[1]赵 雪,孙明明,韩英鹏,等.大豆种质资源耐疫霉根腐病优异等位变异挖掘[J].大豆科学,2014,33(04):488-491.[doi:10.11861/j.issn.1000-9841.2014.04.0488]
　ZHAO Xue,SUN Ming-ming,HAN Ying-peng,et al.Identification of Loci Underlying Tolerance to Phytophthora Root Rot in Soybean Germplasm[J].Soybean Science,2014,33(04):488-491.[doi:10.11861/j.issn.1000-9841.2014.04.0488]
点击复制

大豆种质资源耐疫霉根腐病优异等位变异挖掘

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年04期 页码: 488-491 栏目: 出版日期: 2014-08-25
Title:
Identification of Loci Underlying Tolerance to Phytophthora Root Rot in Soybean Germplasm
作者:
赵 雪1; 孙明明2; 韩英鹏1; 李修平3; 张洪建4; 滕卫丽1; 李文滨1
1.东北农业大学大豆生物学教育部重点实验室，农业部北方大豆生物学与遗传育种区域重点实验室,黑龙江 哈尔滨 150030；
2.黑龙江省农业科学院信息中心，黑龙江 哈尔滨 150086；
3.佳木斯大学 生命科学学院，黑龙江 佳木斯 154007；
4.黑龙江省红星农场，黑龙江 北安 164022
Author(s):
ZHAO Xue1; SUN Ming-ming2; HAN Ying-peng1; LI Xiu-ping3; ZHANG Hong-jian4; TENG Wei-li1; LI Wen-bin1
1.Key Laboratory of Soybean Biology in Chinese Ministry of Education，Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China;
2.Information Center of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;
3.College of Life Sciences,Jiamusi University,Jiamusi 154007,China;4.Hongxing Farm of Heilongjiang Province,Beian 164022,China
关键词:
大豆; 疫霉根腐病; 耐病性; 关联分析
Keywords:
Soybean; Phytophthora root rot; Tolerance to disease; Association analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.04.0488
文献标志码:
A
摘要:
利用片层接种法对全国54份大豆种质资源的大豆疫霉根腐病耐病性进行评价，获得耐疫霉根腐病大豆种质4份，中度耐病种质12份，进一步通过Solexa测序获得5 000个高质量SNP标记，并通过关联分析的方法对耐病优异等位位点的挖掘进行初步探索，挖掘到与大豆疫霉根腐病相关联的SNP标记34个，通过等位基因评价，获得31个优异等位变异，本研究结果将为大豆耐疫霉根腐病分子育种提供理论依据和基础材料。
Abstract:
A total of 54 soybean varieties(lines)were used to evaluate the tolerance to PRR and was sequenced by solexa for genotyping.For evaluation of tolerance to PRR,four tolerant accessions and twelve moderate tolerant accessions were identified.Five thousand SNP markers were generated from 54 soybean accessions and a total of 34 association signal related to tolerance to PRR were detected under GLM model.Of them,31 beneficial alleles were obtained.These results will facilitate soybean molecular breeding in PRR tolerance.

参考文献/References:

[1]沈崇尧,苏彦纯.中国大豆疫霉病菌的发现及初步研究[J].植物病理学报,1991,21(3):298.(Shen C Y,Su Y C.Discovery and preliminary studies of phytophthora megasperma?on soybean in China[J].Plant Physiology Journal,1991,21(23):298.)

[2]Jee Hyeongjin.Occurance of phytophthora?root rot on soybean(Glycine max)and identification of the causal fungus[J].Journal of Crop Protection,1998,40(1):16-22.

[3]徐永华,何志鸿,马淑梅.大豆疫霉病早熟抗源[J].大豆通报,1999(3):23-25.(Xu Y H,He Z H,Ma S M.Early-maturing soybean varieties with resistance to phytophthora megasperma[J].Soybean Bulletin,1999(3):23-25.)

[4]Wrather J A,Stienstra W C,Koenning S R.Soybean disease loss estimates for the United States from 1996 to 1998[J].Canadian Journal of Plant Pathology,2001,23(2):122-131.

[5]Wrather J A,Koenning S R,Anderson T R.Effect of diseases on soybean yields in the United States and Ontario(1999-2002)[J].Online.Plant Health Progress,2003,doi:10.1094/PHP-2003-0325-01-RV.

[6]李宝英,马淑梅,丁俊杰.大豆疫霉的发生危害及影响其发生因素的探讨[J].植物保护,1999,25(5):8-11.(Li B Y,Ma S M,Ding J J.Investigations of soybean phytophthoraroot rot disease and its determinant factors[J].Plant Protection,1999,25(5):8-11.)

