[1]赵毅,于翠梅,杨柳,等.野生大豆和不同栽培大豆品种在镉胁迫下种子萌发及幼苗生长的差异[J].大豆科学,2019,38(02):267-273.[doi:10.11861/j.issn.1000-9841.2019.02.0267]
　ZHAO Yi,YU Cui-mei,Yang Liu,et al.Differences of Cadmium Stress on Seed Germination and Seedling Growth in the Wild Soybean and Cultivated Soybeans[J].Soybean Science,2019,38(02):267-273.[doi:10.11861/j.issn.1000-9841.2019.02.0267]
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野生大豆和不同栽培大豆品种在镉胁迫下种子萌发及幼苗生长的差异

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年02期 页码: 267-273 栏目: 出版日期: 2019-03-20
Title:
Differences of Cadmium Stress on Seed Germination and Seedling Growth in the Wild Soybean and Cultivated Soybeans
作者:
赵毅1; 于翠梅1; 杨柳1; 马莲菊2; 谢甫绨1
（1.沈阳农业大学 农学院，辽宁 沈阳 110866；2.沈阳师范大学 生命科学学院，辽宁 沈阳 110034)
Author(s):
ZHAO Yi1;  YU Cui-mei1;  Yang Liu1;  MA Lian-ju2;  XIE Fu-ti1
（1.College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; 2.College of Life Science，Shenyang Normal University, Shenyang 110034, China）
关键词:
栽培大豆; 野生大豆; 镉胁迫; 萌发; 幼苗
Keywords:
Cultivated soybean;  Wild soybean;  Cadmium stress;  Germination;  Seedling
DOI:
10.11861/j.issn.1000-9841.2019.02.0267
文献标志码:
A
摘要:
为探讨野生大豆和不同栽培大豆品种在镉胁迫下种子萌发的差异，筛选优质的大豆耐镉资源,首先比较5种镉浓度（5,10,25,40 mg?L-1）对野生大豆与栽培大豆的种子发芽率、发芽势、发芽指数、活力指数、根长及芽长等参数，筛选出对镉敏感的性状及适中的镉浓度处理；然后以此为基础比较野生大豆1503、超高产大豆（沈农9号和辽豆14）及普通栽培大豆（辽豆16和沈农20）耐镉性差异。结果表明：野生豆1503和栽培豆辽豆16种子的发芽率、发芽势及发芽指数在较高浓度（25或40 mg?L-1）处理时明显下降，其它浓度处理与对照差异不明显或者高于对照，而活力指数、根长及芽长等指标在较低浓度（5或10 mg?L-1）处理时就表现明显的被抑制效应，尤其对根长抑制更明显。活力指数、根长及芽长对镉胁迫更为敏感，可以作为鉴定耐镉材料及监测镉胁迫对植物的影响的重要指标。在5 mg?L-1镉处理下，1503的活力指数、根长、芽长与对照差异不显著，10 mg?L-1时显著下降，辽豆16在5 mg?L-1时3种参数显著低于对照，因此镉浓度5 mg?L-1的处理适中，可用来比较不同品种的耐镉性。在5 mg?L-1镉处理下，按照3个敏感性状的平均值，筛选出耐镉性较强野生豆1503，镉性较弱的栽培豆沈农20，可用于耐镉育种及耐镉机制研究。
Abstract:
To screen the cadmium-tolerant soybean resources, the differences of cadmium stress on the germination and seedling growth of wild soybean and different cultivated soybean seeds were studied.Firstly 5 cadmium concentrations (5,10,25,40 mg?L-1) on the germination rate, germination energy, germination index,vigorin dex, root length and shoot length of wild soybean and cultivated soybean were compared to screen out cadmium sensitive characters and moderate cadmium concentration ,which would be used to compare the cadmium tolerance of wild soybean 1503, super high yield soybean (Shennong 9 and Liaodou 14) and common cultivated soybean (Liaodou 16 and Shennong 20).The results showed that germination rate, germination energy and germination index of the 1503 and Liaodou16 significantly lower than those of control under the treatment of higher concentration(25 or 40 mg?L-1) cadmium.Other treatments were not significantly different from the control group.While the vigor index, root length and shoot length were restrained evidently at lower concentrations (5 or 10 mg?L-1) cadmium and the inhibiting effect of cadmium stress was larger on the root length than the shoot length. The vigor index, root length and bud length were more sensitive to cadmium stress, which could be used as an important indicator to identify cadmium resistant materials and to monitor the degree of cadmium stress. The cadmium concentration of 5 mg?L-1 is moderate, which could be used to compare the cadmium tolerance of different varieties. According to comprehensive effect values in seedlings, 1503 were relatively strong cadmium tolerance, Shennong 20 was relatively weak cadmium tolerance. These soybean materials could be used to explore the tolerance mechanism of soybean under cadmium stress and as important genetic resources in anti-cadmium breeding of soybeans.

