College of Life Science/Key Lab of Plant Biotechnology in Universities of Shandong,Qingdao Agricultural University,Qingdao 09,et al.WU Yan-yan, DAI De-yan, CAI Chun-mei[J].Soybean Science,2015,34(01):112-115.[doi:10.11861/j.issn.1000-9841.2015.01.0112]
一种改良的大豆DNA提取方法
- Title:
- WU Yan-yan, DAI De-yan, CAI Chun-mei
- Keywords:
- It is difficult to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research; because of a great deal of protein; polysaccharides; hydroxybenzenes and esters. A modified UREA (mUREA) protocol for extracting soybean DNA was provided in this paper. We precipitated DNA with isopropanol and washed DNA pellet with washing buffer. Using soybean leaves and seeds as material; compared mUREA method with CTAB and SDS methods. The results showed that the quality and yield of DNA from soybean leaves were highly significant than using mUREA method. And using soybean seeds as material; the purity of DNA from mUREA was better than the other two methods. So mUREA method yielded much more DNA of high quality for soybean leaves and seeds that was suitable for various molecular operations.
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆组织中含有较多的蛋白质、糖类、酚类和脂类物质，要从中提取高质量的DNA比较困难。针对这一情况提出一种改良UREA大豆DNA提取方法（mUREA），该方法用异丙醇沉淀DNA，并配制washing buffer洗涤DNA沉淀。并以大豆叶片和种子为材料，将mUREA法与CTAB法和SDS法进行对比。结果表明，以大豆叶片为材料时，mUREA法提取的DNA产率最高，质量最好；以大豆种子为材料时，mUREA法提取的DNA产率略低于SDS法，但纯度最高。综合来看，mUREA法是一种高效的大豆基因组DNA提取法，从大豆种子和叶片中提取的DNA浓度和纯度都较高，能满足后续各种分子生物学的要求。
- Abstract:
- A Modified UREA Protocol for Soybean DNA Extraction
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备注/Memo
基金项目：国家自然科学基金（31071444，31371647）；山东省优秀中青年科学家科研奖励基金（BS2011NY006）；青岛农业大学高层次人才启动基金（630908, 631304）。