[1]张立军,宋广周,白霜,等.H2O2对不同大豆品种低温萌发及主要抗氧化酶活性的影响[J].大豆科学,2008,27(01):97-100，105.[doi:10.11861/j.issn.1000-9841.2008.01.0097]
　ZHANG Li-jun,SONG Guang-zhou,BAI Shuang,et al.Effect of Exogenous H2O2on Germination Rate and Activities of Main Antioxidase in Soybean (Glycine maxL.) Seedlings under Low Temperature[J].Soybean Science,2008,27(01):97-100，105.[doi:10.11861/j.issn.1000-9841.2008.01.0097]
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H2O2对不同大豆品种低温萌发及主要抗氧化酶活性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 97-100，105 栏目: 出版日期: 2008-02-25
Title:
Effect of Exogenous H2O2on Germination Rate and Activities of Main Antioxidase in Soybean (Glycine maxL.) Seedlings under Low Temperature
作者:
张立军1; 宋广周1; 白霜1; 魏颖1; 崔震海1; 谢甫绨2
1沈阳农业大学生物科学技术学院，辽宁沈阳110161；
2沈阳农业大学农学院，辽宁沈阳 110161
Author(s):
ZHANG Li-jun1; SONG Guang-zhou1; BAI Shuang1; WEI Ying1; CUI Zhen-hai1; and XIE Fu-ti2
1College of Biological Science and Technology；
2College of Agronomy，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆; 过氧化氢; 萌发; 抗氧化酶
Keywords:
. Soybean(Glycine max L.); H2O2; Germination; Antioxidase
DOI:
10.11861/j.issn.1000-9841.2008.01.0097
文献标志码:
A
摘要:
大豆喜温，对低温敏感，播种后易遭受冷害造成减产。冷害与低温诱发植物活性氧的形成增加并抑制其清除能力有关。但研究表明当环境胁迫诱导活性氧增加的初期，也诱导清除能力的增强，H2O2参与这个过程的诱导。然而外源H2O2处理对大豆种子低温萌发的效应、对抗氧化酶活性的影响以及萌发过程中大豆种子内源H2O2含量的变化还不清楚。以冷敏感型大豆品种沈农8号、沈农9411和耐冷型大豆品种铁丰31、Kottman为材料，用10，20，50，100 和200 mmol L-1的H2O2进行浸种处理后，在低温(5℃)下萌发。当外源H2O2浓度低于50 mmol L-1时，各浓度处理都提高发芽率，降低电解质外渗率，提高超氧化物酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性；当H2O2浓度高于50 mmol L-1时，抗氧化酶活性随H2O2浓度的增加而降低，耐冷品种的抗氧化酶活性始终高于冷敏感品种；在萌发过程中，内源H2O2含量随H2O2处理浓度的升高而增加。表明外源H2O2可能通过大豆内源H2O2含量的提高诱导抗氧化酶活性的增强，从而减轻低温伤害，提高在低温下的萌发能力。
Abstract:
Cold stress induces plants to produce more reactive oxygen species（ROSs)，such as O-2，HO，1O2，H2O2Antioxidases play an important role in scavenging ROSs.The seeds of 2 cold-resistant and 2 cold-sensitive varieties of soybean，after soaked with water，10，20，50，100 and 200 mmol L-1of H2O2for 10 minutes，were distributed over three layers of sterile filter-paper embedded with the same concentrations of H2O2.Then the seeds were germinated under 5℃.The effects of H2O2 on germination rates，concents of endogenous H2O2，activities of antioxidase and cell membrane permeability were determined and analyzed to discuss the mechanism of H2O2.50 mmol L-1or less of H2O2?could increase the germination rates and inhibit the cell membrane permeability and increase antioxidase activities under low temperature in all 4 varieties.Antioxidase activities in cold-resistant varieties were higher than those in the cold-sensitive varieties under all concentrations of H2O2.Endogenous H2O2contents rised in the whole range of concentration of H2O2.Therefore，it is conclude that soaking seeds of soybean with H2O2 can enhance activities of antioxidases，increase their ability to resist cold stress and alleviate cold injury

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备注/Memo

基金项目：辽宁省科技厅重点项目(2001406002)

作者简介：张立军(1960-)，男，教授，博士，从事植物生理教学及研究工作。E-mail：lijunzhang8@yahoo.com.cn；Tel：024-88487163

更新日期/Last Update: 2014-10-09