参考文献/References:

［1］Yuan X, Wang J, Shang Y E,et al.Health risk assessment of cadmium via dietary intake by adults in China［J］.Journal of the Science of Food and Agriculture,2014, 94:373-380.

［2］Liu T T,Wu P,Wang L H,et al.Response of soybean seed germination to cadmium and acid rain［J］.Biological Trace Elemnet Research, 2011,144:1186-1196.
［3］Xue Z C, Gao H Y, Zhang L T. Effects of cadmium on growth, photosynthetic rate, and chlorophyll content in leaves of soybean seedling［J］.Biologia Plantarum,2013,57 (3): 587-590.
［4］赵云云,钟彩霞,方小龙,等.华南地区夏播大豆品种镉耐性及籽粒镉积累的差异［J］.大豆科学,2013,32(3):336-340.(Zhao Y Y,Zhong C X，Fang X L ,et al.Genotypic differences in tolerance and accumulation to Cd among summer-so-wing soybean varieties in south China［J］.Soybean Science,2013,32(3):336-340.）
［5］Lam H M,Xu X,Liu X,et al.Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection［J］.Nature Genetics,2010, 42:1053-1059.
［6］张煜,李娜娜,丁汉凤,等.野生大豆种质资源及创新应用研究进展［J］.山东农业科学,2012,44(4):31-35.（Zhang Y,Li N N,Ding H F ,et al.Research progress of wild soybean germplasms and utilization［J］.Shandong Agricultural Sciences，2012,44(4):31-35.）
［7］纪展波,蒲伟凤,李桂兰,等.野生大豆、半野生大豆和栽培大豆对苗期干旱胁迫的生理反应［J］.大豆科学,2012,31(4):598-604.(Ji Z B,Pu W F,Li G L,et al.Physiological reaction of Glycine soja，Glycine gracilis and Glycine max to drought stress in seedling stage［J］. Soybean Science,2012,31(4):598-604.)
［8］钟超,李银萍,孙素丽,等.野生大豆资源对大豆疫病抗病性和耐病性鉴定.植物遗传资源学报,2015,16(4):684-690.(Zhong C,Li Y P,Sun S L,et al. Identification of resistance and tolerance to phytophthora sojae in wild soybean germplasm［J］.Journal of Plant Genetic resources, 2015,16(4):684-690.)
［9］Luo Q, Yu B, Liu Y. Differential sensitivity to chloride and sodium ions in seedlings of Glycine max and G. soja under NaCl stress［J］. Journal of Plant Physiology,2003,16:1003-1012.
［10］李娜娜,孔维国,张煜,等.野生大豆耐盐性研究进展［J］.西北植物学报,2012,32(5):1067-1072.(Li N N,Kong W G,Zhang Y,et al. Progress of salt tolerance study in wild soybean［J］.Acta Bot Boreal-Occident Sinica,2012,32(5):1067-1072.)
［11］Qi X,Li M W,Xie M,et al.Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing ［J］.Nature Communications,2014,5:4340.
［12］Xue Z C,Gao H Y.The difference of photosynthetic responses to the cadmium stress between a wild soybean (Glycine soja Sieb. et Zucc.) and a cultivated soybean［J］.Bulletin of Environmental Contamination and Toxicology,2017,99:405-410.
［13］冯君,赵毅,高婷,等.野生和栽培大豆对镉胁迫的响应差异分析［J］.大豆科学,2018,37(5):756-761.(Feng J, Zhao Y, Gao T,et al.The difference of responses to the cadmium stress between a wild soybean and a cultivated soybean［J］.Soybean Science,2018,37(5):756-761.)
［14］樊金娟,刘宇,曹樱迪,等.玉米对镉胁迫的响应及其耐镉机制研究进展［J］.沈阳农业大学学报,2018,49(5):633-640.(Fan J J, Liu Y , Cao Y D, et al.Responses of maize to cadmium stress and mechanisms of cadmium tolerance［J］. Journal of Shenyang Agricultural University,2018,49(5):633-640.)
