[1]张海鹏,郭娜,牛景萍,等.大豆品种郑97196 对疫霉根腐病的抗性遗传分析及基因定位[J].大豆科学,2016,35(03):373-379.[doi:10.11861/j.issn.1000-9841.2016.03.0373]
　ZHANG Hai-peng,GUO Na,NIU Jing-ping,et al.Genetic Analysis of Ｒesistance to Phytophthora sojae and Mapping of ＲesistanceGene in Soybean Cultivar Zheng 97196[J].Soybean Science,2016,35(03):373-379.[doi:10.11861/j.issn.1000-9841.2016.03.0373]
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大豆品种郑97196 对疫霉根腐病的抗性遗传分析及基因定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 373-379 栏目: 出版日期: 2016-05-20
Title:
Genetic Analysis of Ｒesistance to Phytophthora sojae and Mapping of ＲesistanceGene in Soybean Cultivar Zheng 97196
作者:
张海鹏; 郭娜; 牛景萍; 黄婧; 彭洋; 王海棠; 赵晋铭; 邢邯
南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室( 综合) /作物遗传与种质创新国家重点实验室，江苏南京210095
Author(s):
ZHANG Hai-peng; GUO Na; NIU Jing-ping; HUANG Jing; PENG Yang; WANG Hai-tang; ZHAO Jin-ming; XING Han
(Soybean Ｒesearch Institute of Nanjing Agricultural University /Key Laboratory of Biology and Genetics and Breeding for Soybean( General) ，Ministry ofAgriculture /State Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing 210095，China
关键词:
大豆;  大豆疫霉根腐病;  遗传分析;  抗病基因;  SSＲ 标记
Keywords:
Glycine max;  Phytophthora root rot of soybean;  Genetic analysis;  Ｒesistance gene;  SSＲ markers
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0373
文献标志码:
A
摘要:
由大豆疫霉引起的大豆疫霉根腐病是一种世界性大豆病害，对产量品质影响极大。利用抗病品种进行防治是目前最经济、有效的方法。大豆对疫霉菌株的抗性分为由单基因控制的完全抗性和由多基因控制的部分抗性两种。以对多个大豆疫霉生理小种具有抗性的大豆品种郑97196 作为抗性亲本与大豆感病品系X242003 杂交构建F2∶3重组自交家系群体，用大豆疫霉菌株HeN35 对亲本及F2∶3群体进行抗性遗传分析并利用SSＲ 技术对郑97196 的抗性基因进行定位。结果表明: 郑97196 对大豆疫霉抗性是由一对显性单基因控制的，抗病对感病表现为显性，再用9 种毒力公式不同的疫霉菌株分别接种郑97196 和14 个国内外公认的含有单个抗病基因的大豆品种( 鉴别寄主) ，观察并记录抗性反应类型，结果显示郑97196 的抗性反应类型与14 个鉴别寄主有所不同，推测其可能含有新的抗病基因，暂命名为ＲpsZheng。通过SSＲ 分子作图分析，该基因位于大豆分子遗传图谱N 连锁群上satt485 和satt584 之间，与这两个标记之间的距离分别为2. 1 和3. 7 cM。
Abstract:
Phytophthora root rot of soybean is a worldwide soybean diseases caused by Phytophthora sojae ( P. sojae) ，has agreat impact on the yield and quality. Ｒesistant cultivars is the most economical and effective method to prevent it. SoybeanPhytophthora resistance contain complete resistance controlled by single gene and partial resistance controlled by multiplegenes，in this study the cultivars Zheng 97196 to multiple physiological strain of P. sojae resistant. A population of F2∶3 recombinantinbred lines( ＲIL) derived from the cross of Zheng 97196 × X242003，then the ＲIL was used to analyze the inheritanceof resistance by inoculated P. sojae HeN35 and map the resistance gene to a linkage group. The results showed that the resistanceof Zheng 97196 to P. sojae was controlled by a single gene ( Ｒps) ，and the resistance was dominant to susceptible，then9 kinds of different virulence formula P. sojae were inoculated Zheng 97196 and 14 widely recognized as disease-resistant soybeanvarieties contain a single gene( differential hosts) ，recorded the type of reaction，the results showed Zheng 97196 reactiontype was different from 14 differential hosts，speculated that it may have new resistance genes，temporarily namedＲpsZheng. Basis of linkage analysis with SSＲ markers the gene was located on molecular linkage group N and flanked bysatt485 and satt584 with genetic distances 2. 1 and 3. 7 cM.

