[1]荣非,王罡,季静,等.利用“微创刷”法获得抗草甘膦转基因大豆[J].大豆科学,2015,34(02):217-223.[doi:10.11861/j.issn.1000-9841.2015.02.0217]
　RONG Fei,WANG Gang,JI Jing,et al.Research of “Minimal Wound Brush” to Acquire Genetically Modified Glyphosate-resistant Soybean[J].Soybean Science,2015,34(02):217-223.[doi:10.11861/j.issn.1000-9841.2015.02.0217]
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利用“微创刷”法获得抗草甘膦转基因大豆

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 217-223 栏目: 出版日期: 2015-04-25
Title:
Research of “Minimal Wound Brush” to Acquire Genetically Modified Glyphosate-resistant Soybean
作者:
荣非1; 王罡1; 季静1; 王萍2; 董歆2; 高海伶1; 曹越平3; 邱丽娟4
1天津大学 环境科学与工程学院，天津 300072；
?2淮海工学院 海洋学院，江苏 连云港 222005；
?3上海交通大学 农业与生物学院，上海 200436；
?4中国农业科学院 作物科学研究所，北京 100081
Author(s):
RONG Fei1; WANG Gang1; JI Jing1; WANG Ping2; DONG Xin2; GAO Hai-ling1; CAO Yue-ping3; QIU Li-juan4
1School of Environmental Science and Engineering, Tianjin University, Tianjin 300072,China;?
2School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005,China;
3School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200436,China;?
4Chinese Academy of Agricultural Sciences, Beijing 100081,China
关键词:
大豆; 胚尖生长点; “微创刷”; EPSPS基因
Keywords:
Soybeans;  Embryonic tips apical point;  “Minimal wound brush”;  EPSPS gene
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0217
文献标志码:
A
摘要:
以发芽3 d的大豆成熟种子胚尖生长点为作用点，利用“微创刷”法将抗草甘膦基因(EPSPS)转入绥农22中，对转化植株T1代进行草甘膦筛选，对筛选后的抗性植株进行PCR检测，得到抗草甘膦转基因大豆。同时研究了不同浓度草甘膦对野生型绥农22与抗草甘膦转基因绥农22大豆植株的影响。结果表明：绥农22 T0代成株率为97.38%，对T1代具有草甘膦抗性的植株进行PCR检测，初步证明EPSPS基因成功转入大豆中，T1代转化效率为6.20%；对野生型绥农22与“微创刷”法获得的转基因绥农22大豆在不同浓度草甘膦进行相关生理指标测定，抗草甘膦转基因绥农22大豆在不同浓度草甘膦作用下叶片叶绿素含量指数、光合速率高于野生型绥农22大豆，莽草酸含量低于野生型绥农22大豆，进一步证明了大豆抗性植株对草甘膦的抗性。
Abstract:
Growing points of embryonic tips from mature soybeans after 3 days post germination were used as a receiving point of Agrobacteria carrying recombinated vectors for the transformation of glyphosate-resistant gene (EPSPS) into soybeans (Suinong 22) by using “minimal wound brush” method, and transformed T1?plants were screened by glyphosate and tested by PCR. Chlorophyll content index, shikimic acid concentration, and photosynthetic rate of wild-type and transgenic soybean plants were determined.The results showed that surviving rate of T0 soybean plants was 97.38%, transformation rate for T1?soybean plants was 6.20%.Meansurements of related physiological factors from wild-type and transgenic plants were taken and it was found that chlorophyll content index and photosynthetic rate were higher in trangenic plants than wild types; meanwhile, shikimic acid concentration were much lower in transgenic plants than wild types.The above physicological results confirmed the resistance of glyphosate in transgenic soybeans plants.

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项（2011ZX08004-001-04）。

第一作者简介：荣非（1990-），男，硕士，主要从事植物转基因研究。E-mail:rongfei19900@gmail.com。
通讯作者：王罡（1964-），男，博导，教授，主要从事植物耐盐碱基因、植物基因工程研究。E-mail:wanggangtjdx@126.com；季静（1965-），女，博导，教授，主要从事遗传学、基因工程和细胞工程研究。

更新日期/Last Update: 2015-06-07