［15］吴鹏,周青.大豆种子萌发对镉胁迫响应的观察［J］.大豆科学,2009,28(5):853-855.(Wu P,Zhou Q.Response of soybean seed germination to cadmium stress［J］.Soybean Science,2009,28(5):853-855.)
［16］潘高,张合平,刘鹏,等.锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响［J］.草业学报, 2017,26( 11):157-166.(Pan G,Zhang H P,Liu P,et al.Effect of man ganese stress on seed germination seedling physiological and biochemical characteristics of Xanthium sibiricum［J］.Acta Prataculturae Sinica,2017,26(11):157-166.)
［17］俞萍，高凡，刘杰，等. 镉对植物生长的影响和植物耐镉机制研究进展［J］. 中国农学通报，2017，33(11)：89-95. (Yu P, Gao F, Liu J, et al. Effect of Cd on plant growth and its tolerance mechanism［J］. Chinese Agricultural Science Bulletin, 2017, 33(11): 89-95. )
［18］孟桂元,唐婷,周静,等.不同水稻品种种子萌发及根芽生长的耐镉性差异研究［J］.杂交水稻,2015,30(5)：65-69.(Meng G Y,Tang T,Zhou J, et al. Studies on cadmium tolerance differences of seed germination and growth of roots and shoots in different rice varieties［J］.Hybrid Rice,2015,30(5)：65-69.)
［19］孟桂元,唐婷,周静,等.不同玉米品种种子萌发期耐镉性分析［J］.分子植物育种,2016,11(14):3166-3171.(Meng G Y,Tang T,Zhou J, et al. Analysis on cadmium tolerance of different maize varieties during seed germination stage［J］.Molecular Plant Breeding,2016,11(14):3166-3171.)
［20］季玉鸣,李振国,余叔文,等. 镉引起小麦苗逆境乙烯的产生及其和镉的吸收分布［J］．植物生理学报,1989,15(2):159-166．(Ji Y M,Li Z G,Yu S W, et al.Stress ethylene production induced by cadmium in wheat seediings and its relation to cadmium uptake and distribution［J］. Acta Phytophysiologica Sinica,1989,15(2):159-166.)
［21］张治安,王振民,徐克章.Cd 胁迫对萌发大豆种子中活性氧代谢的影响［J］.农业环境科学,2005,24(4):670-673.(Zhang Z A,Wang Z M,Xu K Z. Effect of Cadmium stress on active oxygen metabolism in germinated soybean seeds［J］.Journal of Agro-Environment Science,2005,24(4):670-673.)
［22］于立旭,尚宏芹,张存家,等.外源硫化氢对镉胁迫下黄瓜胚轴和胚根生理生化特性的影响［J］.园艺学报,2011,38(11):2131-2139.(Yu L X,Shang H Q,Zhang C J, et al.Effects of exogenous H2S on the physiological and biochemical characteristics of the cucumber hypocotyl and radicle under cadmium stress［J］.Acta Horticulturae Sinica,2011,38(11):2131-2139.)
［23］Sfaxi-Boushbih A,Chaoui A,Ferjani E E.Cadmium impairs mineral and carbohydrate mobilization during the germination of bean seeds［J］.Ecotoxicology and Environmental Safety,2010,73(6):1123-1129.
［24］张韫璐.镉胁迫对栽培大豆和野生大豆幼苗碳氮代谢影响的研究［D］.沈阳：沈阳师范大学，2018.(Zhang Y L.Effects of Cd stress on carbon and nitrogen metabolism of cultivated and wild soybean［D］.Shenyang: Shenyang Normal University,2018.)
［25］赵玉昆,张惠君,敖雪,等.磷酸二铵对大豆超高产品种养分吸收与利用的影响［J］.中国农业科学 2014,47(12):2326-2334.(Zhao Y K, Zhang H J, Ao X,et al. Effect of different diammonium phosphate levels on nutrient uptake of super-high yielding soybean［J］.Scientia Agricultura Sinica,2014,47(12):2326-2334.)

