ZHU Kun,ZHAI Ying,YU Hai-wei,et al.Response of Soybean GmNCED5 Gene Under Abiotic Stress and Its Bioinformatics Analysis[J].Soybean Science,2021,40(04):476-481.[doi:10.11861/j.issn.1000-9841.2021.04.0476]
大豆GmNCED5基因非生物胁迫响应及生物信息学分析
- Title:
- Response of Soybean GmNCED5 Gene Under Abiotic Stress and Its Bioinformatics Analysis
- Keywords:
- Soybean; GmNCED5; Abiotic stress; Expression analysis; Bioinformatics analysis; Cis-element
- 文献标志码:
- A
- 摘要:
- 为探究大豆9-顺式-环氧类胡萝卜素双加氧酶（NCED）基因GmNCED5对非生物胁迫的响应及原理，为植物基因工程及大豆抗逆育种提供理论基础，本研究检测GmNCED5在非生物胁迫下的表达量，并对该基因及其启动子序列进行生物信息学分析。实时荧光定量PCR结果显示，干旱、高盐、高温、低温及外源脱落酸（ABA）处理均可以不同程度地诱导GmNCED5在大豆幼苗中表达。GmNCED5基因开放阅读框（ORF）全长1 686 bp，编码561个氨基酸。亚细胞定位预测结果显示，GmNCED5蛋白主要定位于细胞质中。系统进化分析表明GmNCED5蛋白与豇豆VuNCED蛋白的亲缘关系最近。启动子预测分析结果显示，GmNCED5启动子区域含有5种激素响应相关顺式作用元件和3种胁迫响应相关顺式作用元件。由此推测GmNCED5可能通过启动子区域的顺式作用元件响应非生物胁迫。
- Abstract:
- In order to explore the role and mechanism of soybean 9-cis-epoxycarotenoid dioxygenase (NCED) gene GmNCED5 in abiotic stress and provide a theoretical basis for plant genetic engineering and soybean stress resistance breeding, we analyzed the gene and promoter sequence of GmNCED5 with bioinformatics method, and detected its expression levels under abiotic stresses. The results of real-time quantitative PCR showed that drought, high salt, high temperature, low temperature and exogenous abscisic acid (ABA) treatments could induce the expression of GmNCED5 in soybean seedlings to different degrees. The open reading frame (ORF) of GmNCED5 gene was 1 686 bp, encoding 561 amino acids. The prediction results of subcellular localization showed that GmNCED5 protein was mainly localized in the cytoplasm. Phylogenetic analysis showed that GmNCED5 protein was closely related to VuNCED protein in cowpea. The results of promoter prediction analysis showed that the promoter region of GmNCED5 contained five hormone-responsive cis-elements and three stress-responsive cis-elements. These results suggested that GmNCED5 might respond to the abiotic stress via the cis-elements in the promoter region.
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备注/Memo
收稿日期：2021-02-02