liang,HE Jian-bo,MIAO Long,et al.Association of GmDGK7 and GmTPR Genes with Oil Related Traits in Soybean[J].Soybean Science,2015,34(06):938-944.[doi:10.11861/j.issn.1000-9841.2015.06.0938]
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大豆GmDGK7和GmTPR基因与油脂相关性状的关联分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 938-944 栏目: 出版日期: 2015-12-25
Title:
Association of GmDGK7 and GmTPR Genes with Oil Related Traits in Soybean
作者:
陆亮; 贺建波; 苗龙; 王晓婷; 刘方东; 王新通; 盖钧镒; 李艳
南京农业 大学国家大豆改良中心/作物遗传与种质创新国家重点实验室/农业部大豆生物学与遗传育种重点实验室，江苏 南京 210095
Author(s):
LU Liang;  HE Jian-bo;  MIAO Long;  WANG Xiao-ting;  LIU Fang-dong;  WANG Xin-tong;  GAI Jun-yi;  LI Yan
National Center for Soybean Improvement /National Key Laboratory of Crop Genetics and Germplasm Enhancement / MOA Key Laboratory for Biology and Genetic Improvement of Soybean, Nanjing Agricultural University, Nanjing 210095, China
关键词:
大豆; 脂肪酸; 单核苷酸多态性; 功能分子标记。
Keywords:
Soybean;  Fatty acids;  Single nucleotide polymorphism;  Functional molecular markers
DOI:
10.11861/j.issn.1000-9841.2015.06.0938
文献标志码:
A
摘要:
根据已知油脂相关同源基因分析，选择两个油脂代谢相关基因，分别编码甘油二酯激酶7(diacylglycerol kinase 7，DGK7)和TPR蛋白(pentatricopeptide repeat-containing protein，TPR)，并根据两个基因内部及非翻译区（untranslated region，UTR）的SNP变异位置和突变类型，选取位于GmDGK7基因5′-UTR区的一个单核苷酸变异（SNP1）及位于GmTPR基因外显子区的SNP2设计dCAPS标记。对200份育成大豆品种的SNP1和SNP2分型及其与大豆籽粒油脂相关性状的关联分析发现，GmDGK7基因的SNP1与油脂含量显著关联（P = 6.21×10-5），GmTPR基因的SNP2与油脂含量、硬脂酸、油酸和亚油酸均显著关联（P值分别为4.9×10-11，1.6×10-6，3.4×10-5，4.09×10-8）。此外还筛选出14份高油脂（＞23%）和5份高油酸（＞33%）大豆种质，这些分子标记和种质资源可为改良大豆油脂性状和分子育种提供技术和材料基础
Abstract:
Soybean is the most important oil crop in the world, which oil production accounts for more than a quarter of global vegetable oil production, thus increasing the oil content and oil composition in soybean seed is essential for soybean breeding.However, there are limited molecular markers derived from functional genes in soybean breeding program.Two soybean genes that are homologous to known proteins involved in oil metabolic pathways were chosen to develop molecular markers in this study, including genes encoding a diacylglycerol kinase 7 (DGK7) and a pentatricopeptide repeat-containing protein (TPR), respectively. Based on the position and type of mutation of the single nucleotide polymorphisms (SNPs) within these two genes, we selected SNP1 located in the 5′-untranslated region (5′-UTR) of GmDGK7 and SNP2 located in the GmTPR exon to design dCAPS markers.Association mapping of SNP1 and SNP2 with the oil related traits among 200 soybean released cultivars showed that SNP1 in GmDGK7 is significantly (P = 6.21×10-5) associated with oil content, SNP2 in GmTPR is significantly associated with the content of oil, stearic acid, oleic acid and linoleic acid (P values were 4.9×10-11, 1.6×10-6, 3.4×10-5?and 4.09×10-8, respectively).Fourteen soybean cultivars with high oil content (＞ 23%) and 5 cultivars with high oleic acid (＞ 33%) were identified in this mapping population.These cultivars and dCAPS markers could be used in marker-assisted breeding to improve soybean oil content and quality.

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备注/Memo

基金项目：国家重点基础研究发展计划(“973计划”)(2011CB1093)；教育部长江学者和创新团队发展计划（PCSIRT13073）；农业部大豆生物学与遗传育种创新团队；江苏省双创计划；江苏省现代作物生产协同创新中心项目。

第一作者简介：陆亮（1989-），硕士，主要从事大豆遗传育种研究。E-mail: 2568682.ll@163.com。
通讯作者：李艳（1975-），教授，从事大豆遗传育种及基因组学研究。E-mail: yanli1@njau.edu.cn。

更新日期/Last Update: 2015-12-30