WANG Yu-bin,LIU Wei,ZHANG Yan-wei,et al.Regulation Mechanism in Soybean Seedling Response to Salt Stress of Ethylene[J].Soybean Science,2022,41(05):580-587.[doi:10.11861/j.issn.1000-9841.2022.05.0580]
乙烯对大豆幼苗盐胁迫响应的调控机制研究
- Title:
- Regulation Mechanism in Soybean Seedling Response to Salt Stress of Ethylene
- 关键词:
- 大豆; 盐胁迫; 1-氨基环丙烷-1-羧酸（ACC）; ROS积累; Na+/K+平衡
- Keywords:
- soybean; salt stress; 1-aminocyclopropane-1-carboxylic acid (ACC); ROS accumulation; Na+/K+ equilibrium
- 文献标志码:
- A
- 摘要:
- 为研究乙烯对大豆盐胁迫响应的调控作用，促进乙烯在大豆高效栽培中的应用，以耐盐大豆品种齐黄34为材料，采用水培的方法，外源添加乙烯合成前体1-氨基环丙烷-1-羧酸（1-aminocyclopropane-1-carboxylic acid，ACC）后进行NaCl胁迫处理，设置4种不同处理，测定盐处理对乙烯合成关键限速酶基因表达的影响，乙烯对盐胁迫下大豆幼苗形态建成、渗透调节系统和氧化还原系统的影响，分析乙烯对大豆抗盐的调控效应。结果显示：NaCl处理显著提高不同时间点乙烯合成关键限速酶基因GmACS1、GmACS2和GmACS6的表达，促进乙烯的释放；外源添加ACC可加速盐胁迫下大豆叶片衰老，降低地上部和地下部干物质和叶片叶绿素含量；提高植株地上部和地下部Na+、K+含量、地上部Na+/K+和MDA含量，降低脯氨酸含量；此外，外源添加ACC显著提高盐胁迫下大豆叶片O2?-释放和NADPH氧化酶GmRbohA和GmRbohB基因的表达，降低POD酶活性。研究结果表明乙烯通过调控ROS稳态以及Na+/K+平衡负调控大豆耐盐性。
- Abstract:
- In order to study the regulatory effects of ethylene on the salt stress response of soybean and promote the application of ethylene in high-efficiency soybean cultivation， the salt-tolerant soybean variety Qihuang 34 was used as experimental materials. We used the method of hydroponics， exogenous added of 1-aminocyclopropane-1-carboxylic acid (ACC) and then set NaCl stress treatment. The effects of salt treatment on the gene expression of key rate-limiting enzymes of ethylene synthesis， ethylene on the seedling morphogenesis， osmotic regulation system and oxidation-reduction system were measured to analyse the regulatory effect of ethylene on soybean salt resistance. The results showed that NaCl treatment significantly increased the expression of GmACS1，GmACS2 and GmACS6， the key rate-limiting enzymes of ethylene synthesis at different time points， promoted the evolution of ethylene. Under salt stress， exogenous ACC treatment accelerated the senescence of soybean leaves， decreased dry matter accumulation of shoots and roots， leaf chlorophyll content. Exogenous addition of ACC significantly increased the content of Na+， K+， Na+/K+ and MDA content， and decreased the content of proline under salt stress. In addition， exogenous addition of ACC significantly increased O2?- release and the expression of NADPH oxidase GmRbohA and GmRbohB genes in soybean leaves， but decreased POD enzyme activity under NaCl stress. The results showed that ethylene negatively regulated soybean salt tolerance by regulating ROS homeostasis and Na+/K+ equilibrium.
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备注/Memo
收稿日期：2022-02-03