[1]张军政,杨谦,魏丹,等.东北黑土区核盘菌对大豆寄主的破坏性研究[J].大豆科学,2008,27(04):633-636.[doi:10.11861/j.issn.1000-9841.2008.04.0633]
　ZHANG Jun-zheng,YANG Qian,WEI Dan,et al.Research on Destructive of Sclerotinia Sclerotiorum to Host Soybean in Black Soil Area of Northeast China[J].Soybean Science,2008,27(04):633-636.[doi:10.11861/j.issn.1000-9841.2008.04.0633]
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东北黑土区核盘菌对大豆寄主的破坏性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 633-636 栏目: 出版日期: 2008-08-25
Title:
Research on Destructive of Sclerotinia Sclerotiorum to Host Soybean in Black Soil Area of Northeast China
文章编号:
1000-9841(2008)04-0633-04
作者:
张军政1; 杨谦1; 魏丹1; 2; 杨雷1; 张喜林2; 陈雪莉2
1哈尔滨工业大学，黑龙江 哈尔滨 150001?
?2黑龙江省农业科学院土壤肥料研究所，黑龙江 哈尔滨 150086
Author(s):
ZHANG Jun-zheng1; YANG Qian1; WEI Dan1; 2; YANG Lei1; ZHANG Xi-lin2; CHEN Xue-li2
1Harbin Institute of Technology，Harbin 150001；
2Institute of Soil Fertilizer and Environmental Resources，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 核盘菌; 破坏性
Keywords:
Soybean; Sclerotinia sclerotiorum; Destructive
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0633
文献标志码:
A
摘要:
大豆菌核病已成为我国大豆产区的主要病害之一，而且对大豆产量和品质性状的影响愈来愈大。为揭示不同大豆品种受到核盘菌侵染后植株形态，籽粒产量和品质的变化，收集黑土区不同地点核盘菌菌核进行分离，观察病原菌对大豆寄主形态学危害性，分析其对寄主品质性状的影响。结果表明：不同地区菌核分离物其形态特征是不同的；病原菌首先侵染大豆子叶产生白色菌丝，进而侵染大豆叶片、茎秆、荚，最终在茎秆内、荚中形成菌核。大豆寄主受到核盘菌病原侵染后，百粒重下降9.9%～20.8%，脂肪、蛋白质含量分别下降0.95%～1.81%和0.17%～1.12%，氨基酸总量下降0.59%～4.98%。3个大豆品种中17种氨基酸含量变化是垦鉴4号氨基酸均有下降趋势；哈97-124品系只有一种氨基酸含量没有下降（组氨酸）；黑农35则有5种氨基酸（组氨酸、赖氨酸、丙氨酸、甘氨酸、丝氨酸）含量没有下降。
Abstract:
Sclerotia of Sclerotinia sclerotiorum is one of chief diseases in soybean planting area in China，which could damage yield and quality of soybean seriously.To observe changes of plant agronomic traits of different soybean variety infected by sclerotia from Sclerotinia sclerotiorum and inspect content of protein，oil and amino acids.Sclerotia of ?Sclerotinia sclerotiorum were collected and isolated from different area of black soil，in order to observe the harmfulness of pathogens to host soybean morphology and analysis the effect to host quality.The result indicated that morphology of sclerotia isolated from different area was different.Pathogens infected soybean cotyledon first and produced white mycelium，then infected soybean leaves，stems，pods，finally formatted sclerotia in stems andpods.Host soybean infected by sclerotia pathogen，100-seed weight decreased 9.9%-20.8%，content of fat and protein decreased 0.95%-1.81% and 0.17%-1.21% respectively，and total content of amino acids decreased 0.59%-4.89%.For the17 species of amino acids in three soybean varieties，all amino acids in Kenjian 4 were decreased ，in Ha 97-124 only one amino acid (His) content hasn’t decreased and content of five amino acids (His，Lys，Ala，Gly and Ser) in Heinong 35 have not decreased.

参考文献/References:

[1]Purdy L H.Sclerotinia sclerotiorum:history，diseases and symptomatology，host range，geographic distribution，and impact[J].Phytopathology，1979，69：875-880.

[2]杨谦.核盘菌[M].哈尔滨：黑龙江科学技术出版社，1994：22-24.(Yang Q.Sclerotinia sclerotiorum(Lib.) de Bary[M].Harbin：Heilongjiang Science and Technology Press,1994：22-24.)
[3]姚浩然.黑龙江省大豆菌核病发生与防治[J].黑龙江农业科学，1989(5)：3.（Yao H R.Occurrence and control of ?Sclerotina sclerotirum of soybean in Heilongjiang Province[J].Heilongjiang Agricultural Science，1989(5):3.）
[4] Wegulo S N，Yang X B，Martinson C A.Soybean cultivar responses to Sclerotinia sclerotiorum in field and controlled environment studies[J].Plant Disease，1998，82：1264-1270.
[5] Pennypacker B W，Risius M L.Environmental sensitivity of soybean cultivar response to Sclerotinia sclerotiorum[J].Phytopathology，1999，89：618-622.
[6] Ziman L，Jedryczka M，Srobarova A.Relationship between morphological and biochemical characteristics of Sclerotinia sclerotiorum isolates and their aggressivity[J].Zeitschrift fur Pflanzenkrankheiten und Pflanzenaschutz，1998，105(3)：283-288.
[7]王汉中，刘贵华，郑元本，等.抗菌核病双低油菜新品种中双9号选育及其重要防御酶活性变化规律的研究[J].中国农业科学，2004,37(1):23-28.(Wang H Z，Liu G H，Zheng Y B，et al.Breeding of a Brassica napus cultivar Zhongshuang No.9 with high-resistance to Sclerotinia sclerotiorum and dynamics of its important defense enzyme activity[J].Scientia Agricultura Sinica，2004,37(1):23-28.)
[8] Kim H S，Sneller C H，Diers B W.Evaluation of soybean cultivars for resistance to sclerotinia stem rot in field environments[J].Crop Science，1999，39：64-68.
[9]Nelson B D，Helms T C，Olson M A.Comparison of laboratory and field evaluations of resistance in soybean to Sclerotinia sclerotiorum[J].Plant Disease，1991，75：662-665.
[10]刘胜毅，周必文，张建坤，等.甘蓝型油菜抗菌核病扩展的遗传分析[J].中国油料作物学报，2003，25(1):54-58.（Liu S Y，Zhou B W，Zhang J K，et al.Inheritance of resistance to Sclerotinia disease spread in oilseed rape[J].Chinese Journal of Oil Crop Sciences，2003，25(1):54-58.）
[11]Kim H S，Hartman G L，Manandhar J B，et al.Reaction of soybean cultivars to sclerotinia stem rot in field，greenhouse，and laboratory evaluations[J].Crop Science，2000，40：665-669.
[12]Maxwell D P，Lumsden R D.Oxalic acid production by Sclerotinia sclerotiorum in infected bean and in culture[J].Phytopathology，1970，60：1395-1398.

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备注/Memo

基金项目：黑龙江省杰出青年基金资助项目（JC200622）；黑龙江省自然科学基金资助项目（C2004-17）；国家科技支撑计划资助项目（2006BAD25B05）。

作者简介：张军政（1963-），男， 在读博士，讲师， 主要研究方向为环境工程。E-mail:zhangjunzheng_2008@126.com。

更新日期/Last Update: 2014-10-07