[1]许媛,程伟,伍辉军,等.大豆细菌性斑疹病的病原鉴定及大豆新种质抗性评价[J].大豆科学,2015,34(03):463-469.[doi:10.11861/j.issn.1000-9841.2015.03.0463]
　XU Yuan,CHENG Wei,WU Hui-jun,et al.Identification of Pathogen Causing Bacterial Pustule Spot of Soybean and Resistance Evaluation of New Soybean Germplasm[J].Soybean Science,2015,34(03):463-469.[doi:10.11861/j.issn.1000-9841.2015.03.0463]
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大豆细菌性斑疹病的病原鉴定及大豆新种质抗性评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 463-469 栏目: 出版日期: 2015-06-25
Title:
Identification of Pathogen Causing Bacterial Pustule Spot of Soybean and Resistance Evaluation of New Soybean Germplasm
作者:
许媛1; 程伟2; 伍辉军1; 郑蕾琦1; 赵团结2; 高学文1
1.南京农业大学.植物保护学院/华东作物有害生物综合治理农业部重点实验室，江苏 南京 210095;
?2.国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
XU Yuan1; CHENG Wei2; WU Hui-jun1; ZHENG Lei-qi1; ZHAO Tuan-jie2; GAO Xue-wen1
1.College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Pest Management in Crops in Eastern China, Ministry of Agriculture, Nanjing 210995, China;
?2.National Center for Soybean Improvement/ National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
关键词:
大豆; 细菌性斑疹病; 病原菌鉴定; 抗病种质
Keywords:
Soybean;  Xanthomonas axonopodis pv-Glycines;  Pathogen identification;  Resistant gemplasm
DOI:
10.11861/j.issn.1000-9841.2015.03.0463
文献标志码:
A
摘要:
通过对一种近年在江苏省南京市发生严重的大豆叶部病害的病原物进行分离鉴定，检测和评价了本地区大豆新育成品种系及亲本对该病害两个菌株的抗病性。结果表明：通过病原物分离和回接大豆，确定了 4株细菌是引起该病害的病原物。形态学观察、生理生化测定、以及16S rDNA和gyrB聚类分析表明这4株菌株属于地毯黄单胞菌大豆致病变种（Xanthomonas axonopodis pv-glycines）。233份大豆新种质对强致病性菌株C5和B523的抗病性检测结果表明，其对两个菌株的抗性反应存在差异，对C5和B523具有抗病性（高度抗病+中度抗病）的种质分别只占鉴定材料的40.35%和37.77%，说明本地品种总体抗性水平并不高，病害具备流行的前提条件；只有苏鲜21和徐豆16对两个菌株均表现高抗，是抗病育种的优良亲本。
Abstract:
In this study, the pathogen which caused the serious disease of soybean leaves in Jiangsu province was isolated and identified, and a set of newly developed varieties, breeding lines and parents were used to evaluate the disease resistance to the pathogen. The results showed that four strains, which were isolated from different soybean leaf samples could result in the same symptom, were identified as the pathogen.Then, through morphology, biochemical and physiological identification, and 16S rDNA and gyrB cluster analysis, those 4 strains were further classed as the Xanthomonas axonopodis pv-glycines. Different resistance reactions of soybean germplasm to C5 and B523 pathogen strains could be observed. The ratios of soybean accessions with resistance to C5 and B523 were only 40.35% and 37.77%, respectively. It indicated that the resistance level of these soybean lines was not high, and there was precondition for widespreading of the disease. Among the 233 tested accessions, two soybean varieties, Suxian 21 and Xudou 16, showed high resistance to the two strains, and could be used for developing disease-resistant varieties.

参考文献/References:

［1］张金梅,伍辉军,高学文.丁香假单胞大豆致病变种两个III型效应因子的克隆及功能分析［J］. 微生物学通报, 2011, 38(11):1679-1687(Zhang J M, Wu H J, Gao X W. Cloning and functional analysis of two type III-secreted effectors in Pseudomonas syringae pv.glycinea［J］.Microbiology China, 2011, 38(11):1679-1687)

