ZHANG Peng-yu,ZHANG Xiao-rui,HE Ru,et al.Effects of Spraying Frequency and Stage of Amino Acid Fertilizer on Soybean Physiology and Yield[J].Soybean Science,2022,41(05):569-579.[doi:10.11861/j.issn.1000-9841.2022.05.0569]
氨基酸肥喷施次数和时期对大豆生理和产量的影响
- Title:
- Effects of Spraying Frequency and Stage of Amino Acid Fertilizer on Soybean Physiology and Yield
- Keywords:
- soybean; amino acid fertilizer; growth p
- 文献标志码:
- A
- 摘要:
- 为分析氨基酸肥对大豆生长发育和产量的影响,探讨大豆田氨基酸肥的高效施肥次数和时期,以沈农8号(有限型)和沈农9号(亚有限型)两个大豆品种为材料,设置不同生育时期喷施不同次数氨基酸肥处理,对比分析不同生育时期不同处理大豆叶片光合生理、氮代谢、产量及其相关性状等。结果表明:氨基酸肥处理会显著提高大豆的产量,在V4、R1和R3期喷施3次氨基酸肥处理下沈农8号和沈农9号产量显著高于其它处理,分别比不施氨基酸肥对照增产27%和16%。在V4、R1和R3期喷施3次氨基酸肥后两个品种不同时期的净光合生理指标、氮代谢酶活性和氮素积累量均显著高于其它处理。研究表明施用氨基酸肥可以提高大豆植株的光合能力和氮代谢能力,增加单株粒数,从而获得高产,以V4、R1和R3期喷施3次氨基酸肥处理增产率最高。
- Abstract:
- In order to analyze the effect of amino acid fertilizer on the growth, development and yield of soybean, and to explore the frequency and period of high-efficiency fertilization of amino acid fertilizer in soybean fields, two soybean varieties, Shennong 8 (determinate type) and Shennong 9 (semi-determinate type), were used as materials.We set up fertilizer treatments, sprayed different times of amino acid fertilizers in different growth periods, to analyze the photosynthetic physiology, nitrogen metabolism, plant agronomic traits and yield of soybean leaves of different treatments at different growth periods.The results showed that the amino acid fertilizer treatment could significantly increase the yield of soybean. The yields of Shennong 8 and Shennong 9 were significantly higher than those of the other treatments when the amino acid fertilizer was sprayed three times in the V4, R1 and R3 periods,increased by 27% and 16% respectively compared with the control without amino acid fertilizer. The net photosynthetic physiological index, population light energy interception rate, nitrogen metabolism enzyme activity and nitrogen accumulation of the two varieties were significantly higher than other treatments in V4, R1 and R3 stages after three times spraying of amino acid fertilizer. The results showed that the application of amino acid fertilizer could improve the photosynthetic capacity and nitrogen metabolism of soybean plants, increase the seeds number per plant, and thus obtain high yield, and the highest yield was obtained by spraying three times of amino acid fertilizer at V4, R1 and R3 stages.
参考文献/References:
[1]庞庆阳,宣毓龙,蔡旭,等.基于棉粕的氨基酸肥对小麦生长及产量的影响[J].干旱地区农业研究,2017,35(2): 21-24. (PANG Q Y, XUAN Y L, CAI X, et al. Effect of cottonseed amino acid fertilizer on yield and growth of wheat[J]. Agricultural Research in the Arid Areas, 2017, 35(2): 21-24.)[2]张连秋,杨玉岭,朱哲,等.氨基酸肥料在生产中的应用进展[J].农业灾害研究,2014,4(6): 48-49, 55. (ZHANG L Q, YANG Y L, ZHU Z, et al. Application of amino acid fertilizer in production[J]. Journal of Agricultural Catastrophology, 2014, 4(6): 48-49, 55.)[3]YEO A R, CAPORN S J M, FLOWERS T J. The effect of salinity upon photosynthesis in rice (Oryza sativa L.): Gas exchange by individual leaves in relation to their salt content[J]. Journal of Experimental Botany,1985,36(8): 1240-1248.[4]郑宝香.大豆表观光合作用遗传及其与产量关系的研究[D]. 哈尔滨: 东北农业大学, 2008. (ZHENG B X. Study on inheritance of apparent photosynthesis and its relationship with yield in soybean[D]. Harbin: Northeast Forestry University, 2008.)[5]ASHLEY D A, BOERMA H R. Canopy photosynthesis and its association with seed yield in advanced generations of a soybean cross[J]. Crop Science, 1989, 29(4): 1042-1045.[6]MORRISON M J, VOLDENG H D, COBER E R. Agronomic changes from 58 years of genetic improvement of short-season soybean cultivars in Canada[J]. Agronomy Journal, 2000, 92(4): 780-784.[7]常伟. 丛枝菌根化沙枣苗木耐盐胁迫机制研究[D]. 哈尔滨: 东北林业大学, 2020. (CHANG W. Study on the mechanism of salt tolerance of arbuscular Elaeagnus angustifolia seedlings[D]. Harbin: Northeast Forestry University, 2020.)[8]张树生,杨兴明,黄启为,等. 施用氨基酸肥料对连作条件下黄瓜的生物效应及土壤生物性状的影响[J]. 