ZHANG Jing-yong,SUN Huan,ZHAO Li-mei,et al.Classification of Male-sterile Lines with RN Sterile Cytoplasm and Their Restorers[J].Soybean Science,2010,29(04):559-564.[doi:10.11861/j.issn.1000-9841.2010.04.0559]
大豆RN不育胞质不育与恢复类型的研究
- Title:
- Classification of Male-sterile Lines with RN Sterile Cytoplasm and Their Restorers
- 文章编号:
- 1000-9841(2010)04-0559-06
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 阐述了以RN不育细胞质为基础选育的细胞质雄性不育系和恢复系的类型。在大量测交以及预备试验的基础上,选择具有代表性的9对不育系和相应的保持系以及10个恢复系,进行不育系与所有保持系之间,以及不育系与所有恢复系之间所有可能的杂交。对不育系与恢复系杂交的F1,依据花粉败育率分为3类以表示恢复度,A类:败育率0~10%,B类:败育率11%~60%,C类:败育率61%以上。再依据这3类的分布情况和恢复谱,对不育系和恢复系进行分类。结果表明:任何一个保持系均可保持所有不育系,保持不育性的遗传机制较为简单,受隐性单基因控制。不育系分为极易被恢复,易被恢复和不易被恢复3类。依据恢复谱和恢复度将恢复系分为:强恢复,恢复和弱恢复3类。极易被恢复的不育系和强恢复系是大豆杂交种选育中的珍贵育种材料。由于育性的恢复比较复杂,测交中最好同时用上述3类不同类型的不育系做测交母本。
- Abstract:
- The classification of male-sterile lines with RN sterile cytoplasm and their restorers was described in this paper. Representative genetic stocks including 9 pairs of male-sterile lines and their maintainers and 10 restorers were selected as experimental materials based on comprehensive test crosses and a preliminary experiment. All possible crosses were made between male-sterile lines and their maintainers, as well as their restorers. The F1s from crosses between male-sterile lines and their restorers could be divided into three categories of A (0~10%), B(11%~60%)and C (≥61%) to indicate the degree of restoration according to the percentage of sterile pollen grains. Taking the distribution of A, B and C and the restoration spectrum as basic consideration the male sterile lines and the restorers were further classified. The result showed that any maintainer could maintain the sterility of all male sterile lines. The genetics of sterility maintenance seems simple: single recessive gene control. Three types of male sterile lines of very easy to be restored, easy to be restored and difficult to be restored were identified. The restorers could also be divided into three types-strong restorers, normal restorers and weak restorers. The male sterile lines that are very easy to be restored and the strong restorers are very valuable in the use of heterosis breeding. It is suggested that all three types of male sterile lines should be included as female testers in test crosses since the sterility restoration is quite complicated.
