[1]陈立杰,陈井生,董健,等.放线菌次生代谢产物对不同来源大豆胞囊线虫J2毒性的研究[J].大豆科学,2008,27(04):637-640.[doi:10.11861/j.issn.1000-9841.2008.04.0637]
　CHEN Li-jie,CHEN Jing-sheng,DONG Jian,et al.Toxicity of Secondary Metabolites of Actinomycetes on Heterodera glycines J2[J].Soybean Science,2008,27(04):637-640.[doi:10.11861/j.issn.1000-9841.2008.04.0637]
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放线菌次生代谢产物对不同来源大豆胞囊线虫J2毒性的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 637-640 栏目: 出版日期: 2008-08-25
Title:
Toxicity of Secondary Metabolites of Actinomycetes on Heterodera glycines J2
文章编号:
1000-9841(2008)04-06374-04
作者:
陈立杰; 陈井生; 董健; 朱晓峰; 王媛媛; 段玉玺
沈阳农业大学植物保护学院植物线虫学研究室，辽宁 沈阳
Author(s):
CHEN Li-jie; CHEN Jing-sheng; DONG Jian; ZHU Xiao-feng; WANG Yuan-yuan; DUAN Yu-xi
Plant Nematology Laboratory,Shenyang Agricultural University,Shenyang 110161,Liaoning，China
关键词:
放线菌; 代谢产物; 大豆胞囊线虫J2; 毒性
Keywords:
Actinomycetes; Metabolites; Heterodera glycines J2; Toxicity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0637
文献标志码:
A
摘要:
抗生素是控制植物病害的重要手段之一,而放线菌是抗生素的主要产生菌。针对已筛选到的5株放线菌C25-3、H-4、H-2、C44和C49，利用室内培养法对其发酵液抑制田间大豆胞囊线虫J2混合群体的活性作用进行了比较研究。结果表明：发酵液4×稀释浓度下，处理24 h后，C25-3对大豆胞囊线虫J2的毒性最高，其校正死亡率达到95%左右；其他4株菌的次生代谢产物也具有一定的毒杀作用，对J2的校正死亡率均达到60%以上。5株放线菌菌株中， C25-3，H-2和C44对大豆胞囊线虫J2的毒性不因线虫的致病性和活性差异而改变，而C49和H-4表现出因线虫的致病性和活性差异而改变的特性。
Abstract:
Plant parasitic nematode could be controlled by antibotics effectively.It is well-known that actinomycetes should be able to produce antibiotics.At present，we had screened 5 strain actinomycetes，and tested their activity on soybean cyst nematode J2 by means of separate technique and intro-culture fermentation.Effects of secondary metabolites of actinomycetes on Heterodera glycines-J2 showed that: fermentation filtrates of strain C25-3 manifested the highest level of toxicity on J2，and the other strains also had been effective.The corrected mortality rate of J2 reached 95% by strain C25-3 at 4 diluted solution，and the other strains also reached beyond 60%.Activity of the strains C25-3，H-2 and C44 had never varied with different phenotype cyst nematodes J2 which came from Heilongjiang and Liaoning province，on the contrary，the strains C49 and H-4 displayed different level of toxicity.The phenotype cyst nematodes of Heilongjiang included race 3，race 4 and race 14，and that of Liaoning only included race 3.So we found some actinomycetes could produce broad spectrum antibiotics for different phenotype cyst nematodes and others were opposite in this research.

参考文献/References:

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相似文献/References:

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　CHEN Jing-sheng,CHEN Li-jie,LIU Da-wei,et al.Indentification of the Strain Snea49 and Evaluation for Nematicidal Activity Against Soybean Cyst Nematode[J].Soybean Science,2010,29(04):663.[doi:10.11861/j.issn.1000-9841.2010.04.0663]
[2]陈井生,周园园,朱峰,等.大豆胞囊线虫生防菌Snea49代谢物对不同种类线虫的毒力[J].大豆科学,2015,34(03):527.[doi:10.11861/j.issn.1000-9841.2015.03.0527]
　CHEN Jing-sheng,ZHOU Yuan-yuan,ZHU Feng,et al.Nematicidal Activity Analysis of Streptomyces Snea49 Metabolic Product[J].Soybean Science,2015,34(04):527.[doi:10.11861/j.issn.1000-9841.2015.03.0527]
[3]赵丽珍,刘振钦.香菇代谢产物对大豆增产作用的研究[J].大豆科学,1996,15(01):80.[doi:10.11861/j.issn.1000-9841.1996.01.0080]
　[J].Soybean Science,1996,15(04):80.[doi:10.11861/j.issn.1000-9841.1996.01.0080]

备注/Memo

基金项目：霍英东青年教师基金资助项目（101033）；国家自然科学基金资助项目（30671399）；辽宁省优秀人才支持计划资助项目。

作者简介：陈立杰(1971-),女，副教授,博士，现从事植物病理学和线虫学的教学和科研工作。E-mail:chenlj@syau.edu.cn。

更新日期/Last Update: 2014-10-07