[1]刘 硕,罗 玲,刘章雄,等.大豆蛋白质含量QTL的“整合”及Overview分析[J].大豆科学,2011,30(01):1-7,14.[doi:10.11861/j.issn.1000-9841.2011.01.0001]
　LIU Shuo,LUO Ling,LIU Zhang-xiong,et al.Integration of QTLs Related to Soybean Protein Content and “Qualification” of Them by Overview Method[J].Soybean Science,2011,30(01):1-7,14.[doi:10.11861/j.issn.1000-9841.2011.01.0001]
点击复制

大豆蛋白质含量QTL的“整合”及Overview分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年01期 页码: 1-7,14 栏目: 出版日期: 2011-02-25
Title:
Integration of QTLs Related to Soybean Protein Content and “Qualification” of Them by Overview Method
文章编号:
1000-9841（2011）01-0001-07
作者:
刘 硕1; 2; 罗 玲1; 刘章雄1; 关荣霞1; 邱丽娟2
1.南昌大学 生命科学学院，江西 南昌330031；
2.中国农业科学院 作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京 100081
Author(s):
LIU Shuo1; 2;  LUO Ling1;  LIU Zhang-xiong1;  GUAN Rong-xia1;  QIU Li-juan2
1.College of Life Sciences, Nanchang University, Nanchang 330031, Jiangxi;
2.National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Germplasm & Biotechnology (MOA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
大豆; 蛋白质; 物理整合; 元分析; Overview; 优化
Keywords:
Key words:Soybean;  Protein;  Physical integration;  Meta-analysis;  Overview;  Qualification
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0001
文献标志码:
A
摘要:
在物理整合的基础上，选用元分析方法将已发表的与蛋白质相关的QTL整合到物理整合中重复次数较多的6个QTL区间处，并且选用基于统计学原理的Overview方法进行优化，得到连锁群上QTL的有效遗传位置，最终筛选出稳定性较强的标记Satt127，研究结果为标记辅助鉴定提供了理论依据。
Abstract:
Soybean protein is the main source of vegetable protein. Developing high protein content soybean is one of main aims in soybean breeding. Up to now, lots of QTLs have been revealed related to soybean protein content, but due to the different populations, markers and environments, the QTLs have not been integrated systematically. This study mainly referred to the physical integrations of QTL results and select meta-analysis to confirm 6 stable QTL regions. Moreover, overview method based on statistic thesis was also chosen for qualifications of QTLs and confirmations of valid QTLs positions. Finally, the most stable SSR marker Satt127 had been screened out, which could apply evidence for marker-assisted breeding.

参考文献/References:

[1]Apuya N R, Frazier B L, Keim P, et al. Retriction fragment length polymorphism as genetic marker in soybean[J]. Theoretical and Applied Genetics, 1988, 75：889-901.

[2]Song Q J, Marek L F, Shoemaker R C, et al. A new integrated genetic linkage map of the soybean[J]. Theoretical and Applied Genetics, 2004, 109：122-128.

[3]Concibido V C, Diers B W, Arelli P R. A decade of QTL mapping for cyst nematode resistance in soybean[J]. Crop Science, 2004, 44：1121-1131.

[4]Glass G V. Primary, secondary, and meta-analysis of research[J]. Educational Researcher, 1976, 5：3-8.

[5]Guo B, Sleper D A, Lu P, et al. QTLs associated with resistance to soybean cyst nematode in soybean：meta-analysis of QTL location[J]. Crop Science, 2006, 46：595-602.