［2］Hong J K, Sung C H, Kim D K, et al. Differential effect of delayed planting on soybean cultivars varying in susceptibility to bacterial pustule and wildfire in Korea［J］.Crop Protection, 2012,42:244-249
［3］Chatnaparat T, Prathuangwong S, Lonescu M, et al. XagR, a LuxR Homolog, Contributes to the virulence of Xanthomonas axonopodis pv. glycines to soybean［J］ Molecular Plant-Microbe Interactions,2012, 25(8):1104-1117
［4］方中达,承河元,朱佳玲.豆科植物Xanthomonas属细菌的比较研究［J］.植物病理学报,1964,7(1):21-30 (Fang Z D, Cheng H Y, Zhu J L. A comparative study of the species of the genus Xanthomonas from leguminous plants［J］. Acta Phytopathologica Sinica,1964,7(1):21-30)
［5］Kaewnum S, Prathuangwong S, Burr T J. Aggressiveness of Xanthomonas axonopodis pvglycines isolates to soybean and hypersensitivity responses by other plants［J］ Plant Pathology,2005,54:409-415?
［6］王芳.大豆细菌性病害的识别与综合防治［J］. 大豆通报,2007(5): 21,34(Wang F. The identification and integrated prevention of bacterial dieases in soybean［J］ Soybean Bulletin, 2007(5): 21,34)
［7］Wrather J A, Anderson T R, Arsyad D M, et al.Soybean disease loss estimates for the top ten soybeanproducing countries in 1998［J］ Canadian Journal of Plant Pathology 2001,23:115-121
［8］Prathuangwong S, Amnuaykit K. Studies on tolerance and rate reducing bacterial pustule of soybean cultivars/lines［J］.Kasetsart Journal(Natural Science),1987,21:408-420
［9］赵海红. 大豆细菌性斑疹病的发病原因及防治方法［J］. 黑龙江农业科学, 2013(11):159-160 (Zhao H H. The causes and prevention methods of soybean bacterial pustule ［J］.Heilongjiang Agricultural Sciences, 2013(11):159-160)
［10］王玉全, 李祥羽, 徐鹏飞, 等. 黑龙江省大豆细菌性斑点病病原菌的分离鉴定及病害分布研究［J］.黑龙江农业科学,2006(6):34-35 (Wang Y Q, Li X Y, Xu P F, et al. Study on the isolation and identification of Pseudomonas syringae pv.glycinea and its distribution in Heilongjiang province［J］. Heilongjiang Agricultural Sciences,2006(6):34-35)
［11］方中达. 植病研究方法［M］. 北京:中国农业出版社, 1998: 156-211 (Fang Z D. Research methods of plant disease［M］. Beijing: China Agriculture Press, 1998: 156-211)
［12］东秀珠, 蔡妙英..常见细菌系统鉴定手册［M］. 北京: 科学出版社,2001:353-369(Dong X Z, Cai M Y. Manual for system identification of common bacteria［M］. Beijing: Science Press, 2001:353-369)
［13］R E布坎南， N E吉本斯.伯杰细菌鉴定手册［M］.8版 北京: 科学出版社1984:313-323 (Bochanan R E, Gibbons N E.Bergey’s manual of determinative bacteriology［M］ 8th ed. Beijing: Science Press, 1984:313-323)
［14］李亚利, 贾迎春, 陈秀荣, 等. 采用16S rDNA鉴定甜瓜细菌性叶斑病菌［J］.植物保护,2007(3): 65-67 (Li Y L, Jia Y C, Chen X R, et al. Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences［J］.Plant Protection, 2007(3): 65-67)
［15］漆艳香, 蒲金基, 张欣, 等. 杧果树生黄单胞叶斑病病原的鉴定［J］. 果树学报,2011,28(4)：645-650. (Qi Y X, Pu J J, Zhang X, et al. Preliminary identification of the pathogen of mango Arboricola- leaf spot ［J］.Journal of Fruit Science,2011,28(4)：645-650)
［16］Kim D H, Kim K H, van K, et al. Finding mapping of a resistance gene to bacterial leaf pustule in soybean［J］.Theoretical and Applied Genetics, 2010,120:1443-1450
［17］Boerma H R, James E S.Soybeans: Improvement, production, and uses［M］. 3rd ed. American Society of Agronomy, 2004: 793-800
［18］Narvel J M, Jakkula L R, Phillips D V, et al.Molecular mapping of Rxp conditioning reaction to bacterial pustule in soybean［J］. Journal of Heredity, 2001,92:267-270
［19］薛鹏奇, 刘芳, 乔俊卿, 等. 油菜菌核病生防芽孢杆菌的分离鉴定及其脂肽化合物分析［J］. 植物保护学报,2011,38(2):127-132(Xue P Q, Liu F, Qiao J Q, et al. Screening of Bacillus strains with high inhibition on rape Sclerotinia disease and its lipopeptide compounds detection［J］. Acta Phytophylacica Sinica, 2011,38(2):127-132)
［20］刘芳, 薛鹏奇, 乔俊卿, 等.西藏低温适生芽孢杆菌的分离鉴定及其抗菌和促生作用［J］ 中国生物防治,2010,26(4):453-460(Liu F, Xue P Q, Qiao J Q, et al. Isolation and identification of a low-temperature-adapted Bacillus in Tibet and its antagonistic and growth-promoting effect［J］.Chinese Journal of Biological Control, 2010,26(4):453-460)

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项（2014ZX08004-004）; 国家高技术研究发展计划“863计划”（2012AA101106）。

第一作者简介：许媛(1991-),女,硕士,主要从事生防与细菌分子生物学研究。E-mail：xyyuan007@163.com。
?通讯作者：高学文(1965-)，男，教授，博导，主要从事生防与细菌分子生物学研究。E-mail：gaoxw@njau.edu.cn。赵团结(1969-),男,教授,博导,主要从事大豆遗传资源与利用研究。E-mail：tjzhao@njau.edu.cn。

更新日期/Last Update: 2015-07-16