[1]陈玉胜,陈全战.大豆水提物对四氯化碳诱导的肝损伤小鼠肝脏抗氧化活力的影响[J].大豆科学,2015,34(02):341-344.[doi:10.11861/j.issn.1000-9841.2015.02.0341]
　CHEN Yu-sheng,CHEN Quan-zhan.Antioxidant Effect of Soybean Water Extracts (SWEs) on Acute Liver Injury induced by CCl4?in Mice[J].Soybean Science,2015,34(02):341-344.[doi:10.11861/j.issn.1000-9841.2015.02.0341]
点击复制

大豆水提物对四氯化碳诱导的肝损伤小鼠肝脏抗氧化活力的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 341-344 栏目: 出版日期: 2015-04-25
Title:
Antioxidant Effect of Soybean Water Extracts (SWEs) on Acute Liver Injury induced by CCl4?in Mice
作者:
陈玉胜; 陈全战
南京晓庄学院 生物化工与环境工程学院，江苏 南京 211171
Author(s):
CHEN Yu-sheng; CHEN Quan-zhan
School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China
关键词:
大豆水提物; 肝损伤; 抗氧化; 联苯双脂
Keywords:
Soybean water extracts (SWEs);  Liver injury;  Antioxidant;  Bifendate
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0341
文献标志码:
A
摘要:
为研究大豆水提物（SWEs）对四氯化碳(CCl4)所致急性肝损伤小鼠的保护作用以及对肝脏抗氧化活力的影响。将60只试验小鼠随机分为正常对照组、模型组、SWEs低（50 mg?kg-1），中（100 mg?kg-1），高（150 mg?kg-1）剂量组及阳性对照组（联苯双脂，100 mg?kg-1)。每天灌胃给药1次，连续7 d。末次给药1 h后，除正常对照组外的其余5组采用10% CCl4?2.0 mL?kg-1剂量一次性腹腔注射，建立小鼠急性肝损伤模型。6 h后摘眼球取血及肝脏，计算各组小鼠的肝指数，比色法测定血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性，及总胆红素（TBIL）和白蛋白(ALB)水平，检测肝组织中丙二醛(MDA)和还原型谷胱甘肽(GSH)水平及一氧化氮合酶（NOS）活性。结果表明: 模型组小鼠肝重和肝指数、血清ALT和AST活性及TBIL水平、肝组织MDA水平及NOS活性均显著高于对照组；而血清ALB及肝组织GSH水平显著低于对照组。与模型组相比，中、高剂量的SWEs及联苯双脂显著降低CCl4所致急性肝损伤小鼠的肝重和肝指数、血清ALT和AST活性及TBIL水平、肝组织NOS活性和MDA水平，并显著提高血清ALB及肝组织GSH水平。大豆水提物对CCl4所致小鼠急性肝损伤具有保护作用，其机制可能与抗氧化作用有关。
Abstract:
To explore the protective effects of soybean water extracts (SWEs) on acute liver damage induced by carbon tetrachloride (CCl4) in mice Sixty mice were randomly divided into control group, model group and SWEs groups at dose of 50, 100 and 150 mg?kg-1, and positive control group (bifendate, 100 mg?kg-1) All the mice were intragastrically administrated the corresponding agents respectively once a day for 7 days One hour after the administration, 10% CCl4?2.0 mL?kg-1 was given to mice in all groups except the control group, with a single intraperitoneal injection to establish the acute liver injury mouse model. Six hours later, mice were sacrificed, and the liver index, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, total bilirubin (TBIL) and albumin (ALB) level in serum were detected; the malondialdehyde (MDA) and glutathione (GSH) levels, and nitric oxide synthase (NOS) activity in liver tissue were also analyzed Results indicated that CCl4?significantly increased the liver weight, liver index, and levels of ALT, AST and TBIL in serum, MDA and NOS in liver tissue; while levels of ALB in serum and GSH in liver tissue were remarkably reduced in model group compared with normal group Compared with model group, 100 and 150 mg?kg-1?SWEs and bifendate notably decreased liver weight and index, levels of ALT, AST and TBIL in serum, NOS activity and MDA level in liver tissue in acute liver injury mice induced by CCl4?treatment; Meanwhile significantly increased levels of ALB in serum and GSH in liver tissue As a conclusion, SWEs has a significant protective effect on CCl4.induced liver injury mice, which might be related to the antioxidant function.