[6]吴琼，齐照明，刘春燕，等．基于元分析的大豆生育期QTL的整合[J]．作物学报，2009，35（8）：1418-1424．（Wu Q, Qi Z M, Liu C Y, et al. An integrated QTL map of growth stage in soybean(Glycine maxL. Merr.)：Constructed through Meta-Analysis[J]. Acta Agronomica Sinica, 2009, 35(8)：1418-1424．)

[7]齐照明，孙亚男，陈立君，等．基于Meta分析的大豆百粒重的QTLs定位[J]．中国农业科学，2009，42（11）：3795-3803．(Qi Z M, Sun Y N, Chen L J, et al. Meta-analysis of 100-seed weight QTLs in soybean[J]. Scientia Agricultura Sinica, 2009, 42(11)：3795-3803．)

[8]宋万坤，王晶，朱命喜，等．大豆脂肪酸组分相关QTL元分析[J]．大豆科学，2009，28（5）：774-780．(Song W K, Wang J, Zhu M X, et al. Meta-analysis of fatty acid QTLs in soybean[J]. Soybean Science, 2009, 28(5)：774-780．)

[9]Wang J L, Liu C Y, Wang J, et al. An integrated QTL map of fungal disease resistance in soybean(Glycine maxL. Merr)：A method of meta-analysis for mining R genes[J]. Agricultural Science in China, 2010, 9(2)：223-232.

[10]Chardon F, Virlon B, Moreau L, et al. Genetic architecture of flowing time in maize as inferred from quantitative trait loci meta-analysis and sunteny conservation with the rice genome[J]. Genetic, 2004, 168：2169-2185.

[11]王毅．玉米本地化生物信息库的构建和QTL的整合、比较和元分析[D]．武汉：华中农业大学，2006．(Wang Y. The construction of local maize bioinformatics database and integration, comparison and meta-analysis of QTL[D]. Wuhan：Huazhong Agricultural University, 2006．)

[12]史利玉．玉米抗粗缩病及灰斑病基因的初步定位[D]．成都：四川农业大学，2007．(Shi L Y. The general location of gene of MRDV and GLS in maize[D]. Chengdu：Sichuan Agricultural University, 2007．)

[13]Brummer E C, Graef G L, Orf J, et al. Mapping QTL for seed protein and oil content in eight soybean populations[J]. Crop Science, 1997, 37：370-378.

[14]Chapman A, Pantalone V R, Ustun A, et al. Quantitative trait loci for agronomic and seed quality traits in F2?and F4:6soybean population[J]. Euphytica, 2003, 129：387-393.

[15]Chen Q S, Zhang Z C, Liu C Y, et al. QTL analysis of major agronomic traits in soybean[J]. Agricultural Sciences in China, 2007, 6(4)：399-405.

[16]Chung J, Babka H L, Graef G L, et al. The seed protein, oil, and yield QTL on soybean linkage group I[J]. Crop Science, 2003, 43：1053-1067.

[17]Csanadi G, Vollmann J, Stift G, et al. Seed quality QTLs identified in a molecular map of early maturing soybean[J]. Theoretical and Applied Genetics, 2001, 103：912-919.

[18]Diers B W, Keim P, Fehr W R, et al. RFLP analysis of soybean seed protein and oil content[J]. Theoretical and Applied Genetics, 1992, 83：608-612.

[19]Fasoula V A, Harris D K, Boerma H R. Validation and designation of quantitative trait loci for seed protein, seed oil, and seed weight from two soybean populations[J]. Crop Science, 2004, 44：1218-1225.

〖HJ〗[20]Gai J Y, Wang Y J, Wu X L, et al. A comparative study on segregation analysis and QTL mapping of quantitative traits in plants-with a case in soybean[J]. Frontier of Agriculture in China, 2007, 1(1)：1-7.

[21]Hyten D L, Pantalone V R, Sams C E, et al. Seed quality QTL in a prominent soybean population[J]. Theoretical and Applied Genetics, 2004, 109：552-561.

[22]Jun T H, Van K J, Kim M Y, et al. Association analysis using SSR markers to find QTL for seed protein content in soybean[J]. Euphytica, 2008, 162：179-191.

[23]Kabelka E A, Diers B W, Fehr W R, et al. Putative alleles for increased yield from soybean plant introductions[J]. Crop Science, 2004, 44：784-791.

[24]Lee S H, Bailey M A, Mian M A R, et al. RFLP loci associated with soybean seed protein and oil content across populations and locations[J]. Theoretical and Applied Genetics, 1996, 93：649-657.

