[1]孙欣,刘丽君,薛永国,等.接种疫霉根腐菌对大豆苯丙氨酸解氨酶活性的影响[J].大豆科学,2008,27(04):641-644.[doi:10.11861/j.issn.1000-9841.2008.04.0641]
　SUN Xin,LIU Li-jun,XUE Yong-guo,et al.Changes of L-phenylalanin ammo-nialyase Activity in Soybean inoculated with Phytophthora sojae[J].Soybean Science,2008,27(04):641-644.[doi:10.11861/j.issn.1000-9841.2008.04.0641]
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接种疫霉根腐菌对大豆苯丙氨酸解氨酶活性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 641-644 栏目: 出版日期: 2008-08-25
Title:
Changes of L-phenylalanin ammo-nialyase Activity in Soybean inoculated with Phytophthora sojae
文章编号:
1000-9841(2008)04-0641-04
作者:
孙欣; 刘丽君; 薛永国; 杨喆
黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨150086
Author(s):
SUN Xin; LIU Li-jun; XUE Yong-guo; YANG Zhe
Soybean Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
关键词:
大豆疫霉根腐病; 苯丙氨酸解氨酶; 酶活性
Keywords:
Phytophthora Root Rot; L-phenylalanin ammo-nialyase; Enzymatic activity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0641
文献标志码:
A
摘要:
大豆疫霉根腐病是一种危害严重的疫霉病害，对于抗病性的生理生化机制研究很少。苯丙氨酸解氨酶是联系初级代谢和次级代谢的关键酶，对于植物抗病性具有重要意义。为探讨苯丙氨酸解氨酶活性变化与大豆对疫霉根腐病抗性的关系，对不同抗、感病大豆品种接种疫霉根腐菌1号生理小种前及接种24、48、72、96 h后测定叶片中苯丙氨酸解氨酶活性的变化特点进行分析，探讨苯丙氨酸解氨酶在抗大豆疫霉根腐病中所起的作用。结果表明：接种大豆疫霉菌1号生理小种后，抗、感病品种叶肉细胞中苯丙氨酸解氨酶活性都呈现出规律性变化趋势，但在抗、感病大豆品种中峰值变化幅度不同，表明大豆对疫霉根腐病的抗性与苯丙氨酸解氨酶活性有关。
Abstract:
Soybean Phytophthora root rot is a serious Phytophthora disease.L-phenylalanin ammo-nialyase（PAL）as a key metabolic enzyme that linked the primary and secondary metabolism，is important to disease resistance of plant.To discuss the relationship between PAL activity and Phytophthora root rot resistance in soybean.Soybean cultivars resistant or sensitive to Phytophthora root rot were inoculated with Phytophthora sojae-race 1,and the PAL activity before inoculation and 24,48,72 and 96 h after inoculation was determined.The result showed that the PAL enzymatic activity changed regularly after being inoculated by Phytophthora sojae-race 1,while the changing ranges were different in resistant and sensitive soybean cultivars.Therefore,there is some relationship between the PAL activity and the resistance of soybean to Phytophthora root rot.

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备注/Memo

基金项目：国家高技术研究发展计划“863”计划资助项目（2006AA10Z1F9）。

作者简介：孙欣(1979-)，女,硕士， 研究方向为植物学。

更新日期/Last Update: 2014-10-07