CUI Zhan-li,YANG Hui-qing,LI Ping,et al.Related Indexes of Dual Symbiotic of Different Soybean Varieties with Indigenous Arbuscular Mycorrhizal Fungi and Rhizobia in Seedling[J].Soybean Science,2014,33(01):83-86.[doi:10.11861/j.issn.1000-9841.2014.01.0083]
不同大豆品种苗期与土著根瘤菌、丛枝菌根真菌双共生相关指标的分析
- Title:
- Related Indexes of Dual Symbiotic of Different Soybean Varieties with Indigenous Arbuscular Mycorrhizal Fungi and Rhizobia in Seedling
- 文章编号:
- 1000-9841(2014)01-0083-04
- Keywords:
- Soybean; Indigenous arbuscular mycorrhizal fungi; Indigenous rhizobia; Dual symbiosis; Biomass
- 分类号:
- S565.1,S154.1
- 文献标志码:
- A
- 摘要:
- 为了筛选与土著根瘤菌、丛枝菌根真菌(AMF)高效双共生的大豆品种,在盆栽条件下,研究了不同类型的7个大豆品种在苗期与土著根瘤菌、AMF形成双共生体系及大豆生物量的状况。结果表明:7个大豆品种的根系AMF侵染率、根际土壤AMF孢子数、根瘤数量有显著(P<0.05)或极显著(P<0.01)差异;垦鉴豆43在V2和V4期与土著根瘤菌和AMF能较好地形成双共生体,根段AMF侵染率和单株根瘤数均较高,根瘤与丛枝菌根(AM)形成相互制约较小,而黑农35的AM对根瘤形成有较大的制约作用,根段AMF侵染率较高,单株根瘤数最低;AM和根瘤的形成对大豆生物量积累的响应或影响在7个品种中表现不一致,垦鉴豆43和抗线2号的AMF侵染率、根瘤数量和生物量积累相对较高。
- Abstract:
- In order to screen soybean varieties possessed of efficient dual symbiosis system formed by indigenous rhizobia,indigenous arbuscular mycorrhizal fungi(AMF)for soybean production,the dual symbiotic systemes of seven soybean varieties with both indigenous rhizobia and arbuscular AM fungi and status of soybean biomass at the seedling stage were studied under potting conditions in the paper.The results were that there were significant differences (P<0.05 or P<0.01)in mycorrhizal infection rate,spore number and root nodule number per plant of seven soybean varieties in the seedling.In V2 and V4 period,Kenjiandou 43 could form a better dual symbiosis with indigenous rhizobia and arbuscular mycorrhizal fungi,and had a little restraining effect between nodule and AM formation.But AM of Heinong 35 had a greater inhibition on root nodules formation.The reaction or impact of starting up of mycorrhizal colonization and root nodule on the biomass of seven soybean varieties were inconsistent.Kenjiandou 43 and Kangxian 2 had more mycorrhizal infection rate of soybean roots,spore number and root nodule number per plant.