[25]Li W B, Sun D S, Du Y P, et al. Quantitative trait loci underlying the development of seed composition in soybean(Glycine maxL. Merr.)[J]. Genome, 2007, 50：1067-1077.

[26]Mansur L M, Orf J H, Chase K, et al. Genetic mapping of agronomic traits using recombinant inbred lines of soybean[J]. Crop Science, 1996, 36：1327-1336.

[27]Orf J H, Chase K, Jarvik T, et al. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations[J]. Crop Science, 1999, 39：1642-1651.

[28]Panthee D R, Pantalone V R, West D R, et al. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean[J]. Crop Science, 2005, 45：2015-2022.

[29]Palomeque L, Liu L J, Li W B, et al. QTL in mega-environments： II. Agronomic trait QTL co-localized with seed yield QTL detected in a population derived from a cross of high-yielding adapted×high-yielding exotic soybean lines[J]. Theoretical and Applied Genetics, 2009, 119：429-436.

[30]Qiu B X, Arelli P R, Sleper D A. RFLP markers associated with soybean cyst nematode resistence and seed composition in a ‘Peking’בEssex’ population[J]. Theoretical and Applied Genetics, 1999, 98：356-364.

[25]Li W B, Sun D S, Du Y P, et al. Quantitative trait loci underlying the development of seed composition in soybean(Glycine maxL. Merr.)[J]. Genome, 2007, 50：1067-1077.

[26]Mansur L M, Orf J H, Chase K, et al. Genetic mapping of agronomic traits using recombinant inbred lines of soybean[J]. Crop Science, 1996, 36：1327-1336.

[27]Orf J H, Chase K, Jarvik T, et al. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations[J]. Crop Science, 1999, 39：1642-1651.

[28]Panthee D R, Pantalone V R, West D R, et al. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean[J]. Crop Science, 2005, 45：2015-2022.

[29]Palomeque L, Liu L J, Li W B, et al. QTL in megaenvironments： II. Agronomic trait QTL colocalized with seed yield QTL detected in a population derived from a cross of high?yielding adapted×highyielding exotic soybean lines[J]. Theoretical and Applied Genetics, 2009, 119：429-436.

[30]Qiu B X, Arelli P R, Sleper D A. RFLP markers associated with soybean cyst nematode resistence and seed composition in a ‘Peking’בEssex’ population[J]. Theoretical and Applied Genetics, 1999, 98：356-364.


