[1]李文,孙明明,赵丹,等.不同世代间大豆蛋白质和脂肪含量相关QTL的稳定性分析[J].大豆科学,2015,34(01):42-45.[doi:10.11861/j.issn.1000-9841.2015.01.0042]
　LI Wen,SUN Ming-ming,ZHAO Dan,et al.Stability Analysis of QTL Associated with Soybean Protein and Oil Content across Different Generations[J].Soybean Science,2015,34(01):42-45.[doi:10.11861/j.issn.1000-9841.2015.01.0042]
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不同世代间大豆蛋白质和脂肪含量相关QTL的稳定性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 42-45 栏目: 出版日期: 2015-02-25
Title:
Stability Analysis of QTL Associated with Soybean Protein and Oil Content across Different Generations
作者:
李文1; 孙明明2; 赵丹3; 张继雨1; 曹广禄1; 韩英鹏1; 李文滨1; 滕卫丽1
1.东北农业大学 大豆研究所/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030；
?2.黑龙江省农业科学院 信息中心， 黑龙江 哈尔滨 150086； 3.哈尔滨商业大学 食品工程学院， 黑龙江 哈尔滨 150076
Author(s):
LI Wen1;  SUN Ming-ming2;  ZHAO Dan3;  ZHANG Ji-yu1;  CAO Guang-lu1;  HAN Ying-peng1;  LI Wen-bin1;  TENG Wei-li1
1.Soybean Research Institute, Northeast Agricultural University/Key Laboratory of Soybean Biology in Chinese Ministry of Education/ Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Harbin 150030, China; 2.Information Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 3.School of Food Engineering, Harbin University of Commerce, Harbin 150076, China
关键词:
大豆; 遗传群体; 蛋白质含量; 脂肪含量; QTL; 稳定遗传
Keywords:
Soybean;  Genetic population;  Protein content;  Oil content;  QTL;  Genetic stability
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0042
文献标志码:
A
摘要:
以高油大豆东农46为母本，高蛋白大豆L-100为父本，建立F2、F2∶3、F2∶4、F2∶5代群体。应用SSR标记技术，对不同世代在不同地点条件下遗传群体的蛋白质、脂肪含量进行QTL分析。结果表明：不同世代群体的蛋白质含量、脂肪含量均接近于正态分布，其中群体脂肪含量偏向于东农46，蛋白质含量偏向于L-100。在F2∶4代检测到2个与蛋白质含量相关的QTL，分别位于D2和K连锁群，能够解释的表型变异率为1.92%～2.03%，其中位于Satt226附近的QTL在F2、F2∶3和F2∶5代能够稳定地被检测到。在F2∶4代检测到2个与脂肪含量相关的QTL，分别位于F和B2连锁群，能够解释的表型变异率为2.56%～6.98%，其中位于Satt577附近的QTL在F2∶3、F2∶5代能够稳定地被检测到。因此，本研究获得1个与蛋白质含量相关的稳定QTL和1个与脂肪含量相关的稳定QTL。
Abstract:
?In this study, F2, F2∶3, F2∶4?and F2∶5?generation families were formed from a cross between high oil content cultivar Dongnong 46 and the high protein content line L-100. QTL analysis of protein, oil contents for genetic populations were carried via using SSR markers under different generations in different locations. Distribution of the protein and oil content in different generations nearly fitted normal distribution. The expression of the oil content trait in the populations trended to that of the parent Dongnong 46, and the expression of the protein content trait trended to that of the parent L-100. In F2∶4?generation, 2 QTLs of protein content were detected, located in MLG D2,K, and explained the phenotypic variation from 1.92% to 2.03%; especially the QTL in the vicinity of Satt226 in F2, F2∶3?and F2∶5?generations under different environmental conditions could be stably detected. In F2∶4generation, 2 QTLs of oil content were detected, located in MLG F, B2, and explained the phenotypic variation from 2.56% to 6.98%; especially the QTL in the vicinity of Satt577 in F2∶3?and F2∶5?generations under different environmental conditions could be stably detected. This study obtained a stable QTL associated with protein content and oil content, separately.

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备注/Memo

基金项目：国家自然科学基金面上项目（31471517）；国家“十二五”科技支撑计划（2011BAD35B06，2013BAD20B04）；现代农业产业技术体系（CARS-04-PS04）；黑龙江省教育厅科技项目（10531010，12541202，12541210）；黑龙江省自然科学基金（C201123）。

第一作者简介：李文 (1987-)，男，硕士，主要从事大豆品质性状的分子标记研究。E-mail：liwenlevi@163.com。
通讯作者：滕卫丽（1972-），女，博士，研究员，硕导，主要从事大豆遗传育种与生物技术研究。E-mail:twlneau@163.com。

更新日期/Last Update: 2015-04-12