[7]朱振东,霍云龙,王晓鸣,等.一个抗大豆疫霉根腐病新基因的分子鉴定[J].作物学报,2007,33(1):154-157.(Zhu Z D,Huo Y L,Wang X M,et al.Molecular identification of a novel?phytophthoraresistance gene in soybean[J].Acta Agronomica Sinica,2007,33(1):154-157.)

[8]Schmitthenner.A problems and progress in control of phytophthoraroot rot of soybean[J].Plant Disease,1985,69:362-368.

[9]韩英鹏,李文滨,Terry R A,等.耐大豆疫霉根腐病QTL定位的研究[J].大豆科学,2006,25(1):23-27.(Han Y P,Li W B,AndersonT R,et al.Mapping quantitative trait loci influencing tolerance to phytophthora?root rot in soybean[J].Soybean Science,2006,25(1):23-27.)

[10]Sun X,Liu D,Zhang X,et al.SLAF-seq:an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing[J].Plos One,2013,doi:10.1371/journal.pone.0058700.

[11]Bradbury P J,Zhang Z,Kroon D E,et al.TASSEL:Software for association mapping of complex traits in diverse samples[J].Bioinformatics,2007,23:2633-2635.

[12]Leitz R A,Hartman G L,Pedersen W L,et al.Races of phytophthora sojaeon soybean in Illinois[J].Plant Disease,2000,84(4):487.

[13]Li X P,Han Y P,Teng W L,et al.Pyramided QTL underlying tolerance to phytophthoraroot rot in mega-environments from soybean cultivars ‘Conrad’and ‘Hefeng 25’[J].Theoretical and Applied Genetics,2010,121:651-658.

[14]李修平,韩英鹏,丁俊杰,等.与耐大豆疫霉根腐病相关的QTL分析[J].大豆科学,2008,27(4):572-575.(Li X P,Han Y P,Ding J J,et al.Mapping quantitative trait loci underlining tolerance to phytophthoraroot rot in soybean[J].Soybean Science,2008,27(4):572-575.)


