[1]王丹,乔亚科,韩粉霞,等.河北东部沿海地区野生大豆SSR多样性分析[J].大豆科学,2010,29(04):555-559.[doi:10.11861/j.issn.1000-9841.2010.04.0555]
　WANG Dan,QIAO Ya-ke,HAN Fen-xia,et al.Genetic Diversity of Glycine soja in Eastern Coastal Area of Hebei Province[J].Soybean Science,2010,29(04):555-559.[doi:10.11861/j.issn.1000-9841.2010.04.0555]
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河北东部沿海地区野生大豆SSR多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年04期 页码: 555-559 栏目: 出版日期: 2010-08-25
Title:
Genetic Diversity of Glycine soja in Eastern Coastal Area of Hebei Province
文章编号:
1000-9841（2010）04-0555-04
作者:
王丹1; 乔亚科1; 韩粉霞2; 李桂英2; 蒲伟凤1; 代波1; 李桂兰1
1.河北科技师范学院 植物细胞工程实验室, 河北 昌黎 066600；
2.中国农业科学院 作物科学研究所，北京 100081
Author(s):
WANG Dan1;  QIAO Ya-ke1;  HAN Fen-xia2;  LI Gui-ying2;  PU Wei-feng1; DAI Bo1; LI Gui-lan1
1.Plant Cell Engineering Lab, Hebei Normal University of Science and Technology, Changli 066600，Hebei;
2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
冀东沿海; 野生大豆; SSR; 遗传多样性
Keywords:
Eastern area of Hebei Province;  Wild soybean;  SSR;  genetic diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0555
文献标志码:
A
摘要:
以采集于冀东沿海地区的370及黑龙江省的2份野生大豆为材料，利用27对SSR引物进行遗传多样性分析。27个SSR位点扩增出209个多态性带，平均每个位点等位基因数目为7.74个，SSR位点的遗传多样性指数分布范围, Simpson 指数为0.3998~0.8358， Shannon-weaver指数为0.7567~1.9879，冀东沿海地区野生大豆材料表现出丰富的遗传多样性。聚类分析结果中冀东靠近海岸线地区的材料与内陆材料之间存在着明显的遗传差异；同一居群的材料遗传距离很近，有些不同居群的材料由于生境相似遗传距离也较近；然而有些同一居群内的材料表现出了明显的遗传分化。
Abstract:
Three hundred and seventy accession of wild soybean, collected from the eastern area of Hebei Province and two accession from Heilongjiang Province were used as materials in this study. Twenty-seven pairs SSR primers were used to analyze the genetic diversity of these materials. Two hundred and nine polymorphic bands were amplified by 27 pairs SSR primers. Genetic diversity index showed that distribution of Simpson index was 0.3998~0.8358, and distribution of Shannon-weaver index was 0.7567~1.9879, which suggestted that wild soybean collected from the eastern area of Hebei Province had abundant genetic diversity. Clustering analysis showed that, the materials collected near the coastline are clearly separated from the materials collected inland region. The genetic distance was very close among the materials in one population and also the materials of different populations because of their similar habitat. However, sometime tere were genetics differentiations in a same population.

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备注/Memo

基金项目：国家自然科学基金资助项目（30940050)；河北省自然科学基金资助项目（C2009000868）；河北省科技支撑计划资助项目（09220103D，06547005D-3）;转基因生物新品种培育科技重大专项资助项目（2009ZX108004-00413）。

第一作者简介：王丹（1984-），女，在读硕士，研究方向为植物分子生物学。E-mail: wangdan7750158@163.com。
通讯作者：李桂兰，教授。E-mail:lgl63@126.com。

更新日期/Last Update: 2014-09-14