[1]舒英杰,陶源,王爽,等.大豆HB基因GmSbh1的分离与生物信息学分析[J].大豆科学,2015,34(02):212-216,223.[doi:10.11861/j.issn.1000-9841.2015.02.0212]
　SHU Ying-jie,TAO Yuan,WANG Shuang,et al.Isolation and Bioinformatics Analysis of GmSbh1 in Soybean[J].Soybean Science,2015,34(02):212-216,223.[doi:10.11861/j.issn.1000-9841.2015.02.0212]
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大豆HB基因GmSbh1的分离与生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 212-216,223 栏目: 出版日期: 2015-04-25
Title:
Isolation and Bioinformatics Analysis of GmSbh1 in Soybean
作者:
舒英杰1; 2; 陶源1; 王爽1; 黄丽燕1; 陈明1; 麻浩1
1南京农业大学 作物遗传与种质创新国家重点实验室，江苏 南京 210095；
2安徽科技学院 农学院，安徽 凤阳 233100.
Author(s):
SHU Ying-jie1; 2;  TAO Yuan1;  WANG Shuang1;  HUANG Li-yan1;  CHEN Ming1;  MA Hao1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China；?
2College of Agriculture, Anhui Science & Technology University, Fengyang 233100, China
关键词:
大豆; 同源异型盒基因; 基因分离; 生物信息学分析
Keywords:
Soybean; Homeobox gene; Gene isolation; Bioinformatics analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0212
文献标志码:
A
摘要:
从大豆叶片中分离到GmSbh1 基因cDNA的完整开放阅读框（ORF）序列，对其编码的氨基酸序列、蛋白质一级、二级结构及互作蛋白等进行了生物信息学分析。结果表明：在GmSbh1 基因cDNA序列的第156~1 293 bp处有一个完整的ORF，编码379个氨基酸；GmSbh1具有同源异型盒（homeoboxbox，HB）基因家族典型的KNOX1、KNOX2、ELK和Homeodomain (HD) 结构域；检索到18条与GmSbh1核酸序列一致性大于80%的序列；GmSBH1为亲水性蛋白，不具有信号肽，分子量为42.37 kDa，其中丝氨酸（Ser）、天冬氨酸（Asn）和亮氨酸（Leu）分别为占10%、9.8%和8.4%，可能发生磷酸化的位点分别在Ser（13个）、酪氨酸（Tyr）（2个）和苏氨酸（Thr）（1个）上；二级结构预测结果显示，GmSBH1序列存在α-螺旋（占39.31%）、β-转角（占5.28 %）、延伸链（占8.44%）和无规则卷曲（占46.97 %），不存在跨膜螺旋；蛋白互作预测表明，GmSBH1与拟南芥中的RPL、BEL1、AT4G32980.1-P、BLH2和BLH3等蛋白的互作程度较高，其中与BEL1共表达的几率较高。
Abstract:
The cDNA sequences of GmSbh1-gene in soybean were isolated, and its bioinformatics character was analyzed. The results showed that there was an intact ORF in 156-1 293 bp , and it encoded a 42.37 kDa protein with 379 amino acids.The GmSBH1 protein contains the KNOX1, KNOX2, ELK and Homeodomain (HD).There were 18 homologous sequences that have more than 80% identity with GmSbh1 GmSBH1 was a hydrophilic protein without signal peptide and contained 10% Ser, 9.8% Asn and 8.4% Leu, the phosphorylation sites were located at Ser(13), Tyr(2) and Thr(1), respectively.Secondary structure prediction results showed that the GmSBH1 protein contains alpha-helix(39.31%), beta turn (5.28%), extended strand(8.44%) and random coil (46.97%), but without transmembrane helical structure. The probability of GmSH1 interacted with RPL, BEL1, AT4G32980.1-P, BLH2 and BLH3 was higher, and coexpression with BEL1 in .Arabibidopsis.

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备注/Memo

基金项目：国家自然科学基金（31101212，31171572）；安徽省教育厅高等学校省级优秀青年人才基金（2012SQRL143）。

第一作者简介：舒英杰（1977-），男，博士，副教授，主要从事大豆种子发育与遗传控制研究。E-mail：shuyj@ahstu.edu.cn。
通讯作者：麻浩（1965-），男，教授，博导，主要从事种子科学与大豆遗传育种研究。E-mail：lq.ncsi@njau.edu.cn。

更新日期/Last Update: 2015-06-07