[1]杨蕊,禹国龙,沈岩茹,等.大豆GmDＲＲ 与互作基因GmDIP1 的亚细胞定位及原核表达[J].大豆科学,2016,35(04):557-561.[doi:10.11861/j.issn.1000-9841.2016.04.0557]
　YANG ui,YU Guo-long,SHEN Yan-ru,et al.Subcellular Localization and Prokaryotic Expression of GmDＲＲ and Its InteracionGmDIP1 Gene in Soybean[J].Soybean Science,2016,35(04):557-561.[doi:10.11861/j.issn.1000-9841.2016.04.0557]
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大豆GmDＲＲ 与互作基因GmDIP1 的亚细胞定位及原核表达

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 557-561 栏目: 出版日期: 2016-07-20
Title:
Subcellular Localization and Prokaryotic Expression of GmDＲＲ and Its InteracionGmDIP1 Gene in Soybean
作者:
杨蕊1; 禹国龙1; 沈岩茹1; 刘春燕3; 傅永福2; 辛大伟1; 陈庆山1
1. 东北农业大学农学院，黑龙江哈尔滨150030; 2. 中国农业科学院作物科学研究所，北京海淀100000; 3. 黑龙江省农垦科研育种中心，黑龙江哈尔滨150050
Author(s):
YANG Ｒui1; YU Guo-long1; SHEN Yan-ru1; LIU Chun-yan3; FU Yong-fu2; XIN Da-wei1; CHEN Qing-shan1
1. College Agriculture，Northeast Agricultural University，Harbin 150030，China; 2. Institute of Crop Science，Chinese Academy of Agricultural Sciences，Beijing 100000，China; 3. Land Ｒeclamation Ｒesearch and Breeding Centre of Heilongjiang，Harbin 150050，China
关键词:
大豆;  GmDＲＲ;  GmDIP1;  亚细胞定位;  原核表达
Keywords:
Soybean;  GmDＲＲ;  GmDIP1;  Subcellular localization;  Prokaryotic expression
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0557
文献标志码:
A
摘要:
本实验室通过疫霉菌诱导大豆绥农10 号后，获得差异表达蛋白点，通过序列拼接比对和分析，获得一个新的大豆GmDＲＲ 基因，并通过酵母双杂筛选文库的方式获得其互作基因GmDIP1。为进一步明确两个基因关系，对两个基因分别进行亚细胞定位和原核表达以及重组蛋白的纯化。GmDＲＲ 蛋白为外分泌蛋白，定位于细胞膜与细胞质中，GmDIP1 基因定位于线粒体膜上，二者都成功进行了原核表达，从而获得了有表达功能和生物学活性的蛋白。
Abstract:
A differentially expressed protein was separated from soybean Suinong 10 induced by Phytophthora sojae in the previouswork of our lab. A new gene GmDＲＲ was obtained by sequence alignment，comparison and analysis. Then its interactiongene GmDIP1 was screened from cDNA library by yeast two-hybrid. To make sure the interatction relationship，subcellular localizationand prokaryotic expression of the two genes were designed and the recombinatnt proteins were purified. GmDＲＲ is aexocrine protein，positioning in the cell membrane and cytoplasm. GmDIP1 locates on the mitochondrial membrane. Bothgenes successfully expressed in prokaryotic，thus the proteins with expression function and biological activity were obtained.

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备注/Memo

基金项目: 国家自然科学基金( 31271747，31471516) ; 国家高技术研究发展计划“863 计划”( 2013AA102602) ; 东北农业大学博士启动基金。第一作者简介: 杨蕊( 1990-) ，女，硕士，主要从事大豆抗病分子遗传育种研究。E-mail: 704142172@ qq. com。通讯作者: 陈庆山( 1973-) ，男，博士，教授，主要从事大豆遗传育种研究。E-mail: qshchen@126. com;辛大伟( 1981-) ，男，博士，副研究员，主要从事大豆结瘤机制研究。E-mail: xdawei@163. com。

更新日期/Last Update: 2016-08-22