[1]问涛,焦学磊,刘晓英,等.光谱分布对大豆子叶节再生的影响[J].大豆科学,2015,34(05):826-832.[doi:10.11861/j.issn.1000-9841.2015.05.0826]
　WEN Tao,JIAO Xue-lei,LIU Xiao-ying,et al.Effects of Light Spectra on Soybean Cotyledonary Node Regeneration System[J].Soybean Science,2015,34(05):826-832.[doi:10.11861/j.issn.1000-9841.2015.05.0826]
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光谱分布对大豆子叶节再生的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年05期 页码: 826-832 栏目: 出版日期: 2015-10-25
Title:
Effects of Light Spectra on Soybean Cotyledonary Node Regeneration System
作者:
问涛; 焦学磊; 刘晓英; 徐志刚
南京农业大学 农学院，江苏 南京 210095
Author(s):
WEN Tao;  JIAO Xue-lei;  LIU Xiao-ying;  XU Zhing-gang
College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
关键词:
大豆; 光谱分布; 子叶节; 丛生芽; LED
Keywords:
Soybean;  Light spectra;  Cotyledonary node;  Multiple shoot;  LED light
DOI:
10.11861/j.issn.1000-9841.2015.05.0826
文献标志码:
A
摘要:
以辽鲜1号大豆品种为试材，研究了不同光谱分布对大豆子叶节再生的影响，为菜用大豆子叶节遗传转化受体体系的改进提供借鉴。以萌发7 d的子叶节为外植体，依次接种于丛生芽诱导培养基、伸长培养基和生根培养基中，并且分别置于6种LED组合光源中培育，以荧光灯作为对照。光密度设置为50 μmol.m-2.s-1，光周期设置为12 h.d-1。结果表明：复合光谱中630 nm红光会抑制丛生芽之间相互伸长，而660 nm红光能显著缓解相互抑制作用，并且更有利于丛生芽形态生长、叶绿素合成和干物质积累以及生根苗形态生长、干物质积累和根系发育。添加绿光或黄光均抑制丛生芽相互之间的伸长，且添加黄光抑制更强，添加绿光可促进丛生芽的叶绿素合成、干物质积累及生根壮苗。
Abstract:
The soybean cultivar Liaoxian 1 was used as the experimental material to determine the effects of different light spectra on soybean cotyledonary node regeneration. Under six different light conditions, the soybean cotyledonary nodes from germinated seeds for 7 d were used as the explant and inoculated onto shoot initiation, shoot elongation and rooting medium successively. The photosynthetic photon flux density (PPFD) was 50 μmol.m-2.s-1, and photoperiod was 12 h light/12 h dark.The results showed that the 630 nm red light resulted in inhibition of elongation between multiple shoots.Compared with 630 nm red light, 660 nm red light promoted remission of this inhibition noticeably, and resulted in greater growth, chorophyll accumulation and dry mass of multiple shoots, and greater growth, dry mass and root development of regenerated plantlet. Green or yellow light added to the complex spectra of red and blue light resulted in inhibition of elongation between multiple shoots, yellow light aggravated this inhibition. Green light added to the complex spectra of red and blue light resulted in greater growth, chorophyll accumulation and dry mass of multiple shoots, and rooting of soybean plantlet in vitro

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备注/Memo

基金项目：农业部公益行业（农业）科研专项（201303108）；国家高技术研究发展计划“863计划”（2013AA103003）。

第一作者简介：问涛 (1990-)，女，硕士，主要从事植物光生物学研究。E-mail: 2012101056@njau.edu.cn。 通讯作者：徐志刚 (1967-)，男，教授，主要从事植物光生物学与设施光环境的研究。E-mail: xuzhigang@njau.edu.cn。

更新日期/Last Update: 2015-11-07