[1]李娜,王丽娜,金勋,等.不同品种大豆叶片表皮蜡质提取后的扫描电镜观察[J].大豆科学,2015,34(03):540-544.[doi:10.11861/j.issn.1000-9841.2015.03.0540]
　LI Na,WANG Li-na,JIN Xun,et al.SEM Observation after Extraction of Epicuticular Wax on Soybean Leaves[J].Soybean Science,2015,34(03):540-544.[doi:10.11861/j.issn.1000-9841.2015.03.0540]
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不同品种大豆叶片表皮蜡质提取后的扫描电镜观察

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 540-544 栏目: 出版日期: 2015-06-25
Title:
SEM Observation after Extraction of Epicuticular Wax on Soybean Leaves
作者:
李娜; 王丽娜; 金勋; 芮海英; 潘红丽; 金铃; 李泽宇; 于吉东
黑龙江省农业科学院 大庆分院，黑龙江 大庆 163316
Author(s):
LI Na;  WANG Li-na; JIN Xun;  RUI Hai-ying;  PAN Hong-li;  JIN Ling;  LI Ze-yu;  YU Ji-dong
Daqing Branch of Heilongjiang Academy of Agricultural Sciences，Daqing 163316，China
关键词:
大豆; 表皮蜡质; 氯仿; 正己烷; 扫描电镜
Keywords:
Soybean; Epicuticular wax; Chloroform; n hexane; SEM
DOI:
10.11861/j.issn.1000-9841.2015.03.0540
文献标志码:
A
摘要:
以抗线11和黑农48两个大豆品种的成熟叶片为材料，用氯仿和正己烷两种溶剂，采用常温法和加热法提取大豆叶片表皮蜡质，对脱蜡后的叶片进行扫描电镜观察。结果显示加热法效果好于常温法。采用常温法，两种溶剂都不能将大豆叶片表皮蜡质提取干净，仍有少量絮状或颗粒状蜡质残留，但氯仿的提取效果好于正己烷；采用加热法，两种溶剂不同处理时间均能将叶片表皮蜡质完全提取干净，处理时间对蜡质的提取结果有明显影响，加热30 s时叶片表皮细胞均发生不同程度的变形。通过扫描电镜观察得出以氯仿为溶剂，加热60℃、处理时间15 s为大豆叶片简单可行的脱蜡方法。
Abstract:
Extraction of epicuticular wax on mature soybean leaves（Kangxian 11 and Heinong 48） are studied comparatively with solution CHCl3and solution n-hexane with room-temperature method and high-temperature method.The microstructure observed by scanning electronic microscope (SEM) showed that high-temperature method was better than room-temperature method. Epicuticular wax was not completely extracted with room temperature method with both solution CHCl3?and solution n-hexane, there was still a small amount of flocculent or granular waxy residue, but the extraction effect of chloroform was better than n-hexane. Epicuticular wax was completely extracted with high-temperature method with both solution CHCl3?and solution n-hexane, extraction time was found significantly affecting the extracted wax quality, high-temperature treatment 30 s, leaf epidermal cells had undergone varying degrees of deformation.In the present study, 60℃, 15 s with solution CHCl3?was the effective and simple extraction method for epicuticular wax on soybean leaves by SEM.

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备注/Memo

第一作者简介：李娜（1983-），女，硕士，研究实习员，主要从事生物技术研究。E-mail:linashmilu@126.com。

通讯作者：金勋（1962-），男，博士，副研究员，主要从事植物遗传育种研究。E-mail:jinxun6268@126.com。

更新日期/Last Update: 2015-07-16