参考文献/References:

［1］Herkel J, Schuchmann M, Tiegs G, et al. Immune mediated liver injury［J］ Journal of Hepatology, 2005, 42 (6): 920-923

［2］许惠仙，汪霞，金延华，等．大豆异黄酮和皂甙对肝癌前病变大鼠血清标志酶及抗氧化活性的影响 ［J］大豆科学，2012，31 (1):82-84． (Xu H X，Wang X，Jin Y H，et al． Effect of soybean isoflavones and saponins on serum marker enzymes and antioxidative activities of rat with hepatic preneoplasia ［J］．Soybean Science，2012,31(1):82-84)
［3］赵育芳，张永生，徐珊, 等．大豆异黄酮对实验性肝纤维化大鼠肝星状细胞活化的影响 ［J］营养学报，2010，32 (3):295-296． (Zhao Y F，Zhang Y S，Xu S，et al． The inhibitory effect of soybean isoflavones on activation of hepatic stellate cell in experimental hepatic fibrosis rats ［J］． Acta Nutrimenta Sinica，2010，32(3):295-296)
［4］杨丽娜，郭英，陈秋丽，等．大豆复合物对四氯化碳致大鼠肝损伤的防护作用 ［J］ 中国老年学杂志，2009，29 (3): 559-560． (Yang L N，Guo Y，Chen Q L，et al． Effect of soybean compounds on liver injury induced by carbon tetrachloride in rats ［J］． Chinese Journal of Gerontology，2009，29(3):559-560)
［5］李建芳，陈必成，余震，等．染料木素对硫代乙酰胺诱导的肝纤维化大鼠PDGF-BB表达的影响 ［J］ 肝胆胰外科杂志，2009，21(2):118-121，125．(Li J F，Chen B C，Yu Z，et al．Effect of genistein on the expression of PDGF.BB in hepatic fibrosis of rats induced by thioacetamide ［J］．Journal of Hepatopancreatobiliary Surgery，2009，21(2):118-121，125)
［6］杨修仕．大豆皂苷对急性酒精性肝损伤的保护作用研究 ［D］太原: 山西大学，2011． ( Yang X S． Protective effects of soysaponins on acute alcohol-induced liver damage ［D］． Taiyuan: Shanxi University，2011)
［7］Brattin W J, Glende E A J, Recknagel R O.Pathological mechanisms in carbon tetrachloride hepatotoxicity［J］Free Radical Biology and Medicine, 1985, 1(1):27-38
［8］Weber L W, Boll M, Stampfl A.Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model［J］ Critical Reviews in Toxicology, 2003; 33 (2):105-36
［9］Shah H, Hartman S P, Weinhouse S. Formation of carbonyl chioride in carbon tetrachloride metabolism by rat liver in vitor［J］Cancer Research, 1979, 39: 39-42
［10］张红，史天陆，王静，等．五乙酰栀子酰苯胺对D-GalN所致小鼠急性肝损伤的保护作用 ［J］ ． 安徽医科大学学报，2013，48(7):786-789．(Zhang H，Shi T L，Wang J，et al． Protective effect of GP.Ac.aniline on acute liver injury induced by D.galactosamine in mice ［J］． Acta Universitatis Medicinalis Anhui，2013，48(7):786-789)
［11］王会, 郭立, 谢文磊．抗氧化剂抗氧化活性的测定方法 ［J］ ．食品与发酵工业，2006, 32（3）：92-99 (Wang H, Guo L, Xie W L.Methods for determining antioxitive activity of antioxidants ［J］ Food and Fermentation Industries, 2006, 32 (3):92-99)
［12］Naik S R Antioxidants and their role in biological functions: An overview［J］ Indian Drugs, 2003, 40 (9):501-516
［13］Michiels C, Raes M, Toussaint O, et al. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress［J］Free Radical Biology and Medicine, 1994, 17 (3):235-248
［14］Minin E A, Buchwalow I B, Wellner M, et al. L.Arginine.NO.cGMP signaling following acute liver injury in the rat［J］Experimetal and Toxicologic Pathology, 2005, 57(2):161-171
［15］Ben A H, Zaouali M A, Alfany.Fernandez I, et al.How to protect liver graft with nitric oxide［J］World Journal of Gastroenterology, 2011, 17 (24): 2879-2889
［16］Saeed N, Khan M R, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts .Torilis leptophyllaL［J］BMC Complementary and Alternative Medicine, 2012, 16(12): 221-228

相似文献/References:

[1]尹学哲,金延华,金梅花,等.大豆异黄酮对内毒素及D-氨基半乳糖所致小鼠急性肝损伤的保护作用[J].大豆科学,2013,32(04):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
　YIN Xue-zhe,JIN Yan-hua,JIN Mei-hua,et al.Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice[J].Soybean Science,2013,32(02):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
[2]尹学哲,金延华,王玉娇,等.大豆异黄酮和皂甙对D-氨基半乳糖所致肝损伤小鼠肝脏抗氧化活力的影响[J].大豆科学,2014,33(01):139.[doi:10.11861/j.issn.1000-9841.2014.01.0139]
　YIN Xuezhe,JIN Yanhua,WANG Yujiao,et al.Effects of Soy Isoflavones and Saponins on Hepatic Antioxidative Activity of Mice with Acute Liver Injury Induced by D galactoamine[J].Soybean Science,2014,33(02):139.[doi:10.11861/j.issn.1000-9841.2014.01.0139]

备注/Memo

基金项目：南京晓庄学院校级重点学科基金项目（XZZDXK201203）。

第一作者简介：陈玉胜(1968-) ，男，博士，高级实验师，主要从事植物生理生化及药理学研究。E-mail:cys8691@163.com。

更新日期/Last Update: 2015-06-08