土壤学报, 2007(4): 689-694. (ZHANG S S, YANG X M, HUANG Q W, et al. Effect of application of amino acid fertilizer on biological properties of cucumber plants and soil microorganisms under continuous mono-cropping[J]. Acta Pedologica Sinica, 2007(4): 689-694.)[9]孙新娥,申明,王中华,等.两种叶面肥对日光温室芸豆叶片光合作用和果实品质的影响[J].南京农业大学学报,2011,34(3):37-43. (SUN X E, SHEN M, WANG Z H, et al. Effects of two leaf fertilizers on photosynthesis and fruit quality of kidney bean in solar greenhouse[J]. Journal of Nanjing Agricultural University, 2011, 34(3): 37-43.)[10]张龙, 王森, 刘佳, 等. 氨基酸叶面肥对枣树花期光合作用的影响[J]. 中南林业科技大学学报, 2017, 37(11): 69-75. (ZHANG L, WANG S, LIU J, et al. Amino acid foliar fertilizer effect on Zizyphus jujube flowering photosynthesis[J]. Journal of Central South University of Foresty & Technology, 2017, 37(11): 69-75.)[11]段春慧, 申明, 张治平. 氨基酸肥料对大豆叶片光合作用与产量的影响[J]. 南京农业大学学报, 2012, 35(4): 15-20. (DUAN C H, SHEN M, ZHANG Z P, et al. Effects of amino-acid fertilizer on leaf photosynthesis and yield of soybean[J]. Journal of Nanjing Agricultural University, 2012, 35(4): 15-20.)[12]刘卫琴, 康琅, 汪良驹. ALA 对草莓光合作用的影响及其与抗氧化酶的关系[J]. 西北植物学报, 2006, 26(1): 57-62. (LIU W Q, KANG L, WANG L J. Effects on strawberry photosynthesis and relations to anti-oxidant enzymes of ALA[J]. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(1): 57-62.)[13]康琅, 程云, 汪良驹. 5-氨基乙酰丙酸对秋冬季大棚西瓜叶片光合作用及抗氧化酶活性的影响[J].西北植物学报, 2006, 26 (11): 2297-2301. (KANG L, CHENG Y, WANG L J. Effects of 5-Aminol evulinic Acid(ALA) on the photosynthesis and anti-oxi dative enzymes activities of the leaves of greenhouse water melon in summer and winter[J]. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26 (11): 2297-2301.)[14]TEIXEIRA W F, FAGAN E B, SOARES L H, et al. Seed and foliar application of amino acids improve variables of nitrogen metabolism and productivity in soybean crop[J]. Frontiers in Plant Science, 2018, 9: 396.[15]李强. 不同氮效率玉米品种对氮肥水平与运筹的响应及氮素吸收利用差异[D]. 成都: 四川农业大学, 2017. (LI Q. Differences of maize cultivars with contrasting nitrogen efficiency response to nitrogen level and management on nitrogen uptake and utilization[D]. Chengdu: Sichuan Agricultural University, 2017.) [16]董守坤. 大豆吸收利用氮素规律及相关酶活性研究[D]. 哈尔滨: 东北农业大学, 2008. (DONG S K. Study on the law of nitrogen absorption and utilization and related enzyme activities in soybean[D]. Harbin: Northeast Forestry University, 2008.)[17]张森, 许自成, 李京京, 等. 烟草碳氮代谢及其调控技术研究进展[J].生物技术进展, 2016, 6(5): 312-318. (ZHANG S, XU Z C, LI J J. Advance on carbon and nitrogen metabolism and regulation of tobacco[J]. Current Biotechnology, 2016, 6(5): 312-318.)[18]林清华, 李常健, 张楚富, 等. 水稻谷氨酰胺合成酶同工酶免疫学性质比较研究[J].植物学报, 2000, 42(5): 471-475. (LIN Q H, LI C J, ZHANG C F, et al. Comparative study of immunological properties on glutamine synthetase isozymes in rice plants[J]. Acta Botanica Sinica, 2000, 42(5): 471-475.) [19]ALESSANDRO M, LAURA E, MARCO M, et al. Post-anthesis accumulation and remobilization of dry matter, nitrogen and phosphorus indurum wheat as affected by soil type[J]. European Journal of Agronomy, 2007,26: 179-186.[20]PANG X L, YANG Y M, YU C L, et al. Crop management for increasing rice yield and nitrogen use efficiency in northeast China[J]. Agronomy Journal, 2015, 107(5): 1682-1690. [21]HOTTA Y, TANAKA T, TAKAOKA H, et al. New physiological effects of 5-aminolevulinic acid in plants: The increase of photosynthesis, chlorophyll content, and plant growth[J]. Bioscience, Biotechnology, and Biochemistry, 1997, 61(12): 2025-2028.[22]SOARES L H, DOURADO-NETO D, FAGAN E B, et al. Soybean seed treatment with micronutrients, hormones and amino acids on physiological characteristics of plants[J]. African Journal of Agricultural Research, 2016, 11(35): 3314-3319.[23]蒋舒蕊, 毛莲珍, 赵凯, 等. 氨基酸与腐植酸肥对无土栽培生菜生长及营养品质的影响[J]. 蔬菜, 2021(7): 28-31. (JIANG S R, MAO L Z, ZHAO K, et al. Effects of amino acids fertilizer and humic acid fertilizer on growth and nutritional quality of lettuce in soilless cultivation[J]. Vegetables, 2021(7): 28-31.)
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备注/Memo
收稿日期:2022-03-02