[1]厉志,王曙明,孟凡梅,等.茎中可溶性色素水平法鉴评大豆资源对菌核病的部分抗性[J].大豆科学,2016,35(03):481-488.[doi:10.11861/j.issn.1000-9841.2016.03.0481]
　LI Zhi,WANG Shu-ming,MENG Fan-mei,et al.Soluble Pigment Level in Stems Determining Partly-Ｒesistance to White Mold inSoybean[J].Soybean Science,2016,35(03):481-488.[doi:10.11861/j.issn.1000-9841.2016.03.0481]
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茎中可溶性色素水平法鉴评大豆资源对菌核病的部分抗性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 481-488 栏目: 出版日期: 2016-05-20
Title:
Soluble Pigment Level in Stems Determining Partly-Ｒesistance to White Mold inSoybean
作者:
厉志1; 王曙明1; 孟凡梅1; 刘佳1; 衣志刚1; 张红艳2; 董志敏1
1. 吉林省农业科学院大豆研究所/国家大豆工程技术研究中心，吉林长春130033;?
2. 辽宁省农业技术推广总站，辽宁沈阳110034
Author(s):
LI Zhi1; WANG Shu-ming1; MENG Fan-mei1; LIU Jia1; YI Zhi-gang1; ZHANG Hong-yan2; DONG Zhi-min1
( 1. Soybean Institute，Jilin Academy of Agricultural Sciences /National Engineering Technology Ｒesearch Center for Soybean，Changchun 130033，China;2. Liaoning Agricultural Technology Extending Station，Shenyang 110034，China)
关键词:
大豆;  种质资源;  菌核病部分抗性;  草酸;  茎中可溶性色素水平
Keywords:
Soybean;  Germplasm;  Partly-resistance to white mold;  Oxalic acid;  Soluble pigment level in stems
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0481
文献标志码:
A
摘要:
为了快速、准确地筛选大豆耐菌核病候选资源，采用茎中可溶性色素水平测定法，评价了150 份部分核心种质和国外种质及100 份近15 年东北育成品种的菌核病耐病性，并通过草酸浸根产生的植株萎蔫反应，验证了鉴定结果的准确性; 同时，分析了花色( 茎色) 、叶形、生育期与耐病性的相关性。聚类分析将资源耐病性分为5 个级别，分别是高耐( 3. 20%) 、耐( 7. 60%) 、中感( 21. 60%) 、感病( 46. 00%) 和高感( 21. 60%) ，并筛选出27 份高耐病和耐病的候选资源，其中种质材料中具有相对较多的耐病性资源。草酸浸根植株萎蔫反应与茎中可溶性色素评价结果相符。耐病强度与农艺性状相关分析表明: 叶形和生育期与耐病性不相关，但花色( 茎色) 与耐病性显著相关。可见，在反映草酸致病机制方面，茎中可溶性色素水平测定法能够进行大豆资源菌核病耐病性鉴定和评价; 在遗传多态性丰富的大豆资源中可能会筛选到更多的耐菌核病的资源，可能多以紫花为主。
Abstract:
In order to rapidly and conveniently screen reliable tolerance soybean germplasm，soluble pigment in stems was detectedto identify the 150 soybean germplasm and 100 soybean varieties which were bred in recently 15 years. And the resultswere tested by wilting after plant root invaded with oxalic acid. In addition，the relativity between the leaf shape，flower color，growth days and tolerance intensity to white mold in soybean were analyzed. The identified soybean germplasm were divided intofive grades by clustering analysis，including high tolerance( 3. 20%) ，tolerance( 7. 60%) ，middle susceptible( 21. 60%) ，susceptible( 46%) and high susceptible( 21. 60%) . Twenty-seven soybean germplasm were high tolerant or tolerant grades.Moreover，The result after oxalic acid invading plant root showed that the wilting intensity was corresponded with the solublepigment level in stems. Ｒelative analysis between tolerance intensity and agronomic trait showed，leaf shape and flower color( stem color) were not relative with tolerance intensity，otherwise，flower color( stem color) was significantly relative to toleranceintensity. Above all，the identification method of soluble pigment level in stems can well reflect oxalic acid，soybeangermplasm with highly polymorphic has more potential to be tolerance to white mold in soybean，especially with purple flower.

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备注/Memo

基金项目: 农业部转基因生物新品种培育重大专项( 2014ZX08004-002B-003) ; 吉林省科技发展计划项目( 20140204019NY) 。

第一作者简介: 厉志( 1981-) ，男，硕士，助理研究员，主要从事大豆分子育种研究。E-mail: lizhi527@126. com。
通讯作者: 董志敏( 1978-) ，女，博士，副研究员，主要从事大豆抗病遗传育种研究。E-mail: dongzhimin2005@126. com。

更新日期/Last Update: 2016-07-24