参考文献/References:
[1]Azcón-Aguilar C,Azcón R,Barea J M.Endomycorrhizal fungi and Rhizobium?as biological fertilizers for Medicago sativa?in normal cultivation[J].Nature,1979,279:325-327. [2]Antunes P M,de Varennes A,Rajcan I,et al.Accumulation of specific flavonoids in soybean(Glycine max?L.Merr.)as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum?(Kirchner)Jordan[J].Soil Biology and Biochemistry,2006,38:1234-1242. [3]申鸿,刘于,李晓林,等.丛枝菌根真菌(Glomus caledonium?)对铜污染土壤生物修复机理初探[J].植物营养与肥料学报,2005,11(2):199-204.(Shen H,Liu Y,Li X L,et al. Influence of arbuscular mycorrhizal fungus(?Glomus caledonium?)on maize seedlings grown in copper contaminated soil[J].Plant Nutrition and Fertilizer Science,2005,11(2):199-204.) [4]赵丹丹,李涛,赵之伟.丛枝菌根真菌-豆科植物-根瘤菌共生体系的研究进展[J].生态学杂志,2006,25(3):327-333.(Zhao D D,Li T,Zhao Z W.Research advances in arbuscular mycorrhizal fungi legumes-rhizobia symbiosis[J].Chinese Journal of Ecology,2006,25(3):327-333.) [5]李淑敏,李隆,张福锁.丛枝菌根真菌和根瘤菌对蚕豆吸收磷和氮的促进作用[J].中国农业大学学报,2004,9(1):11-15.(Li S M,Li L,Zhang F S.Enhancing phosphorus and nitrogen uptake of faba bean by inoculating arbuscular mycorrhizal fungus and Rhizobium leguminosarum?[J].?Journal of China Agricultural University,2004,9(1):11-15.) [6]Han T X,Tian C F,Wang E T,et al.Associations among rhizobial genomic species,nod genes,and host plants based on the analysis of symbiosis of indigenous rhizobia and wild legumes native to Xinjiang[J].Microbial Ecology,2010,59:311-323. [7]Han T X,Wang E T,Han L L,et al.Molecular diversity and phylogeny of rhizobia associated with wild legumes native to Xinjiang,China[J].Systematic and Applied Microbiology,2008,31:287-301. [8]Mosse B.Advances in the study of vesicular-arbuscular mycorrhiza[J].Annual Review of Phytopathology,1973,11:171-196. [9]Galleguillos C,Aguirre C,Barea J M,et al.Growth promoting effect of two ?Sinorhizobium meliloti??strains(a wildtype and its genetically modified derivative)on a non-legume plant species in specific interaction with two arbuscular mycorrhiza fungi[J].Plant Science,2000,159:57-63. [10]Shimoda Y,Han L,Yamazaki T,et al.Rhizobial and fungal symbioses show different requirements for calmodulin binding to calcium calmodulin-dependent protein kinase in?Lotus japonicus?[J].Plant Cell,2012,24:304-321. [11]董昌金,赵斌.丛枝菌根真菌与根瘤菌互作及类黄酮对互作效果的影响[J].应用生态学报,2004,15(9):1585-1588.(Dong C J,Zhao B.Interaction between AM fungi and ?Rhizobium?and effects of fiavonoids on it[J].Chinese Journal of Applied Ecology,2004,15(9):1585-1588.) [12]van Brussel A A,Tak T,Boot K J,et al.Autoregulation of root nodule formation:signals of both symbiotic partners studied in a split root system of?Vicia sativa?subsp.nigra[J].Molecular Plant-Microbe Interactions,2002,15:341-349. [13]陈文新,汪恩涛.中国根瘤菌[M].北京:科学出版社,2011:400-402.(Chen W Y,Wang E T.China rhizobium[M].Beijing:Science Press,2011:400-402.) [14]Daniels B A,Skipper H D.Methods for the recovery and quantitative estimation of propagules from soil[M].St.Paul:The American Phytopathology Society Press,1982:29-35. [15]Tisserant B,Gianinazzi S,Gianinazzi-pearson V.Relationships between lateral root order,arbuscular mycorrhiza development,and the physiological state of the symbiotic fungus in Platanus acerifolia[J].Canadian Journal of Botany,1996,74:1947-1955. [16]唐明.菌根真菌提高植物耐盐性[M].北京:科学出版社,2010:49.(Tang M.Salt tolerance of plant induced by arbuscular mycorrhizal fungi[M].Beijing:Science Press,2010:49.) [17]Sun S B,Wang J J,Zhu L L,et al.An active factor from tomato root exudates plays an important role in efficient establishment of mycorrhizal symbiosis[J].Plos One,2012,7:1-7. [18]Tajini F,Suriyakup P,Vailhe H,et al.Assess suitability of hydroa-eroponic culture to establish tripartite symbiosis between different AM fungi species,beans,and rhizobia[J].BMC Plant Biology,2009,9:1-11. [19]Catford J G,Staehelin C,Lerat S,et al.Suppression of arbuscular mycorrhizal colonization and nodulation in split-root systems of alfalfa after pre-inoculation and treatment with nod factors[J].Journal of Experimental Botany,2003,54:1481-1487.?
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
基金项目:黑龙江省教育厅面上项目(11541253) 。