[1]魏思明,陈庆山,蒋洪蔚,等.利用野生大豆染色体片段代换系定位单株粒重QTL[J].大豆科学,2016,35(05):742-747.[doi:10.11861/j.issn.1000-9841.2016.05.0742]
　WEI Si-ming,CHEN Qing-shan,JIANG Hong-wei,et al.TL Mapping for Seed Weight per Plant by using the Wild Soybean Chromosome Segment Substitution Lines[J].Soybean Science,2016,35(05):742-747.[doi:10.11861/j.issn.1000-9841.2016.05.0742]
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利用野生大豆染色体片段代换系定位单株粒重QTL

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年05期 页码: 742-747 栏目: 出版日期: 2016-09-20
Title:
TL Mapping for Seed Weight per Plant by using the Wild Soybean Chromosome Segment Substitution Lines
作者:
魏思明1; 陈庆山1; 蒋洪蔚1; 尹燕斌1; 王丹华1; 胡国华2; 武小霞1; 潘校成3
（1.东北农业大学 农学院，黑龙江 哈尔滨150030； 2.黑龙江省农垦科研育种中心，黑龙江 哈尔滨150090； 3.65301部队副业基地, 黑龙江 五大连池164100）
Author(s):
WEI Si-ming1;  CHEN Qing-shan1;  JIANG Hong-wei2;  YIN Yan-bin1;  WANG Dan-hua1;  HU Guo-hua2;  WU Xiao-xia1;  PAN Xiao-cheng3
（1.Agronomy College, Northeast Agriculture University, Harbin 150030, China; 2.Land Reclamation Research and Breeding Centre of Heilongjiang, Harbin 150090, China; 3.Sideline Base for 65301 Force of Heilongjiang, Wudalianchi 164100,China）
关键词:
大豆; 单株粒重; 染色体片段代换系; QTL定位
Keywords:
Soybean;  Seed weight per plant;  Chromosome segment substitution lines;  QTL mapping
DOI:
10.11861/j.issn.1000-9841.2016.05.0742
文献标志码:
A
摘要:
以野生大豆ZYD00006为供体亲本，黑龙江省主栽品种绥农14为轮回亲本，连续多年回交并自交，构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致，大大提高了QTL定位的准确度。结合单因素方差分析法和独立样本T检验法对群体进行QTL定位，共获得9个单株粒重的QTL，分布于7个连锁群。两种方法中均被检测到的有3个QTL，分别为QSW-J-1、QSW-J-2和QSW-G-1；QSW-G-1和QSW-G-2与已有研究结果相吻合；其余7个QTL为新发现QTL，可能是本材料特有位点；其中QSW-J-1的导入片段长度是7.0 cM，且加性效应值为-2.7 g，可作为继续研究的首选位点。
Abstract:
To study the QTL mapping of seed weight per plant, which is beneficial to increase the yield of soybean. In this study, the BC3F3population is a chromosome segment substitution lines which constituted by 161 lines, with the wild soybean ZYD00006 as donor parent and the cultivar Suinong 14 as recurrent parent, selfing and backcross continuously for several years. The genetic background of this population relatively consistent, which reducing the interference greatly and improving the accuracy of QTL mapping. The QTL mapping was combined by ANOVA Method and T-test for independent samples. A total of 9 QTLs underlying seed weight per plant were detected which distributed on 7 linkage groups.Three QTLs were detected by both methods, including .QSW-J-1, QSW-J-2, QSW-G-1-2 QTL were in accord with known results. Another 7 ones were the new discovery QTL, which should be specific loci in our materias. The fragment length of QSW-J-1 was 7.0 cM, and the additive effects of it is -2.7 g, which could be used as the first choice loci for further study.

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备注/Memo

基金项目：教育部新世纪优秀人才支持计划(NECT-1207-01)；国家青年科学基金项目（31401465）；黑龙江省自然科学基金重点项目（ZD201213）；国家现代农业产业技术体系（CARS-04-02A）；黑龙江省博士后基金（LBH-Z12035）;中国博士后基金（2012M520030）；黑龙江省高校长江后备支持计划项目（2014CJHB004）。第一作者简介：魏思明（1991-），女，硕士，主要从事作物遗传育种研究。E-mail：391294927@qq.com。通讯作者：武小霞（1971-），女，研究员，博导，主要从事大豆生物技术研究。E-mail：Xxwu2012@163.com；潘校成（1973.），男，博士，教授，主要从事大豆遗传育种与生物技术研究。E-mail: soybean2007@126.com

更新日期/Last Update: 2016-09-24