[1]乔亚科　李桂兰　王文颇　高书国　毕艳娟　景燕.野生、栽培大豆愈伤组织耐盐性与植株盐胁迫下丙二醛(MDA)含量的关系[J].大豆科学,2003,22(02):0.
　Qiao Yake Li Guilan Wang Wenpo Gao Shuguo Bi Yanjuan Jing Yan.THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEANANDMDA CONTENTOF PLANT GROWN IN SALTY SOIL[J].Soybean Science,2003,22(02):0.
点击复制

野生、栽培大豆愈伤组织耐盐性与植株盐胁迫下丙二醛(MDA)含量的关系

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年02期 页码: 0 栏目: 出版日期: 2003-05-25
Title:
THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEANANDMDA CONTENTOF PLANT GROWN IN SALTY SOIL
作者:
乔亚科　李桂兰　王文颇　高书国　毕艳娟　景燕
河北职业技术师范学院农学系, 昌黎066600
Author(s):
Qiao Yake 　Li Guilan 　Wang Wenpo 　Gao Shuguo 　Bi Yanjuan 　Jing Yan
Hebei Vocat ion - Technical Teachers College , Changl i , 066600
关键词:
野生大豆; 栽培大豆; 愈伤组织; 耐盐性; MDA 含量
Keywords:
Wild soybean ; Cultivated soybeans ; Callus ; Salt tolerance ; MDA
分类号:
S 565.101
文献标志码:
A
摘要:
以野生、栽培大豆共14 份材料的下胚轴作为外植体诱导愈伤组织, 在含不同浓度NaCl 的培养基上鉴定愈伤组织的耐盐性, NaCl 浓度(w/v)分别为0 %(CK), 0 .1 %, 0 .2 %, 0 .3 %, 0 .4 %,0 .5 %, 0 .6 %, 0 .7 %, 0 .8 %;14 份材料的幼苗在NaCl 为0 .3 %的盐土环境中生长25d 后, 测其幼苗的丙二醛(MDA)含量。试验结果表明:愈伤组织水平上, 栽培大豆品种中黄4 、通农5 、吉林35 耐盐性较强, 不同材料之间愈伤组织的耐盐性有明显差异, 耐盐性强的可在0 .8 %NaCl 浓度下生长,耐盐性弱的只能忍受0 .4 %NaCl 浓度;本试验确定大豆愈伤组织耐盐性鉴定适宜的浓度为0 .6 %。在盐胁迫下, 植株MDA 含量在0 .3 %NaCl 条件下均比在CK 土壤中高, 但随胁迫时间的延长而呈现不同的变化趋势。综合大豆植株MDA 含量的变化和愈伤组织的耐盐性之间, 并未发现相关性。
Abstract:
The 14 w ild soybeans and cultivated soybeans w ere studied in this experiment .Hypocotyls w ere usedas explants .Callus w as induced at MS +2 , 4 -D2 .0mg/L +6 -BA0 .5mg/L + sucrose 3 % + agar 0 .8 %.The salt tolerance of callus w as observed on different medium containing 0(as contrast), 0 .1 %, 0 .2 %, 0 .3 %,0 .4 %, 0 .5 %, 0 .6 %, 0 .7 %, 0 .8 %NaCl .The experimental materials were planted also in soil pond containing0 .0 % 、0 .3 %NaCl.The results showed that salt tolerance of callus from Zhonghuang 4 , Tongnong 5 and Jilin35w as st ronger .Among different materials , the difference of salt tolerance w as remarkable .The higher salt tolerancematerials could grow well o n medium containing 0 .8 %NaCl , the lower salt tolerance materials could onlyg row on medium containing 0 .4 %NaCl.Sui table concent ration for identifing salt tolerance of soybean callus was0.6 %NaCl in this experiment.MDA content of plant s in 0 .3 %NaCl w as higher than that in control.MDAcontent of plant s grow n on 0 .4 %NaCl soil pond at different time appeared dif ferent varies wi th the increase ofsalt st ress day s.The co rrelation between MDA content and callus salt tolerance w as not found in this paper .

参考文献/References:

1 　余叔文, 汤章城主编.植物生理与分子生物学[ M] .北京:科学出版社, 2001 .