参考文献/References:

［1］ Wrather J A，Anderson T Ｒ，Arsyad D M，et al. Soybean diseaseloss estimates for the top ten soybean-producing countries in 1998［J］. Canadian Journal of Plant Pathology，2001，23 ( 2 ) :115-121.

［2］ Wrather J A，Koenning S Ｒ. Estimates of disease effects on soybeanyields in the United States 2003 to 2005［J］. Journal ofNematology，2006，38( 2) : 173-180.
［3］ Schmitthenner A F. Problem sand progress in control of Phytophthoraroot rot of soybean［J］. Plant Disease，1985，69 ( 4 ) :362-368.
［4］ 陈庆河，翁启勇，王源超，等. 福建省大豆疫病病原菌鉴定及其核糖体DNA – ITS 序列分析［J］. 植物病理学报，2004，34( 2) : 112-116. ( Cheng Q H，Weng Q Y，Wang Y C，et al. Phytophthorapathogen identification and ribosomal DNA-ITS sequencein Fujian［J］. Acta Phytopathologica Sinica，2004，34( 2) : 112-116. )
［5］ 王华，李国英，战勇，等. 新疆大豆疫霉根腐病疫病鉴定初报［J］. 新疆农业科学，2006，43( 2) : 106-108. ( Wang H，Li GY，Zhan Y，et al. Phytophthora root rot disease identification preliminaryreport in Xinjiang［J］. Xinjiang Agricultural Sciences，2006，43( 2) : 106-108. )
［6］ 王子迎，王源超，张正光，等. 中国和美国大豆疫霉群体遗传结构的ISSＲ 分析［J］. 生物多样性，2007，15 ( 3) : 215-223.( Wang Z Y，Wang Y C，Zhang Z G，et al. ISSＲ analysis of Chineseand American soybean Phytophthora population genetic structure［J］. Biodiversity Science，2007，15( 3) : 215-223. )
［7］ Dorrance A E，McClure S A，Silva A. Pathogenic diversity ofPhytophthora sojae in Ohio soybean fields［J］. Plant Disease，2006，19( 87) : 139-146.
［8］ Gordon S G，Kowitwanich K，Pipatpongpinyo W，et al. Molecularmarker analysis of soybean plant introductions with resistance toPhytophthora sojae［J］. Phytopathology，2007，97( 1) : 113-118.
［9］ Kilen T C，Hartwig E E，Keeling B L. Inheritance of a secondmajor gene for resistance to Phytophthora root rot in soybeans［J］.Crop Science，1974，14( 2) : 260-262.
［10］ Mueller E H，Athow K L，Laviolette F A. Inheritance of resistanceto four physiologic races of Phytophthora megasperma var. sojae［J］. Phytopathology，1978，68( 9) : 1318-1322.
［11］ Athow K L，Laviolette F A，Mueller E H，et al. A new majorgene for resistance to Phytophthora megasperma var. sojae in soybean［J］. Phytopathology，1980，70( 10) : 977-980.
［12］ Buzzell Ｒ I，Anderson T Ｒ. Another major gene for resistance toPhytophthora megasperma var. sojae in soybean［J］. Soybean GenetNewsl，1981，18( 1) : 30-33.
［13］ Athow K L，Laviolette F A. Ｒps6，a major gene for resistance toPhytophthora megasperma f. Sp. glycinea in soybean［J］. Phytopathology，1982，72( 12) : 1564-1567.
［14］ Anderson T Ｒ，Buzzell Ｒ I. Inheritance and linkage of the Ｒps7gene for resistance to Phytophthora rot of soybean［J］. Plant Disease，1992，76( 9) : 958-959.
［15］ Gordon S G，St Martin S K，Dorrance A E. Ｒps8 maps to a resistancegene rich region on soybean molecular linkage group F［J］.Crop Sciense，2006，46( 1) : 168-173.
［16］ Sandhu D，Schallock K G，Ｒivera-Velez N，et al. Soybean Phytophthoraresistance gene Ｒps8 maps closely to the Ｒps3 region［J］.3 期张海鹏等: 大豆品种郑97196 对疫霉根腐病的抗性遗传分析及基因定位379Journal of Heredity，2005，96( 5) : 536-541.
［17］ Sugimoto T，Yoshida S，Kaga A，et al. Genetic analysis and identificationof DNA markers linked to a novel Phytophthora sojae resistancegene in the Japanese soybean cultivar［J］. Euphytica，2011，182( 1) : 133-145.
