[1]傅翠真.大豆脂肪氧化酶缺失体检测方法研究[J].大豆科学,2004,23(02):111-113.[doi:10.11861/j.issn.1000-9841.2004.02.0111]
　Fu Cuizhen.THE IDENTIFI ATION TECHNIQUEOF SOYBEAN LIPOXYGENASE[J].Soybean Science,2004,23(02):111-113.[doi:10.11861/j.issn.1000-9841.2004.02.0111]
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大豆脂肪氧化酶缺失体检测方法研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年02期 页码: 111-113 栏目: 出版日期: 2004-05-25
Title:
THE IDENTIFI ATION TECHNIQUEOF SOYBEAN LIPOXYGENASE
文章编号:
1000 -9841(2004)02 -0111 -03
作者:
傅翠真
中国农业科学院作物品种资源研究所, 北京100081
Author(s):
Fu Cuizhen
Inst itute of Crop Germplasm Resources , CA AS , Beij ing 100081
关键词:
大豆; IEF -PAGE ; 脂肪氧化酶缺失体
Keywords:
Soybean ; Lipoxygenase ; IEF -PAGE
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0111
文献标志码:
A
摘要:
薄层等电聚焦电泳技术(IEF)具有准确、快速、分辨率高的特点。作者对IEF -PAGE 电泳制胶及酶染技术进行改进, 应用于大豆脂肪氧化酶同工酶类型鉴定, 获得很好效果, 大大降低实验成本, 为此, 作者研究制定了一套准确、简便的大豆脂肪氧化酶缺失体检测方法。
Abstract:
The grass -bean flavor of soybean food stuff is related to lipoxygenase(Lox ).In processing , thephy sical or chemical methods are used to remove the undesirable flavo r and odo r of soy bean food stuf f , but thesemethods of ten lead to some insolubility of soybean proteins , and decrease the quality of protein .So it is necessary and urgent to select and develop mutant soybean by genetic study .Lipoxygenase lackingmutants (t riple - null o r double -null )soybean could improve nut ritional quality and functionality of soybeanproducts .There are four know n electrophoresis types of soybean seed lipoxy genase (Lox ) .The Lox isozymes havetheir ow n biochemist ry characteristic .IEF -PAGE helps us to identi ty Lox isozyme type and mutants .Wemodif ied the techniques about making gel and staining the gel fo r improving the resolution and reducing the experimentcost .The GelBond PAG sheet was replaced by no rmal glass plate .Use suitable enzyme staining solution.The tests attained remarkably result s and reduced cost .Thus , a technique fo r ident ifying Lox lackingmutants w as developed , that w as rapid and more accuracy .

参考文献/References:

1 　傅翠真, 丁安林, 刘立军, 等.大豆脂肪氧化酶凝胶电泳分析研究[ J] .中国粮油学报, 1993 , 8(4):36 -40.

2 　傅翠真, 徐文英, 苏震.中国大豆脂肪氧化酶缺失种质多样性鉴定研究[ J].中国农业科学, 1997 , 30(1):44 -51.
3 　Hildebrand D F , Versluys R T , Collins G B.Changes in lipoxygenaseisozyme levels during soybean embrgo development [ J] .Plant Science.1991 , 75 :1-8
4　Wang Wanheng , Tet suo Takano , Daisuke shibat a, et al.Molecularbasi s of a null mut at ion in soybean lipoxygenae 2 :S ubst itut ion of glutamine f or an i ron -ligand hi stidine [ J] .Proc.Nat l.Acad .Sci .USA., 1994 , 91 :5828 -5832 .
5 　Wang Wanheng , Tomohiko Kato , Tet suo Takano et al.Two single-base substi tutions involved in alt ering in a paired -box of AAATACin the promot er region of soybean lipoxygenase L -3 gene im pairthe promot er function in t obacco cells [ J] .Plant Science .1995 ,109 :67 -73 .

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备注/Memo

基金项目:农业部“ 948” 项目:大豆近等基因系的引进研究与利用(编号991001 -10 , 1999 -2002 年)　

作者简介:傅翠真(1939 -), 女, 研究员, 主要研究领域为谷物化学、农产品品质鉴定与评价。

更新日期/Last Update: 2016-12-31