[1]纪丹丹,肖龙,孙培元,等.大豆核孔蛋白GmNup96基因的克隆及生物信息学分析[J].大豆科学,2016,35(05):736-741.[doi:10.11861/j.issn.1000-9841.2016.05.0736]
　JI Dan-dan,XIAO Long,SUN Pei-yuan,et al.Cloning and Bioinformatics Analysis of GmNup96 in Soybean[J].Soybean Science,2016,35(05):736-741.[doi:10.11861/j.issn.1000-9841.2016.05.0736]
点击复制

大豆核孔蛋白GmNup96基因的克隆及生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年05期 页码: 736-741 栏目: 出版日期: 2016-09-20
Title:
Cloning and Bioinformatics Analysis of GmNup96 in Soybean
作者:
纪丹丹1; 肖龙1; 孙培元2; 刘春燕3; 傅永福4; 陈庆山1
（1.东北农业大学 农学院，黑龙江 哈尔滨 150030； 2.黑龙江省农业科学院齐齐哈尔分院，黑龙江 齐齐哈尔 161041； 3.黑龙江省农垦科研育种中心，黑龙江 哈尔滨150050； 4.中国农业科学院 作物科学研究所，北京 100000）
Author(s):
JI Dan-dan1; XIAO Long1; SUN Pei-yuan2; LIU Chun-yan3; FU Yong-fu4; CHEN Qing-shan1
（1.College Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161041,China; 3.Land Reclamation Research and Breeding Centre of Heilongjiang,Harbin 150050,China; 4.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100000,China）
关键词:
大豆; GmNup96; 克隆; 生物信息学分析
Keywords:
Soybean;  GmNup96;  Gene cloning;  Bioinformatics analysis
DOI:
10.11861/j.issn.1000-9841.2016.05.0736
文献标志码:
A
摘要:
从大豆品种天隆1号的叶片中克隆GmNup96基因的cDNA序列，对其编码的氨基酸序列、蛋白质理化性质、一级结构、二级结构、亚细胞定位等进行了生物信息学分析。结果表明：GmNup96基因编码1 022个氨基酸，为具有一定亲水能力的酸性蛋白，不具有信号肽，相对分子量为116-199 7 kDa；二级结构预测结果显示，GmNup96序列存在α-螺旋（46.87%）、无规则卷曲（26.32%）、延伸链（17.03%）和β-转角（9.78%），并无其它二级结构；系统进化树分析表明，大豆-GmNup96-基因与野生大豆、芸豆、绿豆、红小豆之间的亲缘关系更近。
Abstract:
The cDNA of GmNup96 gene in Tianlong 1 was cloned, and its bioinformatics character was analyzed.The results showed that the gene encoded a 116-199 7 kDa protein with 1 022 amino acids ,which was a hydrophilic protein without signal peptide, and the GmNup96 protein contained a conserved autoproteolytic peptidase domain in the N terminus. Through the secondary structure prediction, GmNup96 protein contained alpha-helix(46.87%), beta turn ( 9.78%), extended strand(17.03%) and random coil (26.32%) Phylogenetic analysis of GmNup96 and its homologs with different plant species were conducted by the software MEGA 5-1.The results showed that GmNup96 from Glycine max were near with-Glycine soja, Phaseus vulgaris, Vigna radiata var. radinta and Vigna angularis in genetic relationship

参考文献/References:

［1］Rout M P, Aitchison J D, Suprapto A , et al. The yeast nuclear pore complex: Composition, architecture, and transport mechanism［J］.Journal of Cell Biology, 2000, 148(4):635-651.

［2］Cronshaw J M, Krutchinsky A N, Wenzhu Z, et al. Proteomic analysis of the mammalian nuclear pore complex［J］. Journal of Cell Biology, 2002, 158(5):915-927.
［3］Fiserova J, Kiseleva E, Goldberg M W. Nuclear envelope and nuclear pore complex structure and organization in tobacco BY.2 cells［J］. Plant Journal, 2009, 59(2):243-255.
［4］Goldberg M W, Allen T D.The nuclear pore complex and lamina: Three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy［J］.Journal of Molecular Biology, 1996, 257(4):848-865.
［5］Roberts K, Northcote D H. Structure of the nuclear pore in higher plants［J］. Nature, 1970, 228(5269):385-386.
［6］Yuelin Z, Xin L. A putative nucleoporin 96 is required for both basal defense and constitutive resistance responses mediated by suppressor of npr1-1,constitutive 1［J］. Plant Cell, 2005, 17(4):1306-1316
［7］Geraint P, Sally W, Alex C, et al. The Arabidopsis supressor of auxin resistance proteins are nucleoporins with an important role in hormone signaling and development［J］. Plant Cell, 2006, 18(7):1590-1603.
［8］Yannick J, Chareerat M, Veley K M, et al. The nuclear pore protein AtTPR is required for RNA homeostasis, flowering time, and auxin signaling［J］. Plant Physiology, 2007, 144(3):1383-1390.
［9］Xianfeng M X, Annkatrin R, Sivaramakrishnan M, et al. Nuclear pore anchor, the Arabidopsis homolog of Tpr/Mlp1/Mlp2/megator, is involved in mRNA export and SUMO homeostasis and affects diverse aspects of plant development［J］Plant Cell, 2007,19(5):1537-1548.
［10］Dong C H, Hu X, Tang W, et al. A putative Arabidopsis nucleoporin, AtNUP160, is critical for RNA export and required for plant tolerance to cold stress［J］. Molecular & Cellular Biology, 2006, 26(24):9533-9543.
［11］王旭.拟南芥核孔蛋白Nup96的鉴定及其在开花时间和生物钟调控中的功能分析［D］.北京：中国农业科学院,2014(Wang X.Characterization of Arabidopsis nucleoporin Nup96 and analysis of its roles in flowering time and circadian clock regulation［D］.Beijing: Chinese Academy of Agricultural Sciences,2014.)
［12］刘文瑄,沈应柏,傅永福. 拟南芥核膜孔蛋白-Nup-160基因的突变体表型分析［J］.中国农业科技导报,2015,17(3):29-34.(Liu W X,Shen Y B,Fu Y F.Phenotype analysis of mutants of nucleoporin 160 gene in Arabidopsis［J］.Journal of Agricultural Science and Technology,2015,17(3):29-34.)
［13］Franz C, Askjaer P, Antonin W, et al. Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates［J］. The EMBO Journal, 2005, 24(20):3519-3531.
［14］Guttinger S, Laurell E U. Orchestrating nuclear envelope disassembly and reassembly during mitosis［J］. Nature Reviews Molecular Cell Biology, 2009, 10(3):178-191.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：国家自然科学基金（31271747,31471516）；国家高技术发展计划“863计划”(2013AA102602)；东北农业大学博士启动基金。第一作者简介：纪丹丹（1993-），女，硕士，主要从事大豆分子遗传育种。E-mail：1198477667@qq.com。通讯作者：陈庆山（1973-），男，教授，博导，主要从事大豆遗传育种。E-mail：qshchen@126.com；傅永福（1964-），男，研究员，博导，主要从事作物科学研究。E-mail：fufu19cn@163.com。

更新日期/Last Update: 2016-09-24