2 　王仑山, 王鸣刚, 王亚馥.利用组织和细胞培养筛选作物耐盐突变体的研究[ J] .植物学通报, 1996 , 13(2):7-12 .
3 　於丙军, 陈一舞.大豆耐盐性研究进展[ J] .大豆科学, 2000 , 19(2):154-159 .
4 　邵启全, 蒋兴, 周泽其, 等.野生大豆细胞系建立的初步研究[ J] .大豆科学, 1983 , 2(4):316-320 .
5 　陈云昭.大豆耐盐离体筛选分化再生植株的初步研究[ J] , 大豆科学, 1989 , 5(4):339-342 .
6 　刘艳芝, 庄炳昌, 赵桂兰.大豆子叶节组培耐盐突变体筛选[ J] .吉林农业科学, 1998 ,(4):38-39 .
7 　陈云昭, 王玉国.在盐胁迫下获得的大豆愈伤组织及再生植株的生化反应[ J] .大豆科学, 1992 , 11(1):70-73 .
8 　马淑英, 尹田夫, 袁鹰, 等.盐胁迫对大豆发育子叶愈伤组织的生化影响[ J] .大豆科学, 1997 , 16(3):227-232 .
9 　魏爱丽, 百桦, 陈云昭, 等.盐胁迫下大豆初生叶愈伤组织SOD 活性及其同工酶变化的研究[ J] .大豆科学, 1999 , 18(1):85-88 .
10 於丙军, 罗庆云, 曹爱忠, 等.栽培大豆和野生大豆耐盐性及离子效应的比较[ J] .植物资源与环境学报, 2001 , 10(1):25 -29 .
11 於丙军, 罗庆云, 刘友良, 等.盐胁迫对野生大豆生长和离子分布的影响[ J] .作物学报, 2001 , 27(6):776 -780 .
12 Zhiang Hu , Hongxin Wang .Salt t olerance of wi ld soybean(Glycinesoja )in natural populat ions evaluat ed by a new method[ J] .S oybeanGenetics New slet ter, 1997 , 24 , 79 -80 .
13 王爱国.丙二醛作为脂质过氧化指标的探讨[ J] .植物生理学通讯,1986 ,(2):55-57 .
14 陈少裕.膜脂过氧化与植物细胞的伤害[ J] .植物生理学通讯,1991 , 27(2):84-90 .
15 贾恢先, 赵曼容, 马莹.典型盐地植物细胞脂质过氧化伤害与质膜超微结构变化的研究[ J] .西北植物学报.1994 , 14(6):1-5 .
16 王建明, 张作刚, 郭春绒, 等.枯萎病菌对西瓜不同抗感品种丙二醛含量及某些保护酶活性的影响[ J] .植物病理学报, 2001 , 31(2):152-156 .

相似文献/References:

[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
　GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(02):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
　WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(02):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
　CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(02):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
　XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(02):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[7]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
　WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(02):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[8]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[9]吴 倩,张 磊,黄志平,等.转录组测序及其在野生大豆基因资源发掘中的应用[J].大豆科学,2013,32(06):845.[doi:10.11861/j.issn.1000-9841.2013.06.0845]
　WU Qian,ZHANG Lei,HUANG Zhi-ping,et al.Transcription Sequencing and Its Application on Discovering the Gene Resources of Wild Soybean[J].Soybean Science,2013,32(02):845.[doi:10.11861/j.issn.1000-9841.2013.06.0845]
[10]袁翠平,赵洪锟,王玉民,等.利用SSR标记评价抗胞囊线虫野生大豆种质的遗传多样性[J].大豆科学,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
　YUAN Cui-ping,ZHAO Hong-kun,WANG Yu-min,et al.Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers[J].Soybean Science,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
[11]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
　ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(02):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[12]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
　HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(02):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[13]郑世英,郑建峰,张秀玲,等.NaCl胁迫对野生及栽培大豆渗透调节物质含量的影响[J].大豆科学,2011,30(05):786.[doi:10.11861/j.issn.1000-9841.2011.05.0786]
　ZHENG Shi-ying,ZHENG Jian-feng,ZHANG Xiu-ling,et al.Effect of NaCl Stress on the Content of Osmotic Regulation Substances in Glycine soja and Glycine max[J].Soybean Science,2011,30(02):786.[doi:10.11861/j.issn.1000-9841.2011.05.0786]
[14]马光,郭继平,魏淑珍,等.干旱胁迫下野生大豆和栽培大豆生理特性比较[J].大豆科学,2011,30(06):1057.[doi:10.11861/j.issn.1000-9841.2011.06.1057]
　MA Guang,GUO Ji-ping,WEI Shu-zhen,et al.Comparison on Physiological Characteristics of Glycine soja and Glycine max under Drought Stress[J].Soybean Science,2011,30(02):1057.[doi:10.11861/j.issn.1000-9841.2011.06.1057]
[15]李发院,田 芳,张晓可,等.栽培大豆和野生大豆及其回交后代苗期耐盐性分析[J].大豆科学,2012,31(04):593.[doi:10.3969/j.issn.1000-9841.2012.04.016]
　LI Fa-yuan,TIAN Fang,ZHANG Xiao-ke,et al.Analysis of Seedlings Salt Tolerance of Backcross Hybrids of Glycine maxand Glycine soja[J].Soybean Science,2012,31(02):593.[doi:10.3969/j.issn.1000-9841.2012.04.016]
[16]纪展波,蒲伟凤,李桂兰,等.野生大豆、半野生大豆和栽培大豆对苗期干旱胁迫的生理反应[J].大豆科学,2012,31(04):598.[doi:10.3969/j.issn.1000-9841.2012.04.017]
　JI Zhan-bo,PU Wei-feng,LI Gui-lan,et al.Physiological Reaction of Glycine soja,Glycine gracilisand Glycine max to Drought Stress in Seedling Stage[J].Soybean Science,2012,31(02):598.[doi:10.3969/j.issn.1000-9841.2012.04.017]
[17]高小宽,刘国杰,白丽荣.聚乙二醇（PEG）模拟干旱胁迫对野生大豆与栽培大豆萌发的影响[J].大豆科学,2012,31(06):1027.[doi:10.3969/j.issn.1000-9841.2012.06.037]
　GAO Xiao-kuan,LIU Guo-jie,BAI Li-rong.Effect of Polyethylene Glycol(PEG)Simulated Drought Stress on Seed Germination of Wild and Cultivated Soybeans[J].Soybean Science,2012,31(02):1027.[doi:10.3969/j.issn.1000-9841.2012.06.037]
[18]王岚.野生与栽培大豆某些性状的比较及其在大豆育种中的利用[J].大豆科学,2010,29(04):575.[doi:10.11861/j.issn.1000-9841.2010.04.0575]
　WANG Lan.Comparsion of Several Character between Glycine soja and Glycine max and Its Utilization in Soybean Breeding[J].Soybean Science,2010,29(02):575.[doi:10.11861/j.issn.1000-9841.2010.04.0575]
[19]梁江,陈渊,汤复跃,等.利用18S rRNA基因部分序列研究大豆种质资源的进化关系[J].大豆科学,2010,29(04):586.[doi:10.11861/j.issn.1000-9841.2010.04.0586]
　LIANG Jiang,CHEN Yuan,TANG Fu-yue,et al.Reveal the Evolutionary Relationship of Soybean Germplasm by Comparing 18S rRNA Gene Sequences[J].Soybean Science,2010,29(02):586.[doi:10.11861/j.issn.1000-9841.2010.04.0586]
[20]蒲伟凤,李桂兰,张敏,等.干旱胁迫对野生和栽培大豆根系特征及生理指标的影响[J].大豆科学,2010,29(04):615.[doi:10.11861/j.issn.1000-9841.2010.04.0615]
　PU Wei-feng,LI Gui-lan,ZHANG Min,et al.Effects of Drought Stress on Root Characteristics and Physiological Indexes of Glycine soja and Glycine max [J].Soybean Science,2010,29(02):615.[doi:10.11861/j.issn.1000-9841.2010.04.0615]

备注/Memo

基金项目:河北省科技厅项目(99230313)。　

作者简介:乔亚科(1964 -), 男, 硕士, 副教授, 从事大豆遗传资源研究。

更新日期/Last Update: 2016-12-31