[1]翟莹,张军,陈阳,等.大豆GmPRP转基因拟南芥抗盐性鉴定[J].大豆科学,2015,34(02):345-348.[doi:10.11861/j.issn.1000-9841.2015.02.0345]
　ZHAI Ying,ZHANG Jun,CHEN Yang,et al.Salt Tolerance Identification of Soybean GmPRP Transgenic Arabidopsis[J].Soybean Science,2015,34(02):345-348.[doi:10.11861/j.issn.1000-9841.2015.02.0345]
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大豆GmPRP转基因拟南芥抗盐性鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 345-348 栏目: 出版日期: 2015-04-25
Title:
Salt Tolerance Identification of Soybean GmPRP Transgenic Arabidopsis
作者:
翟莹1; 张军2; 陈阳1; 杨晓杰1; 赵艳1; 侯美如2; 田俊1
1齐齐哈尔大学 生命科学与农林学院 遗传重点实验室，黑龙江 齐齐哈尔 161006；
2黑龙江省兽医科学研究所，黑龙江 齐齐哈尔 161006
Author(s):
ZHAI Ying1;  ZHANG Jun2;  CHEN Yang1;  YANG Xiao-jie1;  ZHAO Yan1;  HOU Mei-ru2;  TIAN Jun1
1Key Laboratory of Genetics，College of Life Science and AgroForestry，Qiqihar Univesity，Qiqihar 161006, China；
2Heilongjiang Institute of Veterinary Science,Qiqihar 161006, China
关键词:
大豆; GmPRP; 抗盐性; 拟南芥; 遗传转化
Keywords:
Soyben;  GmPRP;  Salt tolerance;  Arabidopsis;  Genetic transformation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0345
文献标志码:
A
摘要:
为研究大豆脯氨酸富集蛋白基因GmPRP在植物应对盐胁迫中的作用，利用前期克隆的GmPRP基因，以pPZP为植物表达载体，构建了由组成型启动子CaMV35S驱动GmPRP基因的植物表达载体。通过Floral dip法对拟南芥进行遗传转化，获得7株阳性转基因株系。盐处理后，转基因拟南芥的种子萌发率高于野生型且叶片中丙二醛含量极显著低于野生型，表明GmPRP提高了转基因拟南芥的抗盐性。
Abstract:
The soyben proline-rich protein gene GmPRP was cloned early. In order to research the function of GmPRP in response to salt stress, the pPZP.GmPRP plant expression vector was constructed, in which GmPRP gene was driven by constitutive promoter CaMV35S. Seven positive transgenic .Arabidopsis plant lines were obtained through Floral dip genetic transformation. The seed germination rate of GmPRP transgenic Arabidopsis were higher than that of wild-type Arabidopsis under salt treatment GmPRP transgenic Arabidopsismaintained lower level of malondialdehyde compared to that of wild-type Arabidopsis under salt treatment. These results showed that GmPRP transgenic Arabidopsisincreased tolerance to salt compared to wild-type plants.

参考文献/References:

［1］Minorsky P V.The wall becomes surmountable［J］ Plant Physiology, 2002, 128: 345-353

［2］Cassab G I. Plant cell wall proteins［J］Annual Review of Plant Biology, 1998, 49: 281-309
［3］许文亮, 黄耿青, 王秀兰, 等.两个棉花HyPRP基因的分子鉴定与初步表达分析［J］ 作物学报, 2007, 33(7): 1146-1153 (Xu W L, Huang G Q, Wang X L, et al. Molecular characterization and expression analysis of two genes encoding hybrid proline-rich proteins in cotton［J］Acta Agronomica Sinica, 2007, 33(7):1146-1153)
［4］Jose.Estanyol M, Gomis.Ruth F X, Puigdomenech P. The eight-cysteine motif, a versatile structure in plant proteins［J］Plant Physiology and Biochemistry, 2004, 42: 355-365
［5］Gothandam K M, Nalini E, Karthikeyan S,et al.OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance［J］Plant Molecular Biology, 2010, 72: 125-135
［6］Priyanka B, Sekhar K, Reddy V D,et al.Expression of pigeonpea hybrid-proline-rich protein encoding gene (CcHyPRP) in yeast and Arabidopsis affords multiple abiotic stress tolerance［J］Plant Biotechnology Journal, 2010, 8: 76-87
［7］Zhang Y, Schlappi M. Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast cells and form higher order complexes in plants［J］Planta, 2007, 227: 233-243
［8］Hong J C, Nagao R T, Key J L.Developmentally regulated expression of soybean proline-rich cell wall protein genes［J］Plant Cell, 1989, 1: 937-943
［9］He C Y, Zhang J S, Chen S Y. A soybean gene encoding a proline rich protein is regulated by salicylic acid, an endogenous circadian rhythm and by various stresses［J］Theoretical and Applied Genetics, 2002, 104: 1125-1131
［10］翟莹, 雷婷婷, 闫 帆, 等. 大豆逆境诱导基因GmPRP的克隆与表达［J］ 作物学报, 2011, 37(12): 2152-2157(Zhai Y, Lei T T, Yan F, et al.Cloning and expression of a stress-induced GmPRP gene in soybean (Glycine max) ［J］Acta Agronomica Sinica, 2011, 37(12): 2152-2157)
［11］Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana［J］ Plant Journal, 1998, 16: 735-743
［12］Wen X P, Pang X M, Matsuda N, et al. Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers［J］ Transgenic Research, 2008, 17, 251-263
［13］Goodwin W, Pallas J A, Jenkins G I. Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus［J］ Plant Molecular Biology, 1996, 31(4): 771-781
［14］Han Y, GómezVásquez R, Reilly K, et al. Hydroxyproline-rich glycoproteins expressed during stress responses in cassava［J］Euphytica, 2001, 120(1): 59-70
［15］Otte O, Barz W.Characterization and oxidative in vitro cross-linking of an extensin-like protein and a proline-rich protein purified from chickpea cell walls［J］ Phytochemistry, 2000, 53(1): 1-5
［16］Deutch C E, Vinicov I.Post-transcriptional regulation of a salt-inducible alfalfa gene encoding a putative chimeric proline-rich cell wall protein［J］Plant Molecular Biology, 1995, 27(2): 411-418
［17］Bradley D J, Kjellbom P, Lamb C J. Elicitor and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response［J］ Cell, 1992, 70(1): 21-30
［18］Brisson L F, Tenhaken R, Lamb C. Function of oxidative cross-Linking of cell wall structural proteins in plant disease resistance［J］Plant Cell, 1994, 6(12): 1703-1712
［19］Qin L X, Zhang D J, Huang G Q, et al. Cotton GhHyPRP3 encoding a hybrid proline-rich protein is stress inducible and its overexpression in Arabidopsis enhances germination under cold temperature and high salinity stress conditions［J］Acta Physiologiae Plantarum, 2013, 35: 1531-1542-
［20］Parvanova D, Ivanov S, Konstantinova T, et al. Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress［J］Plant Physiology and Bioehemistry, 2004, 42(l): 57.63.

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备注/Memo

基金项目：黑龙江省教育厅科学技术研究项目(12541889)；齐齐哈尔大学青年教师科研启动支持计划项目(2012k-M20)。

第一作者简介：翟莹(1982-)，女，讲师，博士，主要从事大豆分子育种方面的研究。E-mail:fairy39809079@126.com。

更新日期/Last Update: 2015-06-08