参考文献/References:
[1]孙寰,赵丽梅,黄梅.大豆质-核互作不育系研究[J].科学通报,1993,38(16):1535-1536.( Sun H,Zhao L M, Huang M. Studies on cytoplasmic-nuclear male sterile soybean [J].Chinese Science Bulletin, 1993,38(16):1535-1536.) [2]孙寰,赵丽梅,黄梅.质核互作雄性不育大豆及生产大豆杂交种的方法:中国, ZL97 1 12173.7[P].2000.( Sun H,Zhao L M, Huang M.Cytoplasmic-genetic male sterile soybean and method for producing hybrid soybean:China ,ZL97 1 12173.7[P].2000.) [3]孙寰,赵丽梅,王曙明,等.大豆杂种优势研究进展[J].中国油料作物学报,2003,25(1): 92-96,100.(Sun H,Zhao L M,Wang S M,et al.Review of soybean heterosis utilization[J].Chinese Journal of Oil Crop Sciences,2003,25(1): 92-96,100.) [4]赵丽梅,孙寰,黄梅.大豆细胞质雄性不育系ZA的选育和初步研究[J]. 大豆科学, 1998, 17(3): 268-270. (Zhao L M,Sun H,Huang M.The development and preliminary study cytoplasmic male sterile soybean line ZA[J]. Soybean Science, 1998, 17(3): 268-270.) [5]丁德荣,盖钧镒,崔章林,等.大豆质核互作雄性不育系NJCMS1A及其保持系NJCMS1B的选育与验证[J].科学通报,1998,43(17):1901-1902.(Ding D R,Gai J Y,Cui Z L,et al. Development and verification of soybean cytoplasmic-nuclear male-sterile line NJCMS1A and NJCMS1B[J]. Chinese Science Bulletin, 1998,43(17):1901-1902.) [6]张磊,戴欧和.大豆质核互作不育系W931A的选育研究[J].中国农业科学,1997,30(6):90-91.( Zhang L,Dai O H,Selection and breeding of nucleo- cytoplasmic male sterile line W931A in Soybean[J].Scientia Agricultura Sinica, 1997,30(6):90-91.) [7]张磊,戴欧和,黄志平,等.大豆质核互作M型雄性不育系的选育及其育性表现[J]. 中国农业科学, 1999, 32(4): 34-38.(Zhang L,Dai O H,Huang Z P,et al.Selection of soybean male sterile line of nucleo-cytoplasmic interaction and its fertility[J]. Scientia Agricultura Sinica, 1999, 32(4): 34-38.) [8]许占友,李磊,邱丽娟,等. 大豆三系的选育及恢复基因的SSR初步定位研究[J]. 中国农业科学, 1999, 32(2): 32-38. (Xu Z Y,Li L,Qiu L J,et al. Selection of three lines and localization of the restorer genes in soybean using SSR markers[J]. Scientia Agricultura Sinica,1999, 32(2): 32-38.) [9]Bai Y N, Gai J Y. Development of a new cytoplasmic-nuclear male-sterile line of soybean and inheritance of its male-sterile restoration[J]. Plant Breeding, 2006, 125: 85-88. [10]Zhao T J,Gai J Y. Discovery of new male-sterile cytoplasm sources and development of a new cytoplasmic-nuclear male-sterile line NJCMS3A in soybean[J]. Eupytica, 2006, 152: 387-396. [11]白羊年,盖钧镒. 大豆质核互作雄性不育系NJCMS1A恢复源与保持源的鉴定[J]. 大豆科学, 2003, 22(3):161-165.( Bai Y N,Gai J Y.Identification of roestorers and maintainers of the cytoplasmic-nuclear male-sterile line NJCMS1A of soybean[J]. Soybean Science, 2003, 22(3):161-165.) [12]Sun H. A new approach of soybean improvement:hybrid soybean[C].Proceedings of World Soybean Research Conference Ⅷ, Plenary session, 2009. [13]赵丽梅,孙寰,黄梅,等.大豆结实率与花粉败育率之间的关系[J].大豆科学, 2004, 23(4):249-252.(Zhao L M,Sun H,Huang M,et al.The relationship between seed setting rate and pollen sterility rate for soybean[J]. Soybean Science, 2004, 23(4):249-252.) [14]孙寰,赵丽梅,王曙明,等. 大豆花粉育性分类标准的研究[J].大豆科学, 2006,25(4):339-343.(Sun H, Zhao L M,Wang S M,et al.Criterion on classification of pollen fertility in soybean[J]. Soybean Science, 2006,25(4):339-343.) [15]Kaul M L H.Male sterility in higher plant, in monographs on theoretical and applied genetics[M].Springer Verlag, Berlin, Germany,1988. [16]Gai J Y, Cui Z L, Ji D F,et al.A report on the nuclear cytoplasmic male sterility from a cross between two soybean cultivars[J]. Soybean Genetic Newsletters,1995,22:55-58.
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备注/Memo
基金项目:国家高技术研究发展计划资助项目(2009AA101106)。