相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]任红玉,周丽华,朱晓鑫,等.在UV-B辐射增强条件下稀土镧对大豆品质的影响[J].大豆科学,2013,32(03):345.[doi:10.11861/j.issn.1000-9841.2013.03.0345]
　REN Hong-yu,ZHOU Li-hua,ZHU Xiao-xin,et al.Effect of La on the Soybean Quality under Elevated Ultraviolet-B Radiaion[J].Soybean Science,2013,32(01):345.[doi:10.11861/j.issn.1000-9841.2013.03.0345]
[12]曹永强,宋书宏,董丽杰.大豆蛋白质和油分含量遗传研究进展[J].大豆科学,2012,31(02):316.[doi:10.3969/j.issn.1000-9841.2012.02.033]
　CAO Yong-qiang,SONG Shu-hong,DONG Li-jie.Research Progress on Heredity of Protein and Oil Content in Soybean[J].Soybean Science,2012,31(01):316.[doi:10.3969/j.issn.1000-9841.2012.02.033]
[13]葛振宇,刘晓冰,刘宝辉,等.大豆种子蛋白质和油份性状的QTL定位[J].大豆科学,2011,30(06):901.[doi:10.11861/j.issn.1000-9841.2011.06.0901]
　GE Zhen-yu,LIU Xiao-bing,LIU Bao-hui,et al.QTL Mapping of Protein and Oil Content in Soybean[J].Soybean Science,2011,30(01):901.[doi:10.11861/j.issn.1000-9841.2011.06.0901]
[14]邱强,刘宪虎,张伟,等.吉林省不同大豆品种脂肪和蛋白质含量生态分析[J].大豆科学,2012,31(05):749.[doi:10.3969/j.issn.1000-9841.2012.05.012]
　QIU Qiang,LIU Xian-hu,ZHANG Wei,et al.Ecological Analysis on Oil and Protein Content of Soybeans in Jilin Province[J].Soybean Science,2012,31(01):749.[doi:10.3969/j.issn.1000-9841.2012.05.012]
[15]林长库,姚鑫淼,陈凤山,等.黑龙江省大豆区域试验品种品质现状分析[J].大豆科学,2009,28(06):1031.[doi:10.11861/j.issn.1000-9841.2009.06.1031]
　LIN Chang-ku,YAO Xin-miao,CHEN Feng-shan,et al.Grain Quality of Recent Soybean Varieties（Lines）in Heilongjiang Province[J].Soybean Science,2009,28(01):1031.[doi:10.11861/j.issn.1000-9841.2009.06.1031]
[16]任红玉,高振宇,潘小燕,等.稀土镧和铈对大豆品质的影响[J].大豆科学,2014,33(04):603.[doi:10.11861/j.issn.1000-9841.2014.04.0603]
　REN Hong-yu,GAO Zhen-yu,PAN Xiao-yan,et al.Effect of Lanthanum and Cerium on Soybean Quality[J].Soybean Science,2014,33(01):603.[doi:10.11861/j.issn.1000-9841.2014.04.0603]
[17]李文滨,郑宇宏,韩英鹏.大豆种质资源脂肪酸组分含量及品质性状的相关性分析[J].大豆科学,2008,27(05):740.[doi:10.11861/j.issn.1000-9841.2008.05.0740]
　LI Wen-bin,ZHENG Yu-hong,HAN Ying-peng.Analysis of Fatty Acid Composition and Other Quality Traits in Soybean Varieties Developed in Heilongjiang Province[J].Soybean Science,2008,27(01):740.[doi:10.11861/j.issn.1000-9841.2008.05.0740]
[18]闫龙,蒋春志,于向鸿,等.大豆粗蛋白、粗脂肪含量近红外检测模型建立及可靠性分析[J].大豆科学,2008,27(05):833.[doi:10.11861/j.issn.1000-9841.2008.05.0833]
　YAN Long,JIANG Chun-zhi,YU Xiang-hong,et al.Development and Reliability of Near Infrared Spectroscopy(NIS) Models of Protein and Oil Content in Soybean[J].Soybean Science,2008,27(01):833.[doi:10.11861/j.issn.1000-9841.2008.05.0833]
[19]闫春娟,韩晓增,王树起,等.钾对大豆干物质积累、产量及品质的影响[J].大豆科学,2008,27(01):113.[doi:10.11861/j.issn.1000-9841.2008.01.0113]
　YAN Chun-juan,HAN Xiao-zeng,WANG Shu-qi,et al.Effect of Potassium Fertilizer on Dry Matter Accumulation，Yield and Quality of Soybean[J].Soybean Science,2008,27(01):113.[doi:10.11861/j.issn.1000-9841.2008.01.0113]
[20]刘萌娟,翟亚萍,李鸣雷.陕西省大豆品种资源蛋白质和脂肪含量研究[J].大豆科学,2007,26(04):533.[doi:10.3969/j.issn.1000-9841.2007.04.016]
　LIU Meng-juan,ZHAI Ya-ping,LI Ming-lei.A STUDY ON PROTEIN AND FAT CONTENTS OF SHANXI SOYBEAN ACCESSIONS[J].Soybean Science,2007,26(01):533.[doi:10.3969/j.issn.1000-9841.2007.04.016]

备注/Memo

基金项目：国家重点基础研究发展计划资助项目（2009CB118403）；国家转基因专项资助项目（2008ZX08009-003）；国际科技合作计划项目(2008DFA330550)。

第一作者简介：刘硕（1986-），男，在读硕士，研究方向为大豆农艺性状QTL定位。E-mail：ls20023002ls@yahoo.com.cn。
通讯作者：邱丽娟（1963-），女，研究员，博士生导师，研究方向为大豆种质资源及资源创新。E-mail：qiu_lijuan@263.net。

更新日期/Last Update: 2014-09-11