［18］ Zhu Z D，Wang H B，Wang X M，et al. Distribution and virulencediversity of Phytophthora sojae in China［J］. AgriculturalSciences in China，2003，36( 7) : 793-799.
［19］ Zhu Z D，Wang X M，Chang Ｒ Z，et al. Identification of races ofPhytophthora sojae and reaction of soybean germplasm resources inHeilongjiang province［J］. Agricultural Sciences in China，2000，33( 1) : 62-67.
［20］ Chen X L，Zhu Z D，Wang X M. Postulation of Phytophthora resistancegenes in soybean cultivars or lines［J］. Agricultural Sciencesin China，2008，41( 4) : 1227-1234.
［21］ 郭娜，胡冠军，赵晋铭，等. 一对单显性大豆抗疫霉根腐病基因的遗传分析及定位［J］. 南京农业大学学报，2015，38( 4) :532-537. ( Guo N，Hu G J，Zhao J M，et al. A pair of Phytophthoraroot rot of soybean single dominant genetic analysis and genemapping［J］. Journal of Nanjing Agricultural University，2015，38( 4) : 532-537. )
［22］ Keim P，Olson T C，Shoemaker Ｒ C. A rapid protocol for isolatingsoybean DNA［J］. Soybean Genetics Newsletter，1988，15( 1) :150-152.
［23］ 刘仁虎，孟金陵. Map Draw，在Excel 中绘制遗传连锁图的宏［J］. 遗传，2003，25( 3) : 317-321. ( Liu Ｒ H，Meng J L. Map-Draw: A microsoft Excel Macro for drawing genetic linkage mapsbased on given genetic linkage data［J］. Hereditas，2003，25( 3) : 317-321. )
［24］ Lin F，Zhao M，Ping J，et al. Molecular mapping of two genesconferring resistance to Phytophthora sojae in a soybean landracePI567139B［J］. Theoretical and Applied Genetics，2013，126( 8) : 2177-2185.［25］ Song Q J，Marek L F，Shoemaker Ｒ C，et al. A new integratedgenetic linkage map of the soybean［J］. Theoretical and AppliedGenetics，2004，109( 1) : 122-128.［26］ 范爱颖，王晓鸣，方小平，等. 大豆品种豫豆25 抗疫霉根腐病基因的鉴定［J］. 作物学报，2009，35 ( 10 ) : 1844-1850.( Fan A Y，Wang X M，Fang X P，et al. Molecular identificationof Phytophthora resistance gene in soybean cultivar Yudou 25［J］.Acta Agronomica Sinica，2009，35( 10) : 1844-1850. )［27］ Weng C，Yu K，Anderson T Ｒ，et al. Mapping genes conferringresistance to Phytophthora root rot of soybean，Ｒps1a and Ｒps7［J］. Journal of Heredity，2001，92( 5) : 442-446.
［28］ Sugimoto T，Yoshida S，Watanabe K，et al. Identification of SSＲmarkers linked to the Phytophthora resistance gene Ｒps1-d in soybean［J］. Plant Breeding，2008，127( 127) : 154-159.
［29］ Wu X L，Zhang B Q，Zhao J M，et al. Identification，genetic analysisand mapping of resistance to Phytophthora sojae of Pm28 insoybean［J］. Agricultural Sciences in China，2011，10 ( 10 ) :1506-1511.
［30］ 刘云飞，万红建，韦艳萍，等. 番茄NBS-LＲＲ 抗病基因家族全基因组分析［J］. 核农学报，2014，28( 5) : 790-799. ( Liu YF，Wan J H，Wei Y P，et al. Tomato NBS-LＲＲ resistance genefamily whole genome analysis［J］. Acta Agriculturae NucleataeSinica，2014，28( 5) : 790-799. )
［31］ Bertrand C Y，Collard and David J. Marker-assisted selection: Anapproach for precision plant breeding in the twenty-first century［J］. Philosophical Transactions of the Ｒoyal Society B-BiologicalSciences，2008，363( 363) : 557-572.

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备注/Memo

基金项目: 国家公益性行业( 农业) 科研专项经费( 201303018) ; 优质高产高油大豆种质创新与新品种选育( BE2013350) ; 江苏省自然科学基金项目( BK2011637 和BK20130684) ; 国家现代农业产业技术体系( CAＲS-04-PS10) ; 江苏省现代作物生产协同创新中心。

第一作者简介: 张海鹏( 1992-) ，男，硕士，主要从事大豆遗传育种研究。E-mail: zhp517@ hotmail. com。
通讯作者: 邢邯( 1963-) ，男，教授，博士生导师，主要从事大豆遗传育种研究。E-mail: hanx@ njau. edu. cn。

更新日期/Last Update: 2016-06-23