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　HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(02):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
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　ZHENG Shi-ying,ZHENG Jian-feng,ZHANG Xiu-ling,et al.Effect of NaCl Stress on the Content of Osmotic Regulation Substances in Glycine soja and Glycine max[J].Soybean Science,2011,30(02):786.[doi:10.11861/j.issn.1000-9841.2011.05.0786]
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　MA Guang,GUO Ji-ping,WEI Shu-zhen,et al.Comparison on Physiological Characteristics of Glycine soja and Glycine max under Drought Stress[J].Soybean Science,2011,30(02):1057.[doi:10.11861/j.issn.1000-9841.2011.06.1057]
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　LI Fa-yuan,TIAN Fang,ZHANG Xiao-ke,et al.Analysis of Seedlings Salt Tolerance of Backcross Hybrids of Glycine maxand Glycine soja[J].Soybean Science,2012,31(02):593.[doi:10.3969/j.issn.1000-9841.2012.04.016]
[16]纪展波,蒲伟凤,李桂兰,等.野生大豆、半野生大豆和栽培大豆对苗期干旱胁迫的生理反应[J].大豆科学,2012,31(04):598.[doi:10.3969/j.issn.1000-9841.2012.04.017]
　JI Zhan-bo,PU Wei-feng,LI Gui-lan,et al.Physiological Reaction of Glycine soja,Glycine gracilisand Glycine max to Drought Stress in Seedling Stage[J].Soybean Science,2012,31(02):598.[doi:10.3969/j.issn.1000-9841.2012.04.017]
[17]高小宽,刘国杰,白丽荣.聚乙二醇（PEG）模拟干旱胁迫对野生大豆与栽培大豆萌发的影响[J].大豆科学,2012,31(06):1027.[doi:10.3969/j.issn.1000-9841.2012.06.037]
　GAO Xiao-kuan,LIU Guo-jie,BAI Li-rong.Effect of Polyethylene Glycol(PEG)Simulated Drought Stress on Seed Germination of Wild and Cultivated Soybeans[J].Soybean Science,2012,31(02):1027.[doi:10.3969/j.issn.1000-9841.2012.06.037]
[18]王岚.野生与栽培大豆某些性状的比较及其在大豆育种中的利用[J].大豆科学,2010,29(04):575.[doi:10.11861/j.issn.1000-9841.2010.04.0575]
　WANG Lan.Comparsion of Several Character between Glycine soja and Glycine max and Its Utilization in Soybean Breeding[J].Soybean Science,2010,29(02):575.[doi:10.11861/j.issn.1000-9841.2010.04.0575]
[19]梁江,陈渊,汤复跃,等.利用18S rRNA基因部分序列研究大豆种质资源的进化关系[J].大豆科学,2010,29(04):586.[doi:10.11861/j.issn.1000-9841.2010.04.0586]
　LIANG Jiang,CHEN Yuan,TANG Fu-yue,et al.Reveal the Evolutionary Relationship of Soybean Germplasm by Comparing 18S rRNA Gene Sequences[J].Soybean Science,2010,29(02):586.[doi:10.11861/j.issn.1000-9841.2010.04.0586]
[20]蒲伟凤,李桂兰,张敏,等.干旱胁迫对野生和栽培大豆根系特征及生理指标的影响[J].大豆科学,2010,29(04):615.[doi:10.11861/j.issn.1000-9841.2010.04.0615]
　PU Wei-feng,LI Gui-lan,ZHANG Min,et al.Effects of Drought Stress on Root Characteristics and Physiological Indexes of Glycine soja and Glycine max [J].Soybean Science,2010,29(02):615.[doi:10.11861/j.issn.1000-9841.2010.04.0615]

备注/Memo

收稿日期：2018-12-14

基金项目：辽宁省科技厅项目（2015020762）。
第一作者简介：赵毅（1997-），男，学士，主要从事大豆耐镉研究。E-mail：1943311393@qq.com。
通讯作者：于翠梅（1974-），女，博士，副教授，主要从事大豆耐镉育种研究。E-mail：yucuimei@163.com。

更新日期/Last Update: 2019-04-01