相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]任海龙,马启彬,杨存义,等.华南地区大豆育种材料抗疫霉根腐病鉴定[J].大豆科学,2012,31(03):453.[doi:10.3969/j.issn.1000-9841.2012.03.024]
　REN Hai-long,MA Qi-bin,YANG Cun-yi,et al.Screening Soybean Germplasms Resistant to Phytophthora sojae in South China[J].Soybean Science,2012,31(04):453.[doi:10.3969/j.issn.1000-9841.2012.03.024]
[12]杨晓贺,张 瑜,顾 鑫,等.大豆疫霉根腐病的综合防治[J].大豆科学,2014,33(04):554.[doi:10.11861/j.issn.1000-9841.2014.04.0554]
　YANG Xiao-he,ZHANG Yu,GU Xin,et al.Integrated Control of Soybean Phytophthora ?Root Rot[J].Soybean Science,2014,33(04):554.[doi:10.11861/j.issn.1000-9841.2014.04.0554]
[13]申宏波,胡志凤,丁俊杰,等.Harpins诱导苯丙氨酸解氨酶(PAL)活性防治大豆疫霉根腐病研究[J].大豆科学,2011,30(03):526.[doi:10.11861/j.issn.1000-9841.2011.03.0526]
　SHEN Hong-bo,HU Zhi-feng,DING Jun-jie,et al.Harpins Protein Alleviate Phytophthora megasperma by Adjusting PAL Enzyme Activity in Soybean[J].Soybean Science,2011,30(04):526.[doi:10.11861/j.issn.1000-9841.2011.03.0526]
[14]申宏波,丁俊杰,于永梅,等.2009年黑龙江省大豆新品系抗疫霉根腐病鉴定与评价[J].大豆科学,2010,29(06):1087.[doi:10.11861/j.issn.1000-9841.2010.06.1087]
　SHEN Hong-bo,DING Jun-jie,YU Yong-mei,et al.Identification and Evaluation on Soybean Lines Resistant to Phytophthora Root Rot from Heilongjiang Province in 2009[J].Soybean Science,2010,29(04):1087.[doi:10.11861/j.issn.1000-9841.2010.06.1087]
[15]李修平,韩英鹏,丁俊杰,等.与耐大豆疫霉根腐病相关的QTL分析[J].大豆科学,2008,27(04):572.[doi:10.11861/j.issn.1000-9841.2008.04.0572]
　LI Xiu-ping,HAN Ying-peng,DING Jun-jie,et al.Mapping Quantitative Trait Loci Underlining Tolerance to Phytophthora Root Rot in Soybean[J].Soybean Science,2008,27(04):572.[doi:10.11861/j.issn.1000-9841.2008.04.0572]
[16]陈丽霞,李英慧,郑服丛,等.油菜素内酯（BR）对大豆疫霉根腐病抗性的影响[J].大豆科学,2007,26(05):713.[doi:10.3969/j.issn.1000-9841.2007.05.015]
　CHEN Li-xia,LI Ying-hui,ZHENG Fu-cong,et al.EFFECT OF BRASSINOSTEROIDS ON SOYBEAN RESISTANCE TO PHYTOPHTHORA SOJAE[J].Soybean Science,2007,26(04):713.[doi:10.3969/j.issn.1000-9841.2007.05.015]
[17]刘世名,李魏,戴良英.大豆疫霉根腐病抗性研究进展[J].大豆科学,2016,35(02):320.[doi:10.11861/j.issn.1000-9841.2016.02.0320]
　LIU Shi-ming,LI Wei,DAI Liang-ying.Progresses in Research on the Resistance of Soybean to Phytophythora Root Rot Caused by Phytophthora sojae[J].Soybean Science,2016,35(04):320.[doi:10.11861/j.issn.1000-9841.2016.02.0320]
[18]韩英鹏 　 李文滨 　 Kangfu Yu 　 Terry R. Anderson Vaino Poysa 　 文景芝 　 高继国.耐大豆疫霉根腐病 QTL 定位的研究*[J].大豆科学,2006,25(01):23.[doi:10.11861/j.issn.1000-9841.2006.01.0023]
　Han Yingpeng Li Wenbin Kangfu Yu Terry R. Anderson Vaino Poysa Wen Jingzhi Gao Jiguo.MAPPING QUANTITATIVE TRAIT LOCI INFLUENCING TOLERANCE TOPHYTOPHTHORA ROOTROT IN SOYBEAN[J].Soybean Science,2006,25(04):23.[doi:10.11861/j.issn.1000-9841.2006.01.0023]
[19]马淑梅　李宝英　丁俊杰.大豆疫霉根腐病抗病资源筛选及抗性遗传研究[J].大豆科学,2001,20(03):197.[doi:10.11861/j.issn.1000-9841.2001.03.0197]
　Ma Shumei Li Baoying Ding Zunjie.SELECTIONS OF SOYBEAN GERMPLASMSWITH RESISTANCE TOPEYTOPHTHORA ROOT ROT AND ITS USE IN RTEEDINGFOR RESISTANCE MA SHUMEI LI BAOYINGDING JUNJIE[J].Soybean Science,2001,20(04):197.[doi:10.11861/j.issn.1000-9841.2001.03.0197]
[20]马淑梅 丁俊杰 郑天琪 顾　 鑫.黑龙江省大豆疫霉根腐病生理小种鉴定结果＊[J].大豆科学,2005,24(04):260.[doi:10.11861/j.issn.1000-9841.2005.04.0260]
　Ma Shumei Ding Junjie Zheng Tianqi Gu Xin.THE IDENTIFICATION OF PHYSIOLOGICAL RACES OF PHY TOPH T HORA MEGASPERMA[J].Soybean Science,2005,24(04):260.[doi:10.11861/j.issn.1000-9841.2005.04.0260]

备注/Memo

基金项目：国家重点基础研究发展计划“973计划”前期项目（2012CB126311）；国家自然基金（31201227，31301339）；国家“十二五”科技支撑计划（2011BAD35B06-1）；现代农业产业技术体系项目（CARS-04-PS04）；中国博士后项目（20110491024）；黑龙江省博士后项目(LBH11220，LBH-TZ1210)；黑龙江省教育厅骨干教师资助项目（1252G014）；黑龙江省教育厅科学技术研究项目（12541049）；黑龙江省教育厅新世纪项目优秀人才项目（1253-NCET-005）；教育部博士点项目（20122325120012）；东北农业大学博士后启动金项目（2012RCB11）。

第一作者简介：赵雪(1981-)，女，博士， 助理研究员，主要从事分子辅助育种研究。E-mail：zhaoxue_s@163.com。
通讯作者：李文滨（1958-），男，博士，教授，主要从事大豆遗传育种研究。E-mail: wenbinli@neau.edu.cn。

更新日期/Last Update: 2014-09-11