大豆脂肪含量QTL基因定位及分析
- Title:
- QTL Mapping and Analysis of Oil Content in Soybean
- 摘要:
- 脂肪含量是大豆重要的品质性状，受多基因控制，为定位大豆脂肪含量相关位点并挖掘候选基因，利用齐小粒1号与绥农76杂交构建的335个单株组成的F2群体，根据中豆芯一号200K基因芯片SNP标记和SSR分子标记的基因型信息，使用德国Bruker MPA近红外光谱仪测定种子脂肪含量，对F2和F3群体进行基因型鉴定，通过IciMapping 4.2软件的复合区间作图法（Inclusive Composite Interval Mapping，ICIM）定位 QTL，结合 SoyBase、Phytozome v13.1 及RNA-seq数据筛选候选基因。结果显示，利用F2群体在15号染色体上获得大小为454.45 kb的定位区间。在此基础上利用F3 群体为材料，加密标记，将区间缩小至164 kb，包含22个基因，共获得了1个QTL，qOil-15-2。区间大小为164 kb，包含22个基因，表型贡献率为6.74%。对基因在Phytozome网站不同组织和转录组数据的表达量进行分析，得到4个候选基因，分别为Glyma.15g025800、Glyma.15g026300、Glyma.15g026400和Glyma.15g026500。研究结果为大豆脂肪基因图位克隆及分子标记辅助育种提供材料和技术支撑，对定向培育高油大豆品种具有重要意义。
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