[1]韩英鹏 　 李文滨 　 Kangfu Yu 　 Terry R. Anderson Vaino Poysa 　 文景芝 　 高继国.耐大豆疫霉根腐病 QTL 定位的研究*[J].大豆科学,2006,25(01):23-27.[doi:10.11861/j.issn.1000-9841.2006.01.0023]
　Han Yingpeng Li Wenbin Kangfu Yu Terry R. Anderson Vaino Poysa Wen Jingzhi Gao Jiguo.MAPPING QUANTITATIVE TRAIT LOCI INFLUENCING TOLERANCE TOPHYTOPHTHORA ROOTROT IN SOYBEAN[J].Soybean Science,2006,25(01):23-27.[doi:10.11861/j.issn.1000-9841.2006.01.0023]
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耐大豆疫霉根腐病 QTL 定位的研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年01期 页码: 23-27 栏目: 出版日期: 2006-02-25
Title:
MAPPING QUANTITATIVE TRAIT LOCI INFLUENCING TOLERANCE TOPHYTOPHTHORA ROOTROT IN SOYBEAN
作者:
韩英鹏 1 　 李文滨 1 　 Kangfu Yu 2 　 Terry R. Anderson 2Vaino Poysa 2 　 文景芝 3 　 高继国 4
(1. 东北农业大学大豆研究所,哈尔滨 150030 ;2. 加拿大农业部温室与加工研究所 加拿大;3. 东北农业大学农学院植保系,哈尔滨 150030 ;4. 东北农业大学生命科学院,哈尔滨 150030)
Author(s):
Han Yingpeng1　 Li Wenbin 1 　 Kangfu Yu 2 　 Terry R. Anderson 2 　 Vaino Poysa 2Wen Jingzhi3　 Gao Jiguo4
(1.Soybean Research Institute , Northeast Agricultural University , Harbin 150030;2. Agriculture andAgri-food Canada , Greenhouse and Processing Crop Research Center , Canada;3.College of Li f eSciences, Northeast Agricultural University , Harbin 150030;4.Department of Plant Protection ,Agricultural college , Northeast Agricultural University , Harbin 150030)
关键词:
大豆; 疫霉根腐病; QTL ; 耐病性
Keywords:
Soybean ; Phytophthora root rot ; QTLs; Tolerance
DOI:
10.11861/j.issn.1000-9841.2006.01.0023
文献标志码:
A
摘要:
利用1200 个 RAPD 随机引物和 341 对 SSR 引物对 Conrad ×OX760的重组自交系(RIL)F 2:6 群体的耐大豆疫霉根腐病基因进行 QTL 定位 。试验设两个地点、两个年限, 在 MLG D1b +W和 MLG M 连锁群上检测到 3 个 QTL ,即 QP - 1 (OPL18 - 800bp) 、QP - 2 (OPN03 - 600bp) 和QP - 3 (Satt536 和 Satt463)。每个 QTL 对病害损失率的贡献率从 13. 34%到 22. 31 %不等。QP- 1 和 QP - 2 经多重 QT L 分析(Mapmaker/ QT L1. 1)对两年 (2000 年和 2001 年)两点(Wood-slee 和 Werver)平均病害损失率的贡献率合计达 44. 5% , QP - 3 对 2001 年 Woodslee 试验点的病害损失率的贡献率为 15. 2%, QP - 1 对 2000 年 Woodslee 试验点的病害损失率的贡献率为21. 55%, 对 2000 年 Wever 试验点的病害损失率的贡献率 16. 71% , 及对两年两点平均病害损失率的贡献率为 22. 31%。在大多数生态环境下能稳定的、再次被检测出的 QTL , 可以作为育种工作中的重点选择指标 ,用以指导耐大豆疫霉根腐病品种的分子辅助选育。
Abstract:
　 T he purpose of this research is to identify quantitative trait loci (QT Ls) on the linkage groupswhich are associated with Soybean phytophthora root rot (PRR), in a population from a cross betweenConrad , a soybean cultivar tolerance to PRR in the field, and OX760, a breeding lines susceptible to phy-thora. DNA amplified via polymerase chain reaction using simple sequence repeat (SSR)marker and Ran-dom Amplified Polymorphic DNA (RAPD)marker. Based on the data of PRR at two locations , Woodslee(2000 and 2001 ) ,Weaver (2000) , and the two locations, two years average loss,four markers OPL18 -800bp, OPN03- 600bp;Satt536 , Satt463 , from two linkage groups , MLGD1b+W , MLGM ,were sig-nificantly associated with PRR . Each marker explained 13. 34%to 22. 31 %of phenotypic variance. T hreeputative QTLs :Q P - 1(OPL18- 800bp)Q P - 2 (OPN03 - 600bp) , Q P - 3 (Satt536 and Satt463 )cor-responding to these four markers , were designed. Multiple QT L analysis(Mapmaker / QTL1. 1)showedQP1 and Q P - 2 jointly explained 44. 5%of the phenotypic variance for the two locations ,two years aver-age loss . Q P - 3 explained 15. 2% of the phenotypic variance in Woodslee for 2001. QP - 1 explained21. 55%of that in Woodslee for 2000, 16. 71%of that in Wever for 2000, 22. 31%of that for the two loca-tions, two years average loss. Few QTLs were detected to be stable across most environments, which wasuseful for marker assisted-breeding.

参考文献/References:

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备注/Memo

项目来源:本研究是 863 项目《大豆优质多抗基因分子标标辅助育种及聚合育种技术》的部分内容(项目编号:2003AA207060 - 4)。

?作者简介:韩英鹏(1978 -), 男, 硕士研究生, 研究方向大豆生物技术。　
?通讯作者:李文滨, E - mail:wenbinli@yahoo. com, 高继国

更新日期/